中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (3): 370-376.doi: 10.3969/j.issn.2095-4344.2016.03.012

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

牙科氧化锆氧化铝陶瓷复合材料的生物相容性

邓庶男1，黄红燕1，战德松2，梁在赋3   

  1. 1沈阳市口腔医院，辽宁省沈阳市  110002；2中国医科大学口腔医学院，辽宁省沈阳市  110002；3中国医科大学实验技术中心，辽宁省沈阳市  110001
  • 收稿日期:2015-12-12 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: 黄红燕，沈阳市口腔医院，辽宁省沈阳市 110002
  • 作者简介:邓庶男，男，1973年生，辽宁省沈阳市人，汉族，1996年锦州医学院毕业，主要从事修复材料与工艺研究。
  • 基金资助:
    沈阳科技计划资助项目(1071170-9-01)

Biocompatibility of ZrO2 and Al2O3 ceramic composites in dentistry

Deng Shu-nan1, Huang Hong-yan1, Zhan De-song2, Liang Zai-fu3   

  1. 1Stomatology Hospital of Shenyang, Shenyang 110002, Liaoning Province, China; 2School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China; 3Experimental Technology Center, China Medical University, Shenyang 110001, Liaoning Province, China
  • Received:2015-12-12 Online:2016-01-15 Published:2016-01-15
  • Contact: Huang Hong-yan, Stomatology Hospital of Shenyang, Shenyang 110002, Liaoning Province, China
  • About author:Deng Shu-nan, Stomatology Hospital of Shenyang, Shenyang 110002, Liaoning Province, China
  • Supported by:

    the Science and Technology Projects of Shenyang, China, No. 1071170-9-01

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394

被引次数

5

摘要：

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文题释义：

氧化锆氧化铝复合陶瓷的最佳配比：有关研究表明，氧化锆氧化铝复合陶瓷具有良好的生物相容性和优良的综合力学性能，已成为口腔全瓷修复的研究热点和发展方向。但氧化锆氧化铝复合陶瓷的具体配比始终存在争议。课题组前期采用溶胶共沉淀法辅以共沸蒸馏工艺，制备出了具有较高烧结活性的牙科用氧化锆氧化铝复合超细粉体，探讨了不同配比(1∶1；4∶1)氧化锆氧化铝复合粉体对复合粉体晶型晶貌的影响。此次实验对研制出的不同配比(1∶1；4∶1)的氧化锆氧化铝复合材料进行急性溶血试验、细胞毒性试验，初步评价其生物相容性，以期为更好的应用于临床提供理论依据。 

关键词: 生物材料, 口腔生物材料, 生物相容性, 氧化锆, 氧化铝, 牙科复合陶瓷, 体外细胞毒实验, 急性溶血性实验, 浸提液, 四唑盐比色法, 生长曲线

Abstract:

BACKGROUND: The physiological environment of oral cavity is very complex. Dental restoration materials exposed to the weakly acidic body fluids in the long term, and were in the complex influences including physical, chemical, biological and mechanical and other factors. Therefore, the evaluation of biocompatibility for dental materials is the focus of research before entering the clinical trials, and also the important technical indicators to 
ensure the clinical safety.
OBJECTIVE: To preliminarily evaluate the biocompatibility of ZrO2 and Al2O3 ceramic composites.
METHODS: The experiment was divided into six groups: ZrO2 and Al2O3 (1:1) 100% extract group, ZrO2 and Al2O3 (1:1) 50% extract group, ZrO2 and Al2O3 ( 4:1) 100% extract group, ZrO2 and Al2O3 (4:1) 50% extract group, and the volume fraction of 0.64% phenol positive control group and 100% fresh medium of negative control group. Acute hemolysis and in vitro cytotoxicity experiments were conducted according to the biological evaluation standards and requirements of GB-T16886 15-2003 medical devices. The effects of the above six groups of extracts on rabbit blood cells and L-929 mouse fibroblast cells were tested respectively.
RESULTS AND CONCLUSION: There was no significant difference in the absorbance values between different concentrations of experimental groups and negative control group (P > 0.05). The cytotoxicity rating of all experimental groups was 0-1 grade. The hemolysis rates in the ZrO2 and Al2O3 (1:1) 100% extract and ZrO2 and Al2O3 (4:1) 100% extract groups were 1.27% and 2.4% respectively, less than the accepted safety standards of 5%. These results demonstrate that the acute hemolytic experiment and in vitro cytotoxicity experiment of (1:1), (4:1) ZrO2 and Al2O3 ceramic composites are all negative, and all have good cytocompatibility and blood compatibility.