中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1146-1149.doi: 10.3969/j.issn.1673-8225.2011.07.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

D-半乳糖致雄性大鼠的骨丢失

罗红梅1，崔  燎2，吴  铁2，许碧莲2，刘钰瑜2   

  1. 广东医学院，1 生理科学实验室，2药理学教研室，广东省东莞市 523808
  • 收稿日期:2010-09-03 修回日期:2010-10-29 出版日期:2011-02-12 发布日期:2011-02-12
  • 作者简介:罗红梅★，女，1977年生，江西省赣州市人，汉族，2004年广东医学院毕业，硕士，讲师，主要从事骨质疏松方面的研究。 lhmjyb@yahoo.com.cn

D-galactose induced bone loss in male rats

Luo Hong-mei1, Cui Liao2, Wu Tie2, Xu Bi-lian2, Liu Yu-yu2   

  1. 1Laboratory of Physiological Science, 2Department of Pharmacology, Guangdong Medical College, Dongguan  523808, Guangdong Province, China
  • Received:2010-09-03 Revised:2010-10-29 Online:2011-02-12 Published:2011-02-12
  • About author:Luo Hong-mei★, Master, Lecturer, Laboratory of Physiological Science, Guangdong Medical College, Dongguan 523808, Guangdong Province, China lhmjyb@yahoo.com.cn

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330

被引次数

4

摘要：

背景：骨质疏松虽主要受成骨细胞和破骨细胞功能的影响，但D-半乳糖也可导致雄性大鼠骨质丢失。
目的：观察D-半乳糖对大鼠骨组织的影响，并观察其对体外大鼠成骨细胞的影响。
方法：将雄性SD大鼠分成对照组和D-半乳糖组，对照组每天皮下注射生理盐水，D-半乳糖组皮下注5%的D-半乳糖     (100 mg/kg)，连续给药3个月后，取左侧胫骨上端和第四腰椎，塑料包埋切片后，用图像分析仪测量骨动、静态参数。体外培养Wistar大鼠成骨细胞，加入不同浓度的D-半乳糖和阳性药物前列腺素，MTT法测定各组成骨细胞增殖情况，PNPP法测定各组成骨细胞碱性磷酸酶活性。
结果与结论：D-半乳糖组胫骨上端和第四腰椎与对照组相比，骨小梁面积、骨小梁厚度及骨小梁数目降低，骨小梁分离度升高，说明大鼠骨量明显降低，单荧光周长，骨钙化沉积率和骨形成率也降低，说明骨转换率降低。D-半乳糖对体外培养的大鼠成骨细胞数量没有明显影响，但能提高碱性磷酸酶的活性。实验结果说明D-半乳糖可导致低转换型骨丢失，骨丢失不是因为成骨细胞受D-半乳糖抑制直接引起，而是通过其他的间接机制引起。

关键词: 骨丢失, D-半乳糖, 高转换型, 低转换型, 成骨细胞

Abstract:

BACKGROUND: Except osteoblast and osteoclast that can lead to osteoporosis, D-galactose also can result in bone loss in male rats. 
OBJECTIVE: To observe the effect of D-galactose on bone tissue in vivo and osteoblast cultivated in vitro.
METHODS: Male SD rats were divided into control and D-galactose groups. Physiological saline was daily injected into rats in the control group, and 100 mg/kg D-galactose (5%) was administrated for 3 successive months. The left proximal tibia and fourth lumber were harvested for measurement of bone dynamic and static parameters using picture analyzing instrument. Osteoblast was in vitro cultured with different dose D-galactose and prostaglandin, the proliferation of osteoblasts was measured by MTT, and the alkaline phosphatase activity of osteoblast was detected by PNPP way.
RESULTS AND CONCLUSION: Compared with the control group, trabecula area, thichness, and number were decreased, but trabecula separation was increased in the D-galactose group, suggesting that bone mass lose of D-galactose rats. Decreased bone mineralization rate and bone formation rate indicating bone conversion velocity was decreased. D-galactose can induce low conversion rate-induced bone loss, which is not by inhibiting osteoblast directly, but by other indirect ways.

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