中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (32): 5834-5839.doi: 10.3969/j.issn.2095-4344.2013.32.014

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

人羊膜上皮细胞移植急性肝损伤小鼠的定量效果分析

罗宏武，寻  权，黄湘俊，黄飞舟   

  1. 中南大学湘雅三医院普外一科，湖南省长沙市  413000
  • 收稿日期:2012-11-07 修回日期:2012-11-20 出版日期:2013-08-06 发布日期:2013-08-06
  • 通讯作者: 寻权，硕士，中南大学湘雅三医院普外一科，湖南省长沙市 413000 xun155067@163.com
  • 作者简介:罗宏武☆，男，1970年，湖南省宁乡县人，汉族，2008年中南大学毕业，博士，教授，硕士生导师，主要从事肝胆外科的研究。

Quantitative analysis of transplanted effect of human amniotic epithelial cells in mice with acute liver injury

Luo Hong-wu, Xun Quan, Huang Xiang-jun, Huang Fei-zhou   

  1. First Department of General Surgery, Xiangya Third Hospital, Central South University, Changsha  413000, Hunan Province, China
  • Received:2012-11-07 Revised:2012-11-20 Online:2013-08-06 Published:2013-08-06
  • Contact: Xun Quan, Master, First Department of General Surgery, Xiangya Third Hospital, Central South University, Changsha 413000, Hunan Province, China xun155067@163.com
  • About author:Luo Hong-wu☆, M.D., Professor, Master’s supervisor, First Department of General Surgery, Xiangya Third Hospital, Central South University, Changsha 413000, Hunan Province, China

PDF

317

被引次数

1

摘要：

背景：目前已有较多关于人羊膜上皮细胞移植入动物体内的存活、迁徙及相关特性的初步研究，但其对移植效果的定量分析尚未见报道。
目的：对脾内移植传代的人羊膜上皮细胞小鼠血清肝生化功能及人血白蛋白的定量分析。
方法：40只裸小鼠随机分为4组，每组各10只。肝叶切除+细胞移植2周组、肝叶切除+细胞移植4周组、肝叶切除+盐水组，行半肝叶切除，肝叶切除+细胞移植组自脾下极移植密度为5×106传代的人羊膜上皮细胞约0.2 mL，分别于移植后2周和4周采血；肝叶切除+盐水组自脾下极注射生理盐水0.2 mL；单纯细胞移植组：不行肝叶切除，自脾下极移植密度为5×106传代的人羊膜上皮细胞约0.2 mL。检测其各组肝脾组织学、形态学的改变及各组血清谷丙转氨酶、谷草转氨酶、人血白蛋白的变化和人血白蛋白表达定量分析。
结果与结论：人羊膜上皮细胞移植急性肝损伤小鼠4周后肝脾形态未见明显改变，组织学可检测到特异性细胞，血清谷丙转氨酶、谷草转氨酶、人血白蛋白有明显改善，血清中能检测到人血白蛋白且移植后4周较移植后2周有明显升高。因此，人羊膜上皮细胞移植入肝受损小鼠体内能存活超过4周且仍表达肝细胞样细胞的部分特性及功能，改善小鼠的肝功能，治疗小鼠急性肝损伤。

关键词: 干细胞, 干细胞移植, 人羊膜上皮细胞, 脾内移植, 急性肝损伤, 肝功能, 人血白蛋白, 谷丙转氨酶, 谷草转氨酶, 定量分析, 肝脏, 脾脏, 干细胞图片文章

Abstract:

BACKGROUND: There are many preliminary studies on the survival, metaptosis, and correlation characteristics of human amniotic epithelial cells after transplanted into the animals, but there are no reports on the quantitative analysis of the transplantation effect.
OBJECTIVE: To make quantitative analysis on serum biochemical function of liver and the expression of human albumin in mice received passaged human amniotic epithelial cells transplantation in spleen.
METHODS: Forty nude mice were randomly divided into four groups (n=10 in each group): hepatectomy+cell transplantation 2 weeks group, hepatectomy+cell transplantation 4 weeks group, hepatectomy+normal saline group (treated with partial hepatectomy) and hepatectomy+cell transplantation group (transplanted with 0.2 mL passaged human amniotic epithelial cells with 5×106 under spleen, and the blood were collected at 2 and 4 weeks after transplantation). The mice in the hepatectomy+normal saline group were treated with splenic injection of  0.2 mL normal saline; the cell transplantation group did not receive hepatectomy, and transplanted with 0.2 mL passaged human amniotic epithelial cells with 5×106 under spleen. The histological and morphological changes of the liver and spleen in each group as well as the expressions of serum alanine aminotransferase, aspartate aminotransferase and human serum albumin in each group were detected, and the quantitative analysis of human serum albumin expression was performed.
RESULTS AND CONCLUSION: There was no obvious morphological change after human amniotic epithelial cells transplanted into the acute liver injury mice for 4 weeks, but specific cells could be detected by histological method. The serum levels of alanine aminotransferase, aspartate aminotransferase and human serum albumin were improved obviously, and the human albumin could be detected in serum, the level of human albumin at 4 weeks after transplantation was significantly increased than 2 weeks after transplantation. Human amniotic epithelial cells can survive for more than 4 weeks after transplanted into the liver injury mice, and can still express partial characteristics and functions of hepatocyte-like cells, improve the liver function, thus treating acute liver injury.

Key words: stem cells, stem cell transplantation, human amniotic epithelial cells, intrasplenic transplantation, acute liver injury, liver function, human albumin, alanine aminotransferase, aspartate aminotransferase, quantitative analysis, liver, spleen, stem cell photographs-containing paper

中图分类号: