中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8067-8070.doi: 10.3969/j.issn.2095-4344.2012.43.019

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

中空钛合金假体壁上孔洞直径对细胞生长的影响

樊宇平1，黄靖香2，蔡 胥3   

  1. 1内蒙古乌兰察布市中心医院骨科，内蒙古自治区乌兰察布市 012000；解放军总医院，2骨科实验室；3骨科，北京市 100853
  • 收稿日期:2012-02-02 修回日期:2012-03-02 出版日期:2012-10-21 发布日期:2012-10-21
  • 通讯作者: 蔡胥，博士，主任医师，解放军总医院骨科，北京市 100853
  • 作者简介:樊宇平★，男，1970年生，内蒙古自治区凉城县人，汉族，2007年解放军总医院军医进修学院毕业，硕士，副主任医师，主要从事人工关节的研发。 Fanyuping1024@126.com

Effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells

Fan Yu-ping1, Huang Jing-xiang2, Cai Xu3   

  1. 1Department of Orthopedics, Ulanqab Central Hospital, Ulanqab 012000, Inner Mongolia Autonomous Region, China; 2Orthopedics Laboratory, 3Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • Received:2012-02-02 Revised:2012-03-02 Online:2012-10-21 Published:2012-10-21
  • Contact: Cai Xu, Doctor, Chief physician, Department of Orthopedics, General Hospital of Chinese PLA, Beijing 100853, China
  • About author:Fan Yu-ping★, Master, Associate chief physician, Department of Orthopedics, Ulanqab Central Hospital, Ulanqab 012000, Inner Mongolia Autonomous Region, China Fanyuping1024@ 126.com

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摘要：

背景：细胞在支架上的生长行为受到支架孔径及孔隙率等多种因素影响。
目的：观察在体外中空钛合金假体壁上孔洞直径对骨髓基质干细胞生长及分化的影响。
方法：将中空多孔金属试件按照壁上及底面孔径大小分为：φ1：1 mm；φ2：1.5 mm两组，均置于骨髓基质干细胞悬液中进行成骨诱导培养，以未成骨诱导培养作对照。分别于2，4周倒置相差显微镜观察细胞形态、生长情况，成骨诱导钙结节结果。
结果与结论：4周时φ1实验组孔洞中细胞相向生长并相连，φ2组孔洞中未见相连现象；成骨诱导组在第4周有明显的钙结节形成。表明在体外骨髓基质干细胞在适当的钛合金金属孔径内可直接相向生长并联结，以预防植入物-宿主骨潜在间隙的形成；同时这种空间构型可为再生骨提供足够的生长空间。

关键词: 中空试件, 孔径, 骨髓基质干细胞, 生长方式, 钛合金, 生物材料

Abstract:

BACKGROUND: The growth behavior of cells on a stent is influenced by the pore size and porosity of stents as well as many other factors.
OBJECTIVE: To observe the effect of different pore sizes of hollow titanium alloy prosthesis’s wall on the growth of bone marrow stromal cells (BMSCs).
METHODS: Hollow porous metal prostheses were divided into two groups by the pore size of the wall, which were φ1: 1 mm and φ2: 1.5 mm. The prosthesis was located in the BMSCs suspension for osteogenic induction. BMSCs without osteogenic induction served as controls. The cell morphology, growth and calcium nodules were observed under inverted phase contrast microscope.
RESULTS AND CONCLUSION: At 4 week, BMSCs were linked directly through the hole of the groupφ1, but not in the groupφ2. Calcium nodules were formed in the groupsφ1 andφ2. These indicate that BMSCs cultured in vitro can be linked directly in the proper pore size to prevent a potential gap between implants and the host bone. In addition, this spatial structure can provide sufficient space for the growth of regenerated bones.

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