中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (50): 7530-7535.doi: 10.3969/j.issn.2095-4344.2016.50.012

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

复合骨髓间充质干细胞生物学特性及修复皮肤缺损

杜  鹏，王晓川，关  真   

  1. 昆明理工大学附属医院(云南省第一人民医院)，云南省昆明市  650001
  • 修回日期:2016-10-20 出版日期:2016-12-02 发布日期:2016-12-02
  • 通讯作者: 关真，在读硕士，主管护师，昆明理工大学附属医院(云南省第一人民医院)，云南省昆明市 650001
  • 作者简介:杜鹏，男，1974年生，山东省济南市人，2003年昆明医学院毕业，硕士，主治医师，主要从事皮肤病与性病研究。 并列第一作者：王晓川，女，1978年生，云南省个旧市人，汉族，2012年昆明医学院毕业，硕士，主治医师，主要从事皮肤病与性病研究。
  • 基金资助:

    云南省教育厅科学研究院基金项目(20144C004Y)

Biological characteristics of bone marrow mesenchymal stem cells and application in skin defect repair

Du Peng, Wang Xiao-chuan, Guan Zhen   

  1. First People’s Hospital of Yunnan Province, Kunming 650001, Yunnan Province, China
  • Revised:2016-10-20 Online:2016-12-02 Published:2016-12-02
  • Contact: Guan Zhen, Studying for master’s degree, Nurse in charge, First People’s Hospital of Yunnan Province, Kunming 650001, Yunnan Province, China
  • About author:Du Peng, Master, Attending physician, First People’s Hospital of Yunnan Province, Kunming 650001, Yunnan Province, China Wang Xiao-chuan, Master, Attending physician, First People’s Hospital of Yunnan Province, Kunming 650001, Yunnan Province, China Du Peng and Wang Xiao-chuan contributed equally to this work.
  • Supported by:

    the grant from the Academy of Science of Yunnan Educational Department, No. 20144C004Y

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被引次数

2

摘要：

文章快速阅读：

文题释义：
复合骨髓间充质干细胞：一类特殊的骨髓间充质干细胞，该细胞含有组织特性多能干细胞，该细胞在特定的条件下能分化为骨、软骨、脂肪等细胞，细胞具有来源丰富、分离培养容易、增殖迅速、明显的可塑性、免疫原性较弱，能转染外基因等特点，具有广泛的应用前景的移植用细胞，也是抑制治疗和构建人工组织或器官理想的种子细胞。
干细胞的生物学特性：干细胞具有高度自我更新能力、高度繁殖和多向分化潜能，界定干细胞，有4条标准：①细胞可进行多次的，连续的，自我更新式的细胞分裂，这是维持群体稳定的首要条件；②起源于单一细胞的种子细胞可分化超过1种以上的细胞类型，例如造血干细胞可分化为所有的血细胞，有些成熟干细胞只能分化成单一的细胞类型，例如角膜干细胞；③当干细胞被移植入损伤的患者体内时有重建原来组织的功能。④不易确定的标准：即使无组织损伤，干细胞也能在体内分化扩增，胚胎干细胞能完全符合上述标准，能以一种不确定的未分化状态扩增，将其注入胚泡中，便能生成所有类型的细胞。

 

摘要
背景：组织工程皮肤是利用来源于肢体或异体的皮肤细胞，选择合适的生物材料直接进行复合，在体外构建皮肤类似物，并将其移植到缺损的皮肤创面，加速缺损皮肤修复，能巧妙的解决皮肤不足等问题。
目的：分析复合骨髓间充质干细胞生物学特性，探讨复合骨髓间充质干细胞在皮肤缺损修复中的应用效果。
方法：28只SPF级SD大鼠，随机分为2组，每组14只。调节温度、时间、压力以及面积的数控电烫仪，建立大鼠皮肤烫伤缺损模型，复合骨髓间充质干细胞组采用复合骨髓间充质干细胞进行修复，凡士林油修复组采用凡士林纱布覆盖进行修复，比较两组大鼠修复效果。
结果与结论：①复合骨髓间充质干细胞培养72 h后细胞分散分布，外形为小圆形；培养5 d后细胞开始伸展，细胞形态不稳定，传代第3代后细胞形态趋于稳定。抗原鉴定显示：复合骨髓间充质干细胞中存在CD44以及CD29表达，CD45以及CD34表达较低；②复合骨髓间充质干细胞组大鼠修复28 d后创面基本愈合并未见明显瘢痕；凡士林油修复组修复28 d后创面愈合，但是创面存在创面存在少量的收缩，瘢痕明显；凡士林油修复组创面处皮肤表皮相对较厚，基底层与真皮层交接局部连接效果欠佳。复合骨髓间充质干细胞组表皮细胞分层明显，排列相对比较整齐，且表皮与真皮连接比较紧密；③结果提示：分离鉴定的细胞为复合骨髓间充质干细胞，较容易获得。该细胞修复皮肤缺损效果理想，能促进皮肤缺损部位的早期愈合，改善缺损部位血液循环，具有较高的临床应用价值。

 

 

关键词: 干细胞, 移植, 复合骨髓间充质干细胞, 生物学特性, 皮肤缺损, 修复, 应用效果, 数控电烫仪, 烫伤缺损, 修复效果, 凡士林油

Abstract:

BACKGROUND: An appropriate biomaterial can be directly combined with autologous or allogeneic skin cells to construct tissue-engineered skin, which can accelerate skin repair after transplantation onto the skin wounds. It is a good idea to solve the deficiency in skin sources.
OBJECTIVE: To study the biological characteristics of the complex with bone marrow mesenchymal stem cells and to investigate the application of this complex in skin defect repair.
METHODS: Twenty-eight Sprague-Dawley rats, SPF grade, were randomized into two groups (n=14 per group). By adjustment of temperature, time, pressure and area of NC perm instrument, scald models were made in rats. Rats in the treatment group were given the repair using composite bone marrow mesenchymal stem cells, while those in the control group were given vaseline gauze repair. Repair effects were compared between two groups.
RESULTS AND CONCLUSION: (1) After 72 hours of culture, the composite bone marrow mesenchymal stem cells small with round shape distributed dispersedly. After 5 days of culture, the cells began to stretch and the cell morphology became unstable. After three passages, the cell morphology became stable. The results of antigen identification showed the expression of CD44 and CD29 but the low expression of CD45 and CD34 in bone marrow mesenchymal stem cells. (2) Twenty-eight days after repair, there was no obvious scar on the wound surface of the treatment group, but a little shrinkage and obvious scar still existed in the control group. Moreover, in the control group, the epidermal layer of the skin was relatively thick, and the connection between the basal layer and the dermis was unsatisfactory. In the treatment group, obvious epidermal cell stratification, neat arrangement, and tight connection between the epidermis and dermis were observed. In summary, bone marrow mesenchymal stem cells are a special class of cells that have pluripotent ability and are more readily available. These cells are the preferred target cells for skin defect repair to promote early healing of the skin and improve blood circulation defect site, which are confirmed to have high clinical value.

 

 

Key words: Stem Cells, Skin, Artificial, Biocompatible Materials, Tissue Engineering

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