中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (49): 7878-7878.doi: 10.3969/j.issn.2095-4344.2015.49.001

• 周围神经损伤动物模型 Animal models of peripheral nerve injury •    下一篇

温针对腰神经根受压模型大鼠组织形态的影响

谢艳艳1，吴耀持2   

  1. 1上海市第六人民医院东院针推伤科，上海市 201306；2上海交通大学附属第六人民医院针推伤科，上海市 200233
  • 收稿日期:2015-09-07 出版日期:2015-11-30 发布日期:2015-11-30
  • 通讯作者: 吴耀持，教授，博士生导师，主任医师，上海交通大学附属第六人民医院针推伤科，上海市 200233
  • 作者简介:谢艳艳，女，1981年生，河南省济源市人，汉族，2007年上海中医药大学毕业，硕士，主治医师，主要从事电针治疗腰椎间盘突出症的研究。
  • 基金资助:

    上海市科委资助课题(04419723)：从细胞因子调控探讨温和灸对大鼠腰神经根受压模型的作用机制

Effect of warm needling on histomorphology of compressed lumbar nerve root in rats

Xie Yan-yan1, Wu Yao-chi2   

  1. 1Department of Acupuncture Traumatology, Eastern Hospital of Shanghai Sixth People’s Hospital, Shanghai 201306, China; 2Department of Acupuncture Traumatology, the Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • Received:2015-09-07 Online:2015-11-30 Published:2015-11-30
  • Contact: Wu Yao-chi, Professor, Doctoral supervisor, Chief physician, Department of Acupuncture Traumatology, the Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China
  • About author:Xie Yan-yan, Master, Attending physician, Department of Acupuncture Traumatology, Eastern Hospital of Shanghai Sixth People’s Hospital, Shanghai 201306, China
  • Supported by:

    a grant from Shanghai Science and Technology Commission of China, No. 04419723

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被引次数

3

摘要：

背景：80%以上的腰椎间盘突出症早期患者通过非手术治疗可缓解或者治愈。针灸治疗是非手术治疗中很重要的方法之一，且疗效确切。温针是针灸疗法中很重要的一种方法，具有针刺促使气血调和、通经活络以及艾灸调和气血、舒筋通络、解瘀止痛的双重作用。
目的：观察温针对大鼠腰神经根受压模型组织形态的影响。
方法：纳入60只 SD大鼠，平均分为5组，分别为正常组、模型组、美洛昔康组、针刺组和温针组，每组12只。通过将特制的硅胶片放置在右侧L5神经根出硬膜囊处，从而对大鼠的腰神经根造成压迫，建立大鼠腰神经根受压模型。正常组不予造模，不予治疗；模型组仅造模，不予治疗；美洛昔康组造模后予以美洛昔康治疗2周；针刺组造模后予以针刺治疗2周；温针组造模后予以温针治疗2周。造模2周后取材，采用苏木精-伊红染色及光镜观察各组大鼠受压腰神经根组织形态变化。
结果与结论：动物造模后2周，经病理切片观察神经损伤情况，确认腰神经根受压模型大鼠造模成功。温针组的神经纤维及背根节周围组织炎症反应改善较美洛昔康组和针刺组明显。提示温针比美洛昔康和针刺能更有效的改善大鼠受损神经根组织结构，降低大鼠腰神经根受压模型的炎性反应。 

 

关键词: 实验动物模型, 骨及关节损伤动物模型, 温针, 针刺, 大鼠, 腰神经根受压

Abstract:

BACKGROUND: 80% of patients with early-stage lumbar disc herniation can be alleviated or cured by non-surgical treatment. Acupuncture is one of the very important non-surgical treatments, and has a definite therapeutic effect. Warm needling is an important part of acupuncture and moxibustion and exhibits the dual effects of acupuncture and moxibustion, including promoting coordination between Qi and blood, dredging the meridian passage and removing blood stasis to alleviate pain.
OBJECTIVE: To observe the effect of warm needling on histomorphology of compressed lumbar nerve root compression in rat models.
METHODS: Sixty Sprague-Dawley rats were divided into five groups: normal, model, meloxicam, acupuncture and warm needling groups (n=12 rats/group). A special silicone sheet was placed on the right side of the dural sac at the L5 nerve root to establish rat models of lumbar nerve root compression. Rats in normal group were not subjected to model establishment and treatment. Rats in model group were subjected to model establishment, but without treatment. Rats in meloxicam group were given the meloxicam treatment for 2 weeks after modeling. Rats in acupuncture group were given 2 weeks of acupuncture treatment after modeling. Rats in warm needling group were given 2 weeks of warm needling treatment after modeling. At 2 weeks after modeling, compressed lumbar nerve root tissue was harvested and stained with hematoxylin-eosin for histomorphological observation.
RESULTS AND CONCLUSION: At 2 weeks after rat models of lumbar nerve root compression were successfully established, as confirmed by pathological observation of nerve root section. Inflammatory response in the tissue surrounding the nerve fibers and dorsal root ganglia in the warm needling group better improved than in the meloxicam and acupuncture groups. These results suggest that compared with meloxicam and acupuncture, warm needling can more effectively improve the damaged rat nerve root structure and reduce the inflammatory response in rats with lumbar nerve root compression.  

 

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