中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (18): 2879-2883.doi: 10.3969/j.issn.2095-4344.2015.18.016

• 生物材料模型构建 biomaterial modeling • 上一篇    下一篇

构建截肢创伤模型大鼠肝脏功能变化及硫化氢的影响

张  颖，张海峰，任青爱，谢晓华   

  1. 解放军总医院南楼综合外科，北京市  100853
  • 收稿日期:2015-04-07 出版日期:2015-04-30 发布日期:2015-04-30
  • 通讯作者: 谢晓华，硕士，主任医师，教授，博士生导师，解放军总医院南楼综合外科，北京市 100853
  • 作者简介:张颖，男，1977年生，重庆市人，汉族，解放军医学院在读博士，主治医师，主要从事围手术期器官损伤与防护研究。
  • 基金资助:

    军队“十一五”科技攻关课题(06G117)；军队十二五重点科研课题(BWS12J051)

Effects of hydrogen sulfide on liver function in rat models of amputation

Zhang Ying, Zhang Hai-feng, Ren Qing-ai, Xie Xiao-hua   

  1. Department of General Surgery, South Building, Chinese PLA General Hospital, Beijing 100853, China
  • Received:2015-04-07 Online:2015-04-30 Published:2015-04-30
  • Contact: Xie Xiao-hua, Master, Chief physician, Professor, Doctoral supervisor, Department of General Surgery, South Building, Chinese PLA General Hospital, Beijing 100853, China
  • About author:Zhang Ying, Studying for doctorate, Attending physician, Department of General Surgery, South Building, Chinese PLA General Hospital, Beijing 100853, China
  • Supported by:

    the Army “Eleventh Five-Year” Science and Technology Project, No. 06G117; the Army “Twelfth Five-Year” Key Science and Technology Program, No. BWS12J051

PDF

359

摘要：

背景：截肢是一种特殊类型的创伤，创伤导致肝脏损害的机制及硫化氢对肝脏的作用目前尚不明确。
目的：探索构建截肢创伤模型大鼠的肝脏损伤，以硫化氢(H2S)对其的影响。
方法：将Wistar大鼠和SD大鼠分别随机等分为正常组、截肢后6，12，24，72 h组、硫氢化钠(NaHS)组、炔丙基甘氨酸组和为正常组、截肢后6 h组、NaHS组、炔丙基甘氨酸组。除正常组外所有大鼠完全截断左侧于膝关节上方1.2-1.4 cm处结构，并在结扎血管后剪断左侧股静、动脉，建立左后肢的截肢模型大鼠。NaHS组及炔丙基甘氨酸组大鼠分别于截肢后即刻腹腔注射28 μmol/kg NaHS和50 mg/kg炔丙基甘氨酸。
结果与结论：与正常组相比，截肢后6 h组大鼠肝脏和线粒体结构出现了损伤性改变，血浆及肝脏髓过氧化物酶、丙二醛、H2S/胱硫醚γ-裂解酶水平、血浆及肝脏髓过氧化物酶、丙二醛、肝脏线粒体呼吸控制率、膜电位及ATP酶活性明显降低(P < 0.05)，NaHS干预后H2S/胱硫醚γ-裂解酶水平及以上指标明显升高(P < 0.05)，但血浆转氨酶并未明显变化(P > 0.05)。应用炔丙基甘氨酸后，以上除线粒体指标无明显变化外，多表现为进一步降低，转氨酶也明显下降(P < 0.05)。说明H2S可减轻截肢模型大鼠肝脏组织脂质过氧化、炎症反应，并使线粒体功能明显改善，但并未减轻肝脏功能的受损。



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

关键词: 实验动物, 消化系统损伤模型, 硫化氢, 肝损害, 创伤, 线粒体, 截肢, 脂质过氧化, 炎症反应

Abstract:

BACKGROUND: Amputation is a special type of trauma. Mechanism of trauma-induced damage to the liver and the effects of hydrogen sulfide (H2S) on the liver remain unclear.
OBJECTIVE: To explore the mechanism of hepatic damage in rats of postoperative amputation, and how H2S exerts effects on liver function.
METHODS: Wistar rats and Sprague-Dawley rats were equally and randomly divided into normal group, postoperative 6-, 12-, 24- and 72-hour groups, sodium hydrosulfide (NaHS) group, and propargyl glycine group, as well as normal group, postoperative 6-hour group, HaHS group, and propargyl glycine group. Except the normal group, the structure 1.2-1.4 cm above the left knee was completely transected in rats of other groups. Blood vessels were ligated, and then left femoral vein and femoral artery were cut to establish rat models of amputation in the left hind limb. In the NaHS and propargyl glycine groups, 28 µmol/kg NaHS and 50 mg/kg propargyl glycine were intraperitoneally injected immediately after amputation.
RESULTS AND CONCLUSION: Compared with the normal group, traumatic changes in rat liver cells and mitochondrial structure were seen, and plasma and liver myeloperoxidase, malondialdehyde, H2S/cystathionine γ-lyase levels, liver mitochondrial respiratory control rate, membrane potential and ATP activity were significantly lower in the postoperative 6-hour groups (P < 0.05). After NaHS intervention, H2S/cystathionine γ-lyase level and above indicators were significantly higher in the postoperative 6-hour groups (P < 0.05), but plasma transaminase did not significantly alter (P > 0.05). After treatment with propargyl glycine, above indicators except mitochondrial indexes were further decreased, and transaminase was significantly reduced (P < 0.05). These findings suggest 
that H2S can reduce lipid peroxidation, inflammatory reaction, and make the mitochondrial function improved significantly, but did not reduce the damage of liver function in rat models after amputation.



中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程


全文链接：

Key words: Hydrogen Sulfide, Amputation, Liver, Mitochondria

中图分类号: