中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (39): 6329-6334.doi: 10.3969/j.issn.2095-4344.2014.39.017

• 细胞外基质材料 extracellular matrix materials • 上一篇    下一篇

同种异体富血小板血浆可增强糖尿病大鼠合成创面胶原

刘  宸1，章宏伟1，徐  宁2   

  1. 1南京医科大学附属第一临床医学院，江苏省人民医院整形科，江苏省南京市  210000；2隆德大学临床检验医学系化学药理部，瑞典隆德，S-221 85
  • 出版日期:2014-09-17 发布日期:2014-09-17
  • 通讯作者: 章宏伟，主任医师，南京医科大学附属第一临床医学院，江苏省人民医院整形科，江苏省南京市 210000
  • 作者简介:刘宸，女，1988年生，江苏省镇江市人，汉族，2014年南京医科大学毕业，硕士，医师，主要从事组织工程及组织移植等方向研究。
  • 基金资助:

    江苏省“六大人才高峰”资助项目(2011IB12)

Allogeneic platelet-rich plasma promotes wound collagen synthesis in diabetic rats

Liu Chen1, Zhang Hong-wei1, Xu Ning2   

  1. 1 Department of Burns and Reconstructive Surgery, Jiangsu Provincial People’s Hospital, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, Jiangsu Province, China; 2 Division of Clinical Chemistry and Pharmacology, Department of Laboratory Medicine, Lund University, Lund S-221 85, Sweden
  • Online:2014-09-17 Published:2014-09-17
  • Contact: Zhang Hong-wei, Chief physician, Department of Burns and Reconstructive Surgery, Jiangsu Provincial People’s Hospital, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, Jiangsu Province, China
  • About author:Liu Chen, Master, Physician, Department of Burns and Reconstructive Surgery, Jiangsu Provincial People’s Hospital, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, Jiangsu Province, China
  • Supported by:

    Six Talents Peak Project of Jiangsu Province, No. 2011IB12

PDF

544

被引次数

13

摘要：

背景：研究显示，自体富血小板血浆有助于促进糖尿病创面的愈合，但同种异体来源的富血小板血浆对糖尿病创面的作用尚不明确。
目的：以链脲佐菌素诱导的糖尿病大鼠创面模型为观察对象，观察同种异体来源的富血小板血浆对糖尿病创面胶原合成的作用。
方法：30只SD大鼠，采用链脲佐菌素诱导形成糖尿病模型鼠，随机分成富血小板血浆组和糖尿病组，每组15只。于大鼠背部，形成面积为1 cm2的全层皮肤缺损创面，分别涂以0.1 mL同种异体富血小板血浆或生理盐水，3M贴膜封闭，胶布固定。分别于造模后3，7，14 d时每组各取5只动物观察创面愈合率，并切取创面组织行Masson染色观察胶原分布形态、检测创面组织中羟脯氨酸含量及Ⅰ型、Ⅲ型胶原的mRNA相对表达量。
结果与结论：造模后第3，7，14天时富血小板血浆组的创面愈合率均高于糖尿病组(P < 0.05)。Masson 染色结果显示富血小板血浆组的创面胶原形成较快、且纤维更粗壮、排列更致密。羟脯氨酸检测结果显示，富血小板血浆组的创面中羟脯氨酸含量明显高于糖尿病组(P < 0.01)。RT-PCR检测结果显示术后第3、7、14天时，富血小板血浆组创面内Ⅰ型和Ⅲ型胶原的mRNA表达量均高于糖尿病组(P < 0.05)。同时Ⅰ/Ⅲ型胶原比值也较糖尿病组明显增高(P < 0.05)。结果表明同种异体来源的富血小板血浆可以有效促进糖尿病创面的愈合，这可能与其增强创面胶原合成的作用有关。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 材料相容性, 富血小板血浆, 胶原合成, 糖尿病创面, 创面愈合

Abstract:

BACKGROUND: Platelet-rich plasma has benefit in diabetic wound healing; however, the effect of allogeneic platelet-rich plasma is still unclear.
OBJECTIVE: To observe the effect of allogeneic platelet-rich plasma on collagen synthesis during diabetic wound healing.
METHODS: Streptozocin-induced diabetic rats were randomly divided into allogeneic platelet-rich plasma group and saline control group. Each group had 15 rats. A 1 cm2 full-thickness skin defect on the rat dorsum was excised. Platelet-rich plasma or saline was applied. Platelet-rich plasma was prepared from the whole blood of allogeneic healthy rats. Animals of each group were sacrificed on 3, 7, 14 days post operation. The differences of the wound closure rate, morphological character, hydroxyproline content and the relative mRNA expression of the collagen were observed.
RESULTS AND CONCLUSION: The wound closure rates were lower in control group on day 3, 7, 14 post operation (P < 0.05). Masson staining showed a decreased, disorder, loose collagen fibers distribution in diabetic control wound tissue. The results of the hydroxyproline test showed hydroxyproline content was significantly higher in platelet-rich plasma group at each time point (P < 0.01). The relative mRNA expression of type I and III  collagen presented a higher expression in platelet-rich plasma treated wound tissue at each time point (P < 0.05). And the ratio between type I and III collagen was higher in platelet-rich plasma group at each time point (P < 0.05). In summary, allogeneic platelet-rich plasma can promote diabetic wounds healing, which may attribute to an enhanced collagen synthesis.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: platelet-rich plasma, collagen, wound healing, diabetes mellitus

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