中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (44): 8325-8328.doi: 10.3969/j.issn.1673-8225.2011.44.039

• 器官移植临床实践 clinical practice of organ transplantation • 上一篇    下一篇

辐照灭菌冷冻干燥羊膜修复碱烧伤

滕  丹1，栾新尧1，许妮娜1，于丽萍1，李文姬1，刘艳红1，何  伟2   

  1. 1沈阳绿谷生物技术产业有限公司，辽宁省沈阳市，110163
    2沈阳何氏眼科医院，辽宁省沈阳市 110034
  • 收稿日期:2011-06-14 修回日期:2011-09-08 出版日期:2011-10-29 发布日期:2011-10-29
  • 通讯作者: 何伟，博士，主任医师，教授，沈阳何氏眼科医院，辽宁省沈阳市 110034 hewei0111@163.com
  • 作者简介:滕丹★，女，1981年生，2008于辽宁中医药大学毕业，硕士，辽宁省沈阳市人，汉族，主要从事眼科医疗器械的研发工作。 zhenchengsky@tom.com

Transplantation of freeze-dried amnion after irradiation sterilization for corneal alkali burn

Teng Dan1, Luan Xin-yao1, Xu Ni-na1, Yu Li-ping1, Li Wen-ji1, Liu Yan-hong1, He Wei2   

  1. 1Shenyang Green Valley Bio-tech Industry Co.,Ltd., Shenyang  110163, Liaoning Province, China
    2He Eye Hospital, Shenyang  110034, Liaoning Province, China
  • Received:2011-06-14 Revised:2011-09-08 Online:2011-10-29 Published:2011-10-29
  • Contact: He Wei, M.D., Chief physician, Professor, He Eye Hospital, Shenyang 110034, Liaoning Province, China hewei0111@163.com
  • About author:Teng Dan★, Master, Shenyang Green Valley Bio-tech Industry Co.,Ltd., Shenyang 110163, Liaoning Province, China zhenchengsky@tom.com

PDF

454

被引次数

4

摘要：

背景：经辐照灭菌的冷冻干燥羊膜便于保存和使用，但羊膜经这种方式处理后其活性是否会降低、其临床疗效是否会受到影响至今仍鲜有报道。
目的：观察辐照灭菌后的冷冻干燥羊膜对兔角膜碱烧伤的修复作用。
方法：取健康成年白兔9只，均用1 mol/L 氢氧化钠烧伤角膜后，左侧眼设为羊膜组植入辐照灭菌后的冷冻干燥的羊膜，右侧眼设为对照组不植入羊膜。植入后30 d内裂隙灯显微镜每日观察角膜透明度、角膜新生血管及角膜上皮修复情况。
结果与结论：羊膜植入后30 d，羊膜组角膜透明度、新生血管及角膜上皮修复情况明显优于对照组(P < 0.05)，角膜上皮愈合好，基质纤维排列更为整齐，炎细胞浸润程度轻，新生血管更少。结果证实，兔角膜碱烧伤后植入辐照灭菌后的冷冻干燥羊膜可减轻角膜炎性反应，促进角膜上皮修复，减少角膜新生血管形成。

关键词: 冷冻干燥羊膜, 碱烧伤, 辐照灭菌, 角膜, 炎性反应, 组织构建

Abstract:

BACKGROUND: The freeze-dried amnion after radiation sterilization is easy to keep and use. However, whether the amnion’s activity will be reduced and clinical effect will be affected after these treatments have been rarely reported.
OBJECTIVE: To evaluate the repair function of freeze-dried amnion after radiation sterilization on rabbit cornea.
METHODS: Nine healthy adult rabbits were taken and their corneas were burned with 1 mol/L NaOH. The left corneas served as test group and the right corneas as control group. The freeze-dried amnion was planted into the test group but not planted into the control group. Within 30 days after surgery, corneal clearness, neovascularization and recovery of upper cornea were daily observed through the use of slit lamp. 
RESULTS AND CONCLUSION: At 30 days after surgery, corneal clearness, neovascularization and recovery of upper cornea were significantly better in the test group than in the control group (P < 0.05). Compared with the control group, upper cornea was better recovered, matrix fibers were more likely in order, infiltration of inflammatory cells was slighter, and there were fewer newly formed vessels in the test group. These findings suggest that freeze-dried, irradiated amnion can alleviate inflammatory response, promote recovery of upper cornea and reduce neo-vascularization after transplantation into alkali burned rabbit cornea. 

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