中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (32): 5173-5178.doi: 10.3969/j.issn.2095-4344.2867

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

对比组织悬液法及组织块法建立兔VX2皮下肿瘤模型的差异

刘  娅，冯  爽，益  磋，余堰澜，刘  政   

  1. 陆军军医大学附属新桥医院超声科，重庆市   400037

  • 收稿日期:2019-10-28 修回日期:2019-10-31 接受日期:2019-12-13 出版日期:2020-11-18 发布日期:2020-09-25
  • 通讯作者: 刘政，博士，博士生导师，陆军军医大学附属新桥医院超声科，重庆市 400037
  • 作者简介:刘娅，女，1989年生，重庆市人，汉族，2010年陆军军医大学毕业，主治医师，主要从事成人及小儿腹部、心脏及小器官超声诊断研究。
  • 基金资助:
    国家重点研发计划项目(2017YFC0107300)

Comparing tumor suspension injection and tissue explant to establish rabbit VX2 subcutaneous tumor model

Liu Ya, Feng Shuang, Yi Cuo, Yu Yanlan, Liu Zheng   

  1. Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing 400037, China
  • Received:2019-10-28 Revised:2019-10-31 Accepted:2019-12-13 Online:2020-11-18 Published:2020-09-25
  • Contact: Liu Zheng, MD, Doctoral supervisor, Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing 400037, China
  • About author:Liu Ya, Attending physician, Department of Ultrasound, Xinqiao Hospital, Army Medical University, Chongqing 400037, China
  • Supported by:
    the National Key Research and Development Project of China, No. 2017YFC0107300

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摘要：

文题释义：

彩色多普勒血流显像(Color Doppler Flow Imaging，CDFI)：是利用红细胞与超声波之间的多普勒原理进行血流显像的技术，根据血流的方向和速度大小，进行伪彩色编码，后经彩色显示器显示，从而完成彩色多普勒血流显像。

VX2肿瘤(VX2 Tumor)：来源于Shope病毒，属于鳞状细胞癌的一种，可使乳头瘤恶变成鳞状上皮细胞癌的恶性肿瘤。因具有与人类肿瘤相似的形态学及生物学特性，目前主要用于动物模型的实验研究，尤其是兔VX2各组织器官肿瘤的影像学及肿瘤治疗的动物模型研究。

背景：目前建立VX2皮下肿瘤模型的方法有多种，但建模方法各有优劣，能优质高效建立动物肿瘤模型的方法方可满足动物肿瘤实验的需求。

目的：对比观察不同方法建立兔VX2皮下肿瘤模型，探索更简单高效的建模方式，为兔VX2皮下肿瘤实验提供大批量高质量的动物模型奠定基础。

方法：雄性新西兰大白兔66只。取2只制备VX2荷瘤种兔，再取荷瘤兔肿瘤组织分别制备瘤组织块及肿瘤组织悬液备用。实验分为2组：瘤组织悬液组20只，麻醉后于双侧后肢内侧面注射组织悬液0.15 mL；瘤组织块组20只，麻醉后于双侧后肢内侧面皮下植入瘤组织块。2组分别采用相应接种方式种植2只荷瘤种兔，各传5代。记录并比较2组肿瘤种植时间，采用二维及彩色多普勒超声观察肿瘤形态大小及生长情况，比较2组肿瘤成瘤率及传代情况。实验方案经中国人民解放军第三军医大学动物实验伦理委员会批准(批准号为AMUWE2020016)。

结果与结论：①组织悬液法肿瘤种植时间(75.70±11.16) s较组织块法肿瘤种植时间(100.80±9.21) s明显缩短(P=0.00)；②组织悬液法成瘤率95%显著高于组织块法成瘤率60%(P < 0.05)，肿瘤体积显著大于组织块法(P < 0.05)；③组织悬液法传代成功率95%显著高于组织块法传代成功率65%(P < 0.05)；④结果说明，组织悬液法建立兔VX2皮下肿瘤模型与瘤块法相比，更简单高效。

ORCID: 0000-0002-1281-4437(刘娅)

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织悬液, 组织块, 兔, VX2, 肿瘤模型, 超声, 成瘤率

Abstract:

BACKGROUND: There are many methods to establish VX2 subcutaneous tumor model, and the existing modeling methods have their own advantages and disadvantages. To meet the needs of tumor experiments on animals, the method of establishing an animal tumor model with high-quality and high-efficiency is necessary.

OBJECTIVE: To explore more simple and efficient way of modeling, provide rabbit VX2 subcutaneous tumor

experiment with large quantities of high-quality animal models by comparing different methods to establish rabbit VX2 subcutaneous tumor model.

METHODS: Sixty-six male New Zealand white rabbits were enrolled. Two of the 66 rabbits were used to prepare VX2 tumor-bearing rabbits, and tumor tissues were taken from the tumor-bearing rabbits to prepare tumor tissue blocks and tumor tissue suspensions. There were two groups in the experiment. In tumor tissue suspension group (n=20), the rabbits were injected with 0.15 mL of tissue suspension on the medial side of bilateral hind limbs after anesthesia; in tumor tissue block group (n=20), tumor tissue blocks were implanted subcutaneously on the medial side of bilateral hind limbs after anesthesia. Two tumor-bearing rabbits from each group were subjected to the corresponding vaccination methods, each passed for five generations. Tumor inoculation time in the two groups was record and compared. The tumor size and growth were observed by ultrasound with 2-D and CDFI mode. Tumor-take rate and serial passage of tumor tissues were observed and compared between the two groups. The experimental protocol was approved by the Animal Experiment Ethics Committee of the Army Medical University of PLA (approval No. AMUWE2020016)

RESULTS AND CONCLUSION: The tumor inoculation time in the tissue suspension group [(75.70±11.16) s] was significant shortened compared with that in the tissue block group [(100.80±9.21) s; P=0.00]. The tumor-take rate was significantly higher in the tissue suspension group than the tissue block group (95% vs. 60%; P < 0.05). The tumor size was significantly larger in the tissue suspension group than the tissue block group (P < 0.05). The rate of tumor tissue series passage was significantly higher in the tissue suspension group than the tissue block group (95% vs. 65%; P < 0.05). Therefore, tissue suspension method for making the model of rabbit VX2 subcutaneous tumor is simpler and more efficient compared with the tissue block method.

Key words: tumor suspension, tissue block, rabbit, VX2, tumor model, ultrasound, tumor-take rate

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