中国组织工程研究

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (20): 3168-3172.doi: 10.3969/j.issn.2095-4344.3519

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

热敏灸对盆腔炎症模型大鼠影响的三焦焦膜理论分析

崔田田，易  岚，欧阳厚淦，吴慧婷，欧阳彦楚，陈  楚   

  1. 江西中医药大学中医学院，江西省南昌市  330004
  • 收稿日期:2020-06-10 修回日期:2020-06-16 接受日期:2020-07-27 出版日期:2021-07-18 发布日期:2021-01-15
  • 通讯作者: 欧阳厚淦，博士，副教授，江西中医药大学中医学院，江西省南昌市 330004
  • 作者简介:崔田田，女，1993年生，山东省临沂市人，汉族，江西中医药大学在读硕士，主要从事中西医结合基础专业研究。
  • 基金资助:
    江西省自然科学基金面上项目(20202BABL206143)，项目负责人：欧阳厚淦；江西省中医学一流学科专项科研基金项目(JXSYLXK-ZHY1028)，项目负责人：欧阳厚淦；江西省卫生计生委中医药科研计划(2018A381)，项目负责人：欧阳厚淦

Effect of thermosensitive moxibustion in a rat model of pelvic inflammation based on trifocal focal membrane theory

Cui Tiantian, Yi Lan, Ouyang Hougan, Wu Huiting, Ouyang Yanchu, Chen Chu   

  1. School of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China
  • Received:2020-06-10 Revised:2020-06-16 Accepted:2020-07-27 Online:2021-07-18 Published:2021-01-15
  • Contact: Ouyang Hougan, MD, Associate professor, School of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China
  • About author:Cui Tiantian, Master candidate, School of Traditional Chinese Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, Jiangxi Province, China
  • Supported by:
    Jiangxi Natural Science Foundation, No. 20202BABL206143 (to OYHG); Jiangxi Province First-class Chinese Medicine Special Research Fund Project, No. JXSYLXK-ZHY1028 (to OYHG); TCM Research Program of Jiangxi Health and Family Planning Commission, No. 2018A381 (to OYHG)

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摘要：

文题释义：
三焦焦膜：现代医家普遍认为其为有形实质脏器，它的实质包括胸膜、腹膜等在内的遍布人体体内胸腔、腹腔的一张网膜；所有的脏器都分居在它上、中、下三个地带，受它的包裹与保卫。
热敏灸：全称“腧穴热敏化艾灸新疗法”又称“热敏悬灸”，可激发透热、扩热、传热、局部不热远部热、非热觉等热敏灸感和经气传导，其具有不接触人体、无伤害、无不良反应、绿色新型等特点。

背景：从临床经验和基础实验得出热敏灸对于盆腔炎的治疗有着积极作用，但是它的作用机制还未解释完全。
目的：从焦膜角度探讨热敏灸对盆腔炎大鼠模型筋膜结缔组织的影响及其治疗机制。
方法：SPF级雌性大鼠60只，用随机数表法将大鼠分为正常组(n=15)和造模组(n=45)。采用混合菌注入法制备盆腔炎模型，造模后根据尾温变化将45只造模组随机分为模型组、艾灸组、热敏灸组。正常组和模型组不使用任何干预措施，剩余两组皆选取“三阴交”“足三里”及“关元”作为治疗穴位，艾灸组艾灸15 min/d，热敏灸组艾灸40 min/d；治疗都以7 d作为观察周期。疗程周期结束后采用MASSON染色法观测穴位局部筋膜、腹膜以及子宫形态学变化，用ELISA法测定各组大鼠血清中白细胞介素6、肿瘤坏死因子α水平。
结果与结论：①穴位局部筋膜、腹膜以及子宫形态学变化：与模型组相比，热敏灸组大鼠各处筋膜MASSON染色中胶原纤维呈波浪式分布，胞浆直径变长；②血清指标：模型组大鼠血清中白细胞介素6、肿瘤坏死因子α水平较正常组显著增高(P < 0.05)，经治疗过后，热敏灸组、艾灸组血清中2种炎症因子水平比模型组明显下降(P < 0.01)，而热敏灸组又比艾灸组下降明显(P < 0.05)；③结果提示：热敏态穴位发挥作用可能与全身的三焦焦膜密切相关，热敏灸可能通过改变全身有关结缔组织成纤维细胞形态结构实现热信号传导；热敏灸缓解盆腔炎性症状可能与通过改变血清中白细胞介素6、肿瘤坏死因子α水平有关。

关键词: 热敏灸, 三焦焦膜, 盆腔炎, 大鼠, 艾灸, 筋膜, 实验

Abstract: BACKGROUND: From clinical experience and basic experiments, it is concluded that thermosensitive moxibustion has a positive effect on the treatment of pelvic inflammatory disease, but its mechanism of action has not been fully explained.
OBJECTIVE: To investigate the effect of thermosensitive moxibustion on fascial connective tissue and its therapeutic mechanism in rats with pelvic inflammatory disease.
METHODS: Sixty SPF female rats were randomly divided into normal group (n=15) and model group (n=45). The pelvic inflammatory disease model was prepared by mixed bacteria injection. After modeling, 45 model rats were randomly divided into model group, moxibustion group and thermosensitive moxibustion group according to the change of tail temperature. The normal and model groups did not receive any intervention measures, and the remaining two groups were given moxa-moxibustion (15 minutes per day) and thermosensitive moxibustion (40 minutes per day) at Sanyin Jiao, Zusanli and Guanyuan acupoints, respectively. All treatments were observed over a 7-day period. At the end of the treatment, MASSON staining was used to observe the morphological changes of the uterus, peritoneum and fascia around the acupoints. The contents of interleukin-6 and tumor necrosis factor-α in the rat serum were determined by ELISA. 
RESULTS AND CONCLUSION: MASSON staining of the uterus, peritoneum and fascia around the acupoints showed that, compared with the model group, the collagen fibers in the fascia of the thermosensitive moxibustion group showed wavy distribution and the diameter of cytoplasm became longer. The serum levels of tumor necrosis factor-α and interleukin-6 in the model group were significantly higher than those in the normal group (P < 0.05). After treatment, the levels of tumor necrosis factor-α and interleukin-6 in the thermosensitive moxibustion group and the moxibustion group were significantly lower than those in the model group (P < 0.01), while the levels of tumor necrosis factor-α and interleukin-6 in the thermosensitive moxibustion group were significantly lower than those in the moxibustion group (P < 0.05). The results suggest that the role of thermosensitive acupuncture points may be closely related to the trifocal film of the whole body, and thermosensitive moxibustion may realize the heat signal conduction by changing the morphology and structure of fibroblasts in the relevant connective tissue of the whole body. The relief of pelvic inflammatory symptoms by thermosensitive moxibustion may be related to the changes in serum levels of tumor necrosis factor-α and interleukin-6.

Key words:  , thermosensitive moxibustion, trifocal film, pelvic inflammation, rat, moxa-moxibustion, fascia, experiment

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