中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (8): 1205-1210.doi: 10.3969/j.issn.2095-4344.2014.08.010

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

反相高效液相色谱法测定壳聚糖纳米粒中丙酮酸乙酯的含量

徐凤娥，赵兵新，孙树茂，孟永春，南开辉，俞阿勇，瞿  佳   

  1. 温州医科大学附属眼视光医院，浙江省温州市  325000
  • 收稿日期:2013-12-02 出版日期:2014-02-19 发布日期:2014-02-19
  • 通讯作者: 瞿佳，美国新英格兰视光学院荣誉博士，教授，温州医科大学校长，温州医科大学附属眼视光医院，浙江省温州市 325000 通讯作者：俞阿勇，博士，副教授，温州医科大学附属眼视光医院，浙江省温州市 325000
  • 作者简介:徐凤娥，女，1986年生，江西省上饶市人，汉族，温州医科大学在读硕士，主要从事眼科生物材料与临床研究。
  • 基金资助:

    浙江省重点科技创新团队计划资助项目(2009R50039，2011R09039-06)

Determination of ethyl pyruvate in chitosan nanoparticles by reversed-phase high-performance liquid chromatography

Xu Feng-e, Zhao Bing-xin, Sun Shu-mao, Meng Yong-chun, Nan Kai-hui, Yu A-yong, Qu Jia   

  1. The Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
  • Received:2013-12-02 Online:2014-02-19 Published:2014-02-19
  • Contact: Qu Jia, Professor, the Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China Yu A-yong, M.D., Associate professor, the Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
  • About author:Xu Feng-e, Studying for master’s degree, the Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
  • Supported by:

    the Major Scientific Innovative Team Plan of Zhejiang Province, No. 2009R50039, 2011R09039-06

PDF

933

被引次数

3

摘要：

背景：有关丙酮酸乙酯含量检测方法的研究较少，采用反相高效液相色谱法检测丙酮酸乙酯含量的文献更少。
目的：建立测定壳聚糖纳米粒中丙酮酸乙酯含量的反相高效液相色谱法。
方法：采用Agilent1200系列高效液相色谱仪检测丙酮酸乙酯-壳聚糖纳米粒中丙酮酸乙酯的含量。色谱柱：ZORBAX Eclipse XDB-C18(4.6 mm×150 mm，5 μm)，柱温25 ℃，流动相为乙腈-水(体积比为40∶60)，流速1 mL/min，检测波长210 nm，进样量20 μL。
结果与结论：丙酮酸乙酯峰与辅料及溶剂峰分离良好，丙酮酸乙酯质量浓度在1-100 mg/L内于峰面积线性关系良好(r =0.999 6)，低、中、高浓度丙酮酸乙酯对照品溶液日内、日间精密度的相对标准偏差均小于3%，重复性实验的相对标准偏差为1.25%，稳定性实验的相对标准偏差为1.3%。3批样品的加样回收率分别为(91.5±1.0)%，(93.5±0.2)%，(94.4±0.4)%；包封率分别为(87.20±0.22)%，(90.50±0.15)%，(91.10±0.17)%。不同批次样品丙酮酸乙酯含量检测结果的相对标准偏差分别为0.9%，0.5%，0.3%。说明反相高效液相色谱法灵敏度高、线性范围宽、专属性强、精密度高、加样回收率好、结果精确可靠，可用于壳聚糖纳米粒中丙酮酸乙酯含量的测定。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 纳米材料, 药物控释材料, 壳聚糖纳米粒, 丙酮酸乙酯, 反相高效液相色谱, 包封率, 相对标准偏差

Abstract:

BACKGROUND: Research on ethyl pyruvate detection methods is reported rarely, and moreover, literature about reversed-phase high-performance liquid chromatography (RP-HPLC) for detection of ethyl pyruvate is less.
OBJECTIVE: To establish an RP-HPLC method for determination of ethyl pyruvate in ethyl pyruvate-chitosan nanoparticles.
METHODS: The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column (4.6 mm×
150 mm, 5 μm) at 25 ℃, with the mixture of acetonitrile and water (40:60, V/V) as the mobile phase at the flow rate of 1 mL/min. The determination wavelength was set at 210 nm and the injection volume was 20 μL.
RESULTS AND CONCLUSION: The peak of ethyl pyruvate and the peaks of auxiliary materials and solvent were separated well. The linear rang of ethyl pyruvate was 1-100 mg/L (r=0.999 6). The relative standard deviation of both the intra-and inter-day precision was less than 3% for low-, moderate-, and high-concentration ethyl pyruvate. The relative standard deviation of reproducibility test and stability test was 1.25% and 1.3%, respectively. Sample average recovery rates were (91.5±1.0)%, (3.5±0.2)%, (94.4±0.4)%, respectively. Encapsulation efficiency of samples were (87.2±0.22)%, (90.5±0.15)%, (91.1±0.17)%, respectively. The relative standard deviation of different sample content were 0.9%, 0.5%, 0.3%, respectively. The RP-HPLC method for determination of ethyl pyruvate is sensitive, accurate and highly specific with wide linear range and high sample average recovery.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: biocompatible materials, chitosan, nanoparticles, pyruvic acid

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