中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2661-2664.doi: 10.3969/j.issn.1673-8225.2012.15.001

• 骨组织构建 bone tissue construction •    下一篇

同种异体血清体外培养兔膝关节软骨细胞的增殖*★◆

曾令员，王世东，贺冬冬，王宇泽，段王平，卫小春   

  1. 山西医科大学第二医院骨科，骨与软组织损伤修复山西省重点实验室，山西省太原市030001
  • 收稿日期:2012-02-02 修回日期:2012-02-11 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 卫小春，教授，博士生导师，山西医科大学第二医院骨科，骨与软组织损伤修复山西省重点实验室，山西省太原市 030001 weixiaochun06@yahoo.com.cn
  • 作者简介:曾令员★，男，1985年生，安徽省怀宁县人，汉族，山西医科大学在读硕士，医师，主要从事关节软骨损伤研究。zlyahaq@163.com
  • 基金资助:

    国家自然科学基金资助项目(81071495)，诱导性Ihh基因敲除对软骨退变治疗作用的研究。

Effects of allogeneic serum on proliferation of rabbit articular chondrocytes cultured in vitro  

Zeng Ling-yuan, Wang Shi-dong, He Dong-dong, Wang Yu-ze, Duan Wang-ping, Wei Xiao-chun   

  1. Department of Orthopedics, the Second Hospital of Shanxi Medical University, Shanxi Key Laboratory of Bone & Soft Tissue Injury Repair, Taiyuan  030001, Shanxi Province, China
  • Received:2012-02-02 Revised:2012-02-11 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Wei Xiao-chun, Professor, Doctoral supervisor, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Shanxi Key Laboratory of Bone & Soft Tissue Injury Repair, Taiyuan 030001, Shanxi Province, China weixiaochun06@yahoo.com.cn
  • About author:Zeng Ling-yuan★, Studying for master’s degree, Physician, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Shanxi Key Laboratory of Bone & Soft Tissue Injury Repair, Taiyuan 030001, Shanxi Province, China zlyahaq@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071495*

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摘要：

背景：兔关节软骨细胞体外单层培养采用胎牛血清培养基易去分化，有必要寻找合适培养基来提高兔关节软骨细胞培养质量。
目的：观察同种异体兔血清对体外培养的兔膝关节软骨细胞增殖能力的影响。
方法：以0.4%链霉蛋白酶和0.025%Ⅱ型胶原酶分离成年兔膝关节软骨细胞，将获得的软骨细胞随机分为实验组和对照组。实验组以体积分数10%异体兔血清+DMEM/F12培养；对照组以体积分数10%胎牛血清+DMEM/F12培养，传代培养至4代。
结果与结论：实验组前4代软骨细胞增殖较对照组慢,但软骨细胞形态未发生明显改变而对照组软骨细胞出现去分化现象。提示体积分数10%异体兔血清培养利于维持软骨细胞增殖和形态的稳定，是较好的获取大量优良软骨细胞的体外培养方式。
 

关键词: 同种异体血清, 软骨细胞, 增殖, 细胞培养, 兔

Abstract:

BACKGROUND: Because chondrocytes are easy to dedifferentiate when they are cultured in vitro with fetal bovine serum, it is necessary to find a suitable medium to improve the quality of rabbit articular chondrocytes.
OBJECTIVE: To observe the effects of allogeneic rabbit serum on the proliferation of rabbit articular chondrocytes cultured in vitro.
METHODS: The chondrocytes were isolated from rabbit knee articular cartilage tissues with 0.4% Pronase and 0.025% collagenase Ⅱ, then equal randomly divided into two groups: the chondrocytes of the experiment group were cultured in Dulbecco's modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10% allogeneic rabbit serum, whereas the culture medium of 10% fetal bovine serum and Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 was used in control group. After subculture, passage 4 cells were obtained.
RESULTS AND CONCLUSIONS: Compared with the control group, the chondrocytes in the experiment group proliferated slowly before the 4th passage. But at the same time, the morphology of chondrocytes in the experiment group was better than that in the control group. When the chondrocytes were at the fourth passage, we found that the shape and growth of chondrocytes in the experiment group achieved better results. Meanwhile the dedifferentiated chondrocytes could be observed in the control group. The 10% allogeneic rabbit serum medium is good for maintaining the shape and proliferation of chondrocytes, so it is indicated that we can get a large number of good chondrocytes through this way.

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