中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (52): 9792-9796.doi: 10.3969/j.issn.2095-4344.2012.52.020

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

几丁糖干预兔髋关节囊切开-愈合模型基质金属蛋白酶2的表达

郭俊斌，李昭铸   

  1. 哈尔滨医科大学附属第二医院小儿外科，黑龙江省哈尔滨市 150086
  • 收稿日期:2012-04-09 修回日期:2012-06-24 出版日期:2012-12-23 发布日期:2012-12-23
  • 作者简介:郭俊斌☆，男，1975年生，山西省武乡县人，汉族，2005年中国医科大学毕业，博士，副主任医师，主要从事小儿外科疾病研究。 guojunbin@yahoo.com.cn

Expression of matrix metalloproteinase 2 in rabbit models of hip joint capsule cut-healing after chitosan intervention

Guo Jun-bin, Li Zhao-zhu   

  1. Department of Pediatric Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Received:2012-04-09 Revised:2012-06-24 Online:2012-12-23 Published:2012-12-23
  • About author:Guo Jun-bin☆, Doctor, Associate chief physician, Department of Pediatric Surgery, the Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China guojunbin@yahoo.com.cn

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474

被引次数

2

摘要：

背景：发育性髋关节脱位是小儿骨科常见畸形之一，预防手术后关节粘连主要手段是功能练习，但锻炼的时机选择则受限于关节囊愈合情况。
目的：观察兔关节囊切开后愈合过程的形态学以及几丁糖干预后基质金属蛋白酶2的表达，探讨关节囊愈合机制。
方法：新西兰大白兔制作关节囊切开-愈合模型，左侧(实验侧)在关闭前注入医用几丁糖1 mL，右侧(对照侧)则仅行切开后关闭，不予几丁糖干预。各组在处死兔的第一时间取髋关节囊，所得标本采用Masson 染色法行胶原染色分析和ABC免疫组织化学法检测基质金属蛋白酶2表达。
结果与结论：胶原染色显示术后3-28 d，对照侧和实验侧的胶原分布和排列均呈逐渐变好趋势，但早期水肿和断裂明显。对照侧的胶原纤维形态完整性、断裂间隙和排列致密与规律程度均差于实验侧。基质金属蛋白酶2表达检测结果则显示对照侧和实验侧基质金属蛋白酶2表达均为阳性，其强度规律表现为随时间增加呈逐渐加强趋势，但是并不完全同步，其中对照侧在术后7 d有明显下降随后则开始增强，但最终仍弱于实验侧，实验侧则呈增强趋势，除术后3 d弱于对照侧外，其余各时间点表达明显强于对照侧。结果可见关节囊愈合过程中基质金属蛋白酶2通过降解变性胶原明胶的特异能力参与重建，而且重建塑形过程要大于28 d，几丁糖可以延缓早期胶原组织变性、抑制炎性反应并促进愈合。

关键词: 发育性髋关节脱位, 愈合, 胶原纤维, 基质金属蛋白酶2, 几丁糖, 医学植入体

Abstract:

BACKGROUND: The developmental dislocation of the hip is a kind of common orthopaedics deformity. Function practice is the main measure to prevent articular adhesion after operation. But the right time choice depends on the healing degree of the capsule of hip joint.
OBJECTIVE: To observe the morphology of hip joint capsule cut-healing model of the rabbits and the expression of matrix metalloproteinase 2 after chitosan induction, so as to explore the healing mechanism of hip capsule.
METHODS: New Zealand white rabbit was used to establish the hip joint capsule cut-healing model, the left hip joint capsule (experimental group) was injected with 1 mL chitosan before closure, and the right hip joint capsule (control group) was closed after cutting without chitosan induction. The capsules were immediately obtained after executed the rabbit, then the collagen analysis was performed by Masson staining, and the expression of matrix metalloproteinase 2 was detected by ABC immunohistochemical method.
RESULTS AND CONCLUSION: The collagen staining showed that collagen distribution and arrangement in these two groups were gradually changed to the better trend from 3 days to 28 days, but the early edema and fracture were obvious. The integrity of the collagen fiber morphology, the fracture gap and arrangement dense and regularity level in the control group were worse than those in the experimental group. Detection of the matrix metalloproteinase 2 showed that the expression of matrix metalloproteinase 2 was positive in both groups and grown stronger with the time increasing, but not fully synchronized. The expression of matrix metalloproteinase 2 in the control group was decreased at 7 days after operation and finally weaker than that in the experimental group, while the expression in the experimental group was continued increasing and stronger than that in the control group, excepted for the 3rd day after operation. Matrix metalloproteinase 2 participates in the reconstruction process through digesting degenerated collagen, and the reconstruction process should be longer than 28 days. Chitosan can promote healing process through delaying tissue necrosis degeneration and depressing inflammatory reaction.

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