中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6871-6875.doi: 10.3969/j.issn.2095-4344.2012.37.007

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

兔椎间盘退变模型建立及骨形态发生蛋白7的表达

李刚建1，赵 鑫2   

  1. 1浦东新区光明中医院骨科，上海市 201300
    2上海交通大学附属第九人民医院骨科，上海市 201360
  • 收稿日期:2012-05-12 修回日期:2012-06-21 出版日期:2012-09-09 发布日期:2012-09-09
  • 作者简介:李刚建，男，1973年生，上海市人，汉族，1997年北京中医药大学毕业，主治医师。主要从事四肢创伤及脊柱骨科临床研究。 ligangjian666@hotmail.com

Construction of an animal model of intervertebral disc degeneration and expression of bone morphogenetic protein-7

Li Gang-jian1, Zhao Xin2   

  1. 1Department of Orthopedics, Guangming Hospital of Traditional Chinese Medicine, Shanghai 201300, China
    2Department of Orthopedics, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201360, China
  • Received:2012-05-12 Revised:2012-06-21 Online:2012-09-09 Published:2012-09-09
  • About author:Li Gang-jian, Attending physician, Department of Orthopedics, Guangming Hospital of Traditional Chinese Medicine, Shanghai 201300, China ligangjian666@hotmail.com

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被引次数

1

摘要：

背景：骨形态发生蛋白7可以促进椎间盘组织合成细胞外基质。
目的：观察椎间盘退变兔模型骨形态发生蛋白7的表达。
方法：新西兰大白兔采用髓核抽吸法建立椎间盘退变动物模型，对照组暴露相应椎间隙后不进行任何干预。建模成功后，连续核磁共振观察椎间盘变化，12周后生化分析椎间盘中蛋白多糖含量，同时免疫组织化学检测退变的椎间盘组织中Ⅱ型胶原和骨形态发生蛋白7的表达。
结果与结论：建模后2周，核磁共振发现模型兔椎间盘开始退变，出现椎间隙狭窄，T2像上椎间盘信号强度减弱。建模后12周，模型兔椎间隙高度、T2信号强度、蛋白多糖含量、Ⅱ型胶原和骨形态发生蛋白7的表达均低于对照组(P < 0.05)。结果证实，实验采用髓核抽吸法成功建立了退变椎间盘兔模型，其退变的椎间盘组织中骨形态发生蛋白7的表达下降。

关键词: 椎间盘退变, 腰椎, 骨形态发生蛋白7, 蛋白多糖, Ⅱ型胶原, 髓核抽吸法, 组织构建

Abstract:

BACKGROUND: Bone morphogenetic protein-7 can promote extracellular matrix synthesis in the intervertebral disc tissue.
OBJECTIVE: To observe the expression of bone morphogenetic protein-7 in rabbits with intervertebral disc degeneration.
METHODS: Models of intervertebral disc degeneration were established in New Zealand white rabbits using nucleus aspiration. The lumbar intervertebral disc of L3/4, L4/5, L5/6 were exposed in control group without treatment. The changes in the intervertebral disc were observed by MRI after modeling. The contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen were measured in 12 weeks later.
RESULTS AND CONCLUSION: Intervertebral disc degeneration occurred at 2 weeks after modeling detected by using MRI. Compared with the control group, the disc height, T2 value, contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen in the model group were significantly decreased at 12 weeks after modeling. It indicated that the expression of bone morphogenetic protein-7 was declined in the degenerative intervertebral disc of rabbits induced by nucleus aspiration method.

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