中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (8): 1242-1246.doi: 10.12307/2022.230

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

高剂量维生素C促进大鼠自体脂肪移植成活

项鑫健,柳  芳,吴亮亮,贾大平,陶  越,赵政男,赵  宇   

  1. 安徽医科大学第一附属医院整形外科,安徽省合肥市   230022
  • 收稿日期:2020-11-04 修回日期:2020-11-05 接受日期:2020-12-14 出版日期:2022-03-18 发布日期:2021-11-02
  • 通讯作者: 赵宇,博士,主任医师,安徽医科大学第一附属医院整形外科,安徽省合肥市 230022
  • 作者简介:项鑫健,男,1996年生,安徽省太和县人,汉族,安徽医科大学在读硕士,主要从事生物组织工程、材料学及炎性反应的研究。
  • 基金资助:
    国家自然科学基金(81171829),项目负责人:赵宇;安徽省转化医学研究院科研基金(2017zhuyx05),项目负责人:赵宇

High-dose vitamin C promotes the survival of autologous fat transplantation in rats

Xiang Xinjian, Liu Fang, Wu Liangliang, Jia Daping, Tao Yue, Zhao Zhengnan, Zhao Yu   

  1. Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
  • Received:2020-11-04 Revised:2020-11-05 Accepted:2020-12-14 Online:2022-03-18 Published:2021-11-02
  • Contact: Zhao Yu, MD, Chief physician, Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
  • About author:Xiang Xinjian, Master candidate, Department of Plastic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81171829 (to ZY); Scientific Research Fund of Anhui Translational Medicine Research Institute, No. 2017zhuyx05 (to ZY)

摘要:

文题释义:
自体脂肪移植:选取脂肪富余部位为供区,从供区获得脂肪进行纯化处理,然后移植到自体欠缺脂肪的区域,以达到再造、美容等目的。供区通常选择腹部、大腿内侧等隐蔽部位,少数也可选择网膜等内脏脂肪。临床上获得颗粒脂肪常通过肿胀麻醉法将供区局部麻醉,再通过抽脂针连接负压抽吸;纯化方法通常选择吸附、静置或离心。
维生素C:又称抗坏血酸,是一种多羟基化合物,化学式为C6H8O6,具有很强的还原性,其分子易解离而释出H+,故呈酸性。维生素C是临床及科研上广泛使用的抗氧化剂。在生物医学中,维生素C具备有参与组织合成代谢、抗氧化、抗自由基、改善免疫功能、保护血管内皮、改善器官功能障碍等作用。
背景:脂肪移植常应用于软组织填充、再造、重建及美容等,但脂肪移植的成活率差异较大,寻找提高脂肪移植成活率的方法成为研究重点。
目的:探究自体脂肪移植后给予不同剂量维生素C对脂肪移植成活的影响。
方法: 20只雌性SD大鼠,随机分成低剂量维生素C组、高剂量维生素C组、超高剂量维生素C组及对照组,每组5只。取出各组大鼠腹腔肠系膜脂肪,适当处理后取0.8 g颗粒脂肪移植于背部皮下直径1 cm囊袋中,术后连续7 d前3组分别腹腔注射50,150,1 000 mg/d的维生素C溶液1 mL,对照组腹腔注射等量生理盐水。移植后8周将大鼠安乐死,取出移植物,剥除粘连纤维结缔组织后进行大体观察、称移植物留存质量、苏木精-伊红染色、CD34免疫组织化学染色。
结果与结论:①3个维生素C组的脂肪质地较对照组柔软,表面有更多的细小血管,脂肪移植物质量均大于对照组,且以高剂量维生素C组效果最明显(P < 0.001);②3个维生素C组均可减少囊泡形成,高剂量维生素C组、超高剂量维生素C组在减轻炎性浸润及纤维化上更显著(P < 0.05);③高剂量维生素C组、超高剂量维生素C组的毛细血管密度较大,均高于对照组(P < 0.001);④结果表明,高剂量维生素C可以提高大鼠自体脂肪的毛细血管密度及留存质量,减轻炎性浸润及纤维化程度,减少囊泡形成。

https://orcid.org/0000-0001-6621-9927 (项鑫健) 

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程

关键词: 维生素C, 自体脂肪移植, 抗氧化, 移植物吸收, 炎症, 纤维化, 最适剂量, 大鼠

Abstract: BACKGROUND: Fat transplantation is often used for soft tissue filling, reconstruction, and beauty treatments. However, the survival rate of fat transplants varies greatly. How to improve the survival rate of fat transplants has become a research focus.
OBJECTIVE: To explore the effect of different doses of vitamin C on the survival of fat transplants after autologous fat transplantation.  
METHODS: Twenty female Sprague-Dawley rats were randomized into a low-, high-, super-high-dose vitamin C groups and control group (n=5 per group). The abdominal mesenteric fat of rats in each group was taken out, and 0.8 g of fat particles was transplanted into a 1 cm diameter subcutaneous sac on the back of each rat after proper treatment. The experimental groups were intraperitoneally injected with 1 mL of vitamin C solution of 50, 150, and 1 000 mg/d respectively for 7 consecutive days after operation. The control group was intraperitoneally injected with the same amount of normal saline. After 8 weeks, the rats were euthanized. Then the grafts were taken out and evaluated as follows after removal of the adherent fibrous connective tissue: (1) gross observation; (2) retention weight; (3) hematoxylin-eosin staining; (4) immunohistochemical staining of CD34.
RESULTS AND CONCLUSION: The texture of fat in the three vitamin C groups was generally softer than that of the control group, and there were more small blood vessels on the surface of the fat graft. The graft retention weight of the vitamin C groups was significantly higher than that of the control group, and the retention weight of the high-dose group was highest (P < 0.001). The formation of vesicles in the three vitamin C groups was reduced. High- and super-high-dose vitamin C was more effective in reducing inflammatory infiltration and fibrosis (P < 0.05). The capillary densities in the high- and super-high-dose vitamin C groups were higher than that in the control group (P < 0.001). To conclude, high-dose vitamin C can increase the capillary density and retention quality of autologous fat, reduce the degree of inflammatory infiltration and fibrosis, and reduce the formation of vesicles in rats.

Key words: vitamin C, autologous fat transplantation, antioxidant, absorption of autograft, inflammation, fibrosis, optimal dosage, rat

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