中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1150-1154.doi: 10.3969/j.issn.1673-8225.2011.07.003

• 组织构建与生物力学 tissue construction and biomechanics • 上一篇    下一篇

应力负荷下兔肩袖急性断裂重建后腱-骨修复动物模型建立及其修复过程中影像学评价

李  森1，靳安民2，闵少雄2，张  辉2，王  清1   

  1. 1泸州医学院附属医院脊柱骨科，四川省泸州市646000
    2南方医科大学附属珠江医院骨科中心，广东省广州市 510282
  • 收稿日期:2010-08-07 修回日期:2010-12-03 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 王清，教授，主任医师，硕士生导师。泸州医学院附属医院脊柱外科，四川省泸州市 646000 wqspine2004@ 163.com 并列通讯作者：靳安民，教授，主任医师，博士生导师。南方医科大学珠江医院骨科中心，广东省广州市，510282 Jinanmin2008@163.com
  • 作者简介:李森☆，男，1979年生，汉族，河南省西华县人，2010年南方医科大学毕业，博士，主治医师，主要从事脊柱、脊髓损伤及骨与关节损伤防治的研究。 jht187@163.com
  • 基金资助:

    课题受泸州医学院自然科学基金(泸医院[2010]108号-64)资助。

Construction of repair tendon-bone models in rabbits with postoperatibe acute rotator cuff rupture under stress and imaging evaluation during repairing

Li Sen1, Jin An-min2, Min Shao-xiong2, Zhang Hui2, Wang Qing1   

  1. 1 Department of Spinal Orthopaedics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
    2Orthopedics Center of Zhujiang Hospital, Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2010-08-07 Revised:2010-12-03 Online:2011-02-12 Published:2011-02-12
  • Contact: Wang Qing, Professor, Chief physician, Master’s supervisor, Department of Spinal Orthopaedics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China wqspine2004@163. com Jin An-min, Chief physician, Professor, Doctoral supervisor, Orthopedics Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China Jinanmin2008@163.com
  • About author:Li Sen☆, Doctor, Attending physician, Department of Spinal Orthopaedics, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China jht187@163.com
  • Supported by:

    the Natural Science Foundation of Luzhou Medical College, No. 2010108-64*

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摘要：

背景：目前尚无公认的动物模型可以作为应力负荷下肩袖损伤的相关研究标准的实验动物模型。
目的：建立应力负荷下兔肩袖腱-骨急性断裂重建术后修复动物模型，并对其修复过程中进行影像学评价。
方法：将新西兰白兔进行双侧肩关节行冈上肌腱离断术，并行冈上肌腱止点重建，术后随机分为应力负荷组与非应力负荷组。非应力负荷组正常笼养；应力负荷组于冈上肌腱重建术后第2周开始进行肩关节被动屈伸训练。分别于术后第2，4，8周训练结束后行肩关节MRI和超声检查。
结果与结论：MRI检查结果显示：术后4和8周，T2WI相应力负荷组连接肌腱与骨组织低信号显示面积较非应力负荷组大。超声检查结果显示：术后4和8周，兔冈上肌腱止点重建处连续性回声，应力负荷组较非应力负荷组稍明显。结果提示，应力负荷下兔肩袖急性断裂术后腱-骨修复动物模型建立相对成功，一定的应力刺激能够促进肩袖腱-骨修复。

关键词: 应力负荷, 腱-骨修复, 影像学, 肩袖断裂, 兔, 动物模型

Abstract:

BACKGROUND: Though many kinds of animals were used as models in the experiment of rotator cuff injury under stress, yet there is no single can be as the standard experimental animal model.
OBJECTIVE: To construct repair tendon-bone models of rabbits with acute rotator cuff rupture under stress and to evaluate the iconography in repairing.
METHODS: New Zealand white rabbits were underwent transverse myotenotomy of supraspinatus tendon and tendon insertion site reconstruction. After surgery, all animals were randomly divided into the stress and non-stress groups. Rabbits in the non-stress group were raise in normal cage, and those in the stress group were trained passive flexion and extension at 2 weeks after the reconstruction of the supraspinatus tendon. MRI and ultrasound examination was performed at the 2, 4 and 8 weeks after operation. 
RESULTS AND CONCLUSION: MRI test results: T2WI showed that the areas of the low signals of the tissues connected tendon and bone were larger and the signals of synovial fluid reduced more visible in stress group than in non-stress group at 4 and 8 weeks after operation. Ultrasound examination results: the continuous echoes appeared at the site of the rabbit supraspinatus tendon-bone in 2 groups, which showed more obvious in stress group than in non-stress group at 4 and 8 weeks after operation. The findings demonstrated that, repair tendon-bone models of rabbits with postoperatibe acute rotator cuff rupture under stress are successful constructed, and certain stress can promote repairing of the tendon-bone of rotator cuff.

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