中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (49): 9212-.doi: 10.3969/j.issn.1673-8225.2010.49.021

• 干细胞与中医药 • 上一篇    下一篇

黄芪注射液培养大鼠骨髓间充质干细胞的生物活性

孙黎1，焦保良2，王景川2，王新生1   

  1. 1河北北方学院实验中心，河北省张家口市 075029；2河北北方学院第一临床医学院，河北省张家口市  075029
  • 出版日期:2010-12-03 发布日期:2010-12-03
  • 通讯作者: 王新生，博士，教授，硕士生导师，河北北方学院实验中心，河北省张家口市 075029 wxinsheng1968@sina.com
  • 作者简介:孙黎，女，1960年生，河北省徐水县人，汉族，2000年张家口医学院毕业，高级实验师，主要从事细胞生物学研究。 sunli11011@yahoo.com.cn
  • 基金资助:

    河北北方学院博士基金资助项目。

Biological viability of rat bone marrow mesenchymal stem cells after the interference of astragalus mongholicus

Sun Li1, Jiao Bao-liang2, Wang Jing-chuan2, Wang Xin-sheng1   

  1. 1Experimental Center, Hebei North University, Zhangjiakou  075029, Hebei Province, China; 2First Clinical Medical School, Hebei North University, Zhangjiakou  075029, Hebei Province, China
  • Online:2010-12-03 Published:2010-12-03
  • Contact: Wang Xin-sheng, Doctor, Professor, Master’s supervisor, Experimental Center, Hebei North University, Zhangjiakou 075029, Hebei Province, China wxinsheng1968@sina.com
  • About author:Sun Li, Senior laboratory technician, Experimental Center, Hebei North University, Zhangjiakou 075029, Hebei Province, China sunli11011@yahoo.com.cn
  • Supported by:

    the Doctor Foundation of Hebei North University

PDF

378

被引次数

11

摘要：

背景：研究表明，黄芪可促进骨髓间充质干细胞向神经元方向分化。
目的：观察黄芪注射液对大鼠骨髓间充质干细胞活性的影响。
方法：分离、培养SD大鼠骨髓间充质干细胞。取第4代细胞，以50，100，200 g/L的黄芪注射液作用5，12，24 h，MTT实验观测黄芪注射液对大鼠骨髓间充质干细胞活性的影响。
结果与结论：原代培养细胞在8~10 d后接近 80%~90% 融合状态，可进行传代。传至第3代基本完成细胞的纯化。MTT实验显示，伴随黄芪注射液浓度升高和作用时间延长，骨髓间充质干细胞活性逐渐升高，出现类似神经细胞形状改变，其间差异有显著性意义 (P < 0.05)。提示黄芪不仅可以诱导骨髓间充质干细胞分化，并且对细胞具有营养作用。

关键词: 黄芪, 骨髓间充质干细胞, MTT, 活性, 干细胞, 培养与分化

Abstract:

BACKGROUND: Astragalus mongholicus contributes to the differentiation of bone marrow mesenchymal stem cells (BMSCs) into neurons.
OBJECTIVE: To observe effects of astragalus mongholicus on viability of rat BMSCs.
METHODS: BMSCs of Sprague Dawley rats were isolated and cultured. The fourth passage of cells were treated with 50, 100,
200 g/L astragalus mongholicus for 5, 12, 24 hours respectively. BMSCs viability changes were observed by MTT assay after they were induced by astragalus mongholicus.
RESULTS AND CONCLUSION: Primarily cultured cells were closed to 80%-90% fusion status after 8-10 days culture and they could be passaged. The third generation had been purified on the whole. The results from MTT assay showed that the BMSCs viability would be increased with the increased concentration of astragalus mongholicus and the prolonged action time. Morphological changes in neural cells appeared. There were significant differences between groups (P < 0.05). These indicate that astragalus mongholicus can induce the differentiation of BMSCs and nourish cells.

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