中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (8): 1241-1245.doi: 10.3969/j.issn.2095-4344.2017.08.016

• 骨组织构建 bone tissue construction • 上一篇    下一篇

不同艾灸距离对Ⅱ型胶原诱导型关节炎疗效的影响

王  洋,吴心雨,孙志岭,张永怡,薛  莲,秦  雪   

  1. 南京中医药大学护理学院,江苏省南京市  210023
  • 收稿日期:2017-01-20 出版日期:2017-03-18 发布日期:2017-04-14
  • 通讯作者: 孙志岭,博士,教授,南京中医药大学护理学院,江苏省南京市 210023
  • 作者简介:王洋,女,1990年生,江苏省泰州市人,汉族,南京中医药大学在读硕士。
  • 基金资助:

    江苏省“青蓝工程”资助项目(2014);江苏省普通高校学术学位研究生科研创新计划项目(KYLX16_1164);江苏省高等学校大学生创新创业训练计划重点项目(201610315015Z);江苏省优势学科二期项目南京中医药大学护理学一级学科指令性课题(YSHL2016-014)

Therapeutic efficacy of moxibustion at different distances on type II collagen-induced arthritis

Wang Yang, Wu Xin-yu, Sun Zhi-ling, Zhang Yong-yi, Xue Lian, Qin Xue   

  1. School of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
  • Received:2017-01-20 Online:2017-03-18 Published:2017-04-14
  • Contact: Sun Zhi-ling, M.D., Professor, School of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
  • About author:Wang Yang, Studying for master’s degree, School of Nursing, Nanjing University of Chinese Medicine, Nanjing 210023, Jiangsu Province, China
  • Supported by:

    the “Blue Engineering” of Jiangsu Province in 2014; the Academic Postgraduate Research Innovation Program of Colleges of Jiangsu Province, No. KYLX16-1164; the Key Project of the Student Innovative Entrepreneurship Training Program in Universities of Jiangsu Province, No. 201610315015Z; the Jiangsu Provincial Dominant Subject Second Stage Project of the Nursing Class I Instructive Subject of Nanjing University of Chinese Medicine, No. YSHL2016-014

摘要:

文章快速阅读:


文题释义:
类风湿关节炎的艾灸治疗:规范、安全的应用艾灸治疗类风湿关节炎可以促进艾灸疗法在临床的推广应用。已往众多研究表明了艾灸治疗类风湿关节炎具有抗炎、镇痛的疗效,课题组已从蛋白组学角度验证了艾灸治疗类风湿关节炎的有效性。
摘要
背景:
有研究表明艾灸能够改善类风湿关节炎的关节症状、降低炎症反应,但艾灸操作缺乏标准。艾灸距离成为影响艾灸疗效的因素之一。
目的:对比不同艾灸距离治疗类风湿关节炎的疗效差异,探索最佳灸距。
方法:将40只雄性SD大鼠随机分出8只作为对照组,其他32只均造模处理。建立Ⅱ型胶原诱导型关节炎模型大鼠,分别按不同艾灸距离1,2,3 cm灸“肾俞(BL23)”及“足三里(ST36)”穴,每日灸1次,每个穴位10 min,每个疗程为6 d,各疗程间休息1 d,总共干预3个疗程,同时设置模型组。
结果与结论:①一般情况及炎性反应情况:模型组大鼠除体质量外,足趾容积、关节炎评分、血清白细胞介素1β、肿瘤坏死因子α水平较对照组显著升高(P < 0.01),艾灸干预后上述各指标艾灸各组较模型组均明显降低(P < 0.01),并且艾灸1,2 cm组优于艾灸3 cm组(P < 0.01);②踝关节组织学变化:模型组可见关节腔变窄、关节腔内可见炎细胞及血管翳形成,艾灸1,2,3 cm组可见中度炎细胞浸润,血管翳较少;③结果说明,艾灸在改善关节功能、调节免疫方面具有一定作用,可下调白细胞介素1β、肿瘤坏死因子α的水平,减轻滑膜增生。艾灸距离为1或2 cm时,可提高艾灸的效果。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0001-8108-7598(王洋)

关键词: 组织工程, 骨组织工程, 艾灸, 距离, 胶原诱导型关节炎, 类风湿关节炎, 细胞因子, 白细胞介素1β, 肿瘤坏死因子α, 关节炎指数, 大鼠

Abstract:

BACKGROUND: Moxibustion can improve the symptoms of rheumatoid arthritis and reduce inflammation, but there are no uniform operation standards. The moxibustion distance becomes one of factor influencing the therapeutic efficacy.
OBJECTIVE: To compare the effects of different moxibustion distances on rheumatoid arthritis, and to explore the optimal distance.
METHODS: The 8 of 40 male Sprague-Dawley rats were randomly selected as controls. The other 32 were used to make animal modes of collagen type II-induced ankle arthritis, and then given moxibustion at Shenshu (BL23) and Zusanli (ST36) at an interval of 1, 2 and 3 cm, respectively, once daily, 10 minutes at each point, 6 days per course for three courses, with a course interval of 1 day. Model rats with no treatment acted as model group.
RESULTS AND CONCLUSION: Compared with the control group, the toe volume, arthritis index and serum levels of interleukin-1β and tumor necrosis factor-α except the body mass were significantly increased in the model group (P < 0.01). After moxibustion treatment, these indexes were significantly decreased in the three treatment groups (P < 0.01), especially in the 1 and 2 cm groups (P < 0.01). Narrowed articular cavity, intra-articular inflammation and pannus formation were observed in the model group, while only moderate inflammatory cell infiltration and few pannus formation were found in the 1, 2, 3 cm groups. These results indicate that moxibustion improves joint functions and regulates immune reaction by downregulating the levels of interleukin-1β and tumor necrosis factor-α, as well as reducing synovial hyperplasia. In addition, the optimal distance for moxibustion is 1 or 2 cm, which is a momentous proposition to improve therapeutic efficacy.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Tissue Engineering, Arthritis, Experimental, Arthritis, Rheumatoid, Rats

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