关键词: 生物材料, 材料相容性, 羟基磷灰石, 人脐带静脉血管内皮细胞, 细胞毒性, 活细胞计数试剂盒, 安徽省自然科学基金

Abstract:

BACKGROUND: Pulsed laser deposition synthesis technology has been used to prepare new nano-hydroxyapatite thin film coating by collagen deposition on artificial mechanical heart valve. 
OBJECTIVE: To investigate the toxicity of new nano-hydroxyapatite thin film on human umbilical vein endothelial cells.
METHODS: Human umbilical vein endothelial cells were cultured with nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, high-density polyethylene and phenol solution. Within 72 hours, cell growth was observed under the inverted phase contrast microscope. At 7 days after culture, cell proliferation and toxicity grading were detected using Cell Counting Kit-8. 
RESULTS AND CONCLUSION: At 24 hours after culture, cells grew well, showed fusiform shape, and had strong refraction in the nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, high-density polyethylene groups, and no significant differences in cell morphology and number were detected among above groups. Cells in the phenol solution group were suspended, round, pyknotic and dead. At 48 hours, except phenol solution group, cell number increased significantly, and cell grew densely in other three groups. At 72 hours, cells grew strongly, and the gap became small obviously. Within 7 days after culture, cell proliferation activity was not significant in the nano-hydroxyapatite film room-temperature leaching solution, nano-hydroxyapatite film high-temperature leaching solution, and high-density polyethylene groups, which was significantly higher than in the phenol solution group (P < 0.05). The toxicity of nano-hydroxyapatite film graded 0 to 1. These results suggested that nano-hydroxyapatite artificial mechanical heart valve has good histocompatibility, but no toxicity.

Key words: Durapatite, Endothelial Cells, Umbilical Veins