中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (49): 7431-7436.doi: 10.3969/j.issn.2095-4344.2016.49.020

• 口腔损伤及修复动物模型 Animal models of oral lesions and repair • 上一篇    下一篇

实验性根尖周炎模型大鼠核因子?B的表达

叶丹丹,王婷婷,董  明,金海威,牛卫东   

  1. 大连医科大学口腔医学院口腔内科,辽宁省大连市  116044
  • 收稿日期:2016-09-06 出版日期:2016-11-30 发布日期:2016-11-30
  • 通讯作者: 牛卫东,博士,教授,大连医科大学口腔医学院口腔内科,辽宁省大连市 116044
  • 作者简介:叶丹丹,女,1990年生,安徽省青草镇人,汉族,大连医科大学在读硕士,主要从事口腔内科的研究。
  • 基金资助:

    国家自然科学基金项目(81171538)

Expression of nuclear factor-kappa B in rat models of periapical periodontitis

Ye Dan-dan, Wang Ting-ting, Dong Ming, Jin Hai-wei, Niu Wei-dong   

  1. Department of Oral Medicine, School of Stomatology of Dalian Medical University, Dalian 116044, Liaoning Province, China
  • Received:2016-09-06 Online:2016-11-30 Published:2016-11-30
  • Contact: Niu Wei-dong, M.D., Professor, Department of Oral Medicine, School of Stomatology of Dalian Medical University, Dalian 116044, Liaoning Province, China
  • About author:Ye Dan-dan, Studying for master’s degree, Department of Oral Medicine, School of Stomatology of Dalian Medical University, Dalian 116044, Liaoning Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81171538

摘要:

文章快速阅读:


文题释义:
根尖周炎:是指发生于根尖周围组织的炎症性疾病,又称根尖周病,多为牙髓病的继发病,主要由根管内的感染通过根尖孔作用于根尖周组织引发炎症反应,同时引起骨破坏的一类疾病。
NF-?B:作为一种分布和作用均十分广泛的真核细胞转录因子,NF-κB的激活不需要新翻译出的蛋白进行调控。因此,它可以在第一时间对有害细胞的刺激做出反应。大多数的细菌可以结合细胞膜表面的受体,从而激发NF-κB信号通路改变基因的表达。
摘要
背景:
研究指出核因子?B在人根尖周炎组织中mRNA的表达水平明显高于正常根尖周组织。
目的:观察核因子?B在大鼠实验性根尖周炎部位的表达。
方法:取6周龄雄性SD 大鼠 20只,右侧下颌第1磨牙建立根尖周炎模型为实验组;左侧下颌第1磨牙为正常对照组。分别于术后 1,2,3,4 周随机取 5 只鼠,取下颌骨组织块制作冰冻切片。苏木精-伊红染色观察根尖区组织炎症的病理特征,免疫组织化学染色检测核因子?B 在大鼠根尖周炎组织中的表达与分布。
结果与结论:①苏木精-伊红染色见实验侧下颌第1磨牙根尖周组织炎性浸润,向周围牙槽骨扩散蔓延;正常对照组未见病理表现;说明建模成功;②免疫组织化学染色显示,造模后 1,2,3,4 周,实验组的根尖周炎症区域中均可见核因子?B呈阳性表达。从 1 周至 2 周其表达量增加,2 周时核因子?B 的表达量呈现高峰,3 周开始降低,4 周时最低,各个时间点的表达差异均有显著性意义(P < 0.05);实验组的阳性细胞数均高于正常对照组(P < 0.05);③结果表明,核因子?B 的表达情况与根尖周炎的进程密切相关,提示核因子?B 参与了根尖周炎的炎症反应过程。

中国组织工程研究杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程
ORCID: 0000-0003-1778-6209(叶丹丹)

关键词: 实验动物, 口腔损伤与修复动物模型, 根尖周炎, NF-?B, 大鼠, 炎症模型, 免疫组化, 骨吸收, 核转录因子, 国家自然科学基金

Abstract:

BACKGROUND: mRNA expression level of nuclear factor-kappa B (NF-?B) in periapical periodontitis has been shown to be significantly higher than that in normal periapical tissue.
OBJECTIVE: To observe the expression of NF-?B in rats with periapical periodontitis.
METHODS: Twenty male Sprague-Dawley rats aged 6 weeks were enrolled to establish apical periodontitis model at the right mandibular first motar (experimental group), and the left mandibular first motar as normal control group. Five rats were randomly selected at postoperative 1, 2, 3, and 4 weeks to make frozen sections of the mandibular tissue. The morphology of the periapical tissue was observed through hematoxylin-eosin staining, and the expression level and distribution of NF-?B were detected by immunohistochemical staining.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed that the apical periodontal tissue of the mandibular first molar at the right side had local inflammatory response and spread to peripheral tissues; while there was no pathological changes in the control group. (2) Immunohistochemistry results revealed that NF-?B expression increased from the 1st week to 2nd week after surgery, and then went down at the 3rd week and reached the lowest point at the 4th week; moreover, the expression level at each time exhibited significant differences (P < 0.05). The amount of NF-?B-positive cells in the experimental group was significantly higher than that in the control group (P < 0.05). (3) These findings suggest that the expression level of NF-?B holds close correlation with the process of periapical periodontitis indicating that NF-?B may participate in the inflammatory reaction of periapical periodontitis. 

中国组织工程研究杂志出版内容重点:肾移植肝移植移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植组织工程

Key words: NF-kappa B, Tooth, Periodontitis, Tissue Engineering

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