中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (39): 6240-6245.doi: 10.3969/j.issn.2095-4344.2014.39.002

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

纯钛表面阳极氧化增强成骨细胞生物活性及护骨素基因的表达

付晓龙1，李  莺1，李宝娥2，李长义1   

  1. 1天津医科大学口腔医院，天津市  300070；2河北工业大学材料科学与工程学院，天津市  300019
  • 出版日期:2014-09-17 发布日期:2014-09-17
  • 通讯作者: 李长义，博士，教授，主任医师，天津医科大学口腔医院，天津市 300070
  • 作者简介:付晓龙，男，1988 年生，山东省青岛市人，汉族，天津医科大学口腔医院在读硕士，主要从事口腔种植材料方面的研究。
  • 基金资助:

    天津市高等学校科技发展基金项目(20120126)，课题名称“种植体表面仿生图案促进成骨细胞活性的分子网络研究”；河北省自然科学基金(E2013202021)，课题名称“阳极氧化法改性钛合金表面生物学性能的研究”；中国科学院特种无机涂层重点实验室2012年度开放基金(KLICM-2012-02)，课题名称“钛合金表面纳米化和功能化研究”

Improvement of osteoblast bioactivity and osteoprotegrin gene expression of titanium surface by anodic oxidation

Fu Xiao-long1, Li Ying1, Li Bao-e2, Li Chang-yi1   

  1. 1 Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China; 2 School of Materials Science and Engineering, Hebei University of Technology, Tianjin 300130, China
  • Online:2014-09-17 Published:2014-09-17
  • Contact: Li Chang-yi, M.D., Professor, Chief physician, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
  • About author:Fu Xiao-long, Studying for master’s degree, Stomatological Hospital of Tianjin Medical University, Tianjin 300070, China
  • Supported by:

    the Scientific and Technological Developmental Foundation in Tianjin Universities, No. 20120126; the Natural Science Foundation of Hebei Province, No. E2013202021; Foundation of Key Laboratory of Inorganic Coating Materials, Chinese Academy of Sciences, No. KLICM-2012-02

PDF

583

被引次数

8

摘要：

背景：纯钛阳极氧化改性后形成的纳米结构与骨组织具有良好的生物相容性。
目的：观察纯钛表面纳米孔结构的形貌和物相构成，以及其对MC3T3-E1小鼠前成骨细胞增殖、黏附等生物学行为和促成骨基因护骨素表达的影响。
方法：取纯钛片24份，其中12份仅进行机械抛光，作为对照组；另外12份进行机械抛光后，应用阳极氧化技术在纯钛表面制备纳米孔结构，作为实验组。将小鼠前成骨细胞MC3T3-E1分别接种于两组试件表面，接种7 d后扫描电镜下观察细胞形态，采用MTT法检测细胞增殖情况，绘制生长曲线；同时检测细胞促成骨基因护骨素的表达。
结果与结论：阳极氧化后钛片表面形成规格统一的纳米孔结构，但是物相构成并未发生变化。与接种于对照组试件上的成骨细胞相比，实验组试件表面的细胞密度变大，覆盖金属的面积更多，呈现多边形结构，突触向周围移行，可见板状伪足向周围材料伸出；接种第7天时，实验组细胞数目约为对照组的1.4倍，同时纳米孔表面成骨细胞护骨素基因的表达高于对照组(P < 0.01)。结果表明阳极氧化后形成纳米孔结构的钛片更有利于成骨细胞的黏附、增殖和护骨素基因的表达，进而促进成骨细胞生长，具有良好的生物相容性。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 口腔生物材料, 阳极氧化, 钛, 纳米孔, 成骨细胞, 增殖, 生物活性, 护骨素, 河北省自然科学基金

Abstract:

BACKGROUND: Nanostructure formation on titanium surface by anodic oxidation has good biocompatibility with bone tissue.
OBJECTIVE: To observe the surface morphology and crystalline constitution of nanopores microstructure on titanium surface formed by anodic oxidation and to further observe its influence on the MC3T3-E1 osteoblast cells’ biological behavior and the gene expression of osteoprotegerin.
METHODS: Nanopores forming on titanium surface by anodic oxidation was prepared as experimental group and polished titanium as control group (12 samples for each group). Mouse MC3T3-E1 osteoblasts were co-cultured with polished pure titanium plate group and anodic oxidation nanopores group. After 7 days of inoculation, cell morphology was observed using scanning electron microscopy, MTT method was used for the cell proliferation test and the growth curve was made. Gene expression of osteoprotegerin was also detected.
RESULTS AND CONCLUSION: After anodic oxidation, a homogeneous and uniform array of nanopores formed;  however it had no significant influence on the crystalline phase of the titanium sample surfaces. Titanium surface with nanopore structure was more favorable than polished titanium surface for cell attachment and spreading. Cells on the anodized surface with nanopores had higher cell density and bigger metal coverage area. Cells on the nanopores surface also exhibited a polygonal shape with many filopodia extending in all directions. MTT method showed that the anodized nanopore surface had higher cell amount than the as-polished titanium, and the former was about 1.4 times of the latter group after 7 days of culture. The gene expression level of osteoprotegerin in the MC3T3-E1 cells cultured on anodized titanium surface with nanopores was significantly higher than that on the as-polished titanium (P < 0.01). The anodic oxidation treatment is more advantageous for the osteoblasts adhesion and gene expression of osteoprotegerin, thereby promoting the growth of osteoblasts.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: titanium, nanopores, osteoblasts

中图分类号: