中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (34): 6354-6358.doi: 10.3969/j.issn.2095-4344.2012.34.018

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

RT-Q医用生物膜的生物相容性评价

申 理，王春梅，赵 毅，郑明武，李 岩，陈重华   

  1. 四川大学华西药学院药理教研室，四川省成都市 610041
  • 收稿日期:2012-01-15 修回日期:2012-01-15 出版日期:2012-08-19 发布日期:2012-08-19
  • 通讯作者: 陈重华，硕士，副教授，四川大学华西药学院药理教研室，四川省成都市 610041 chenzhonghua-1@163.com
  • 作者简介:申理★，男，1983年生，陕西省西安市人，汉族，四川大学华西药学院在读硕士，主要从事复方制剂的药效学评价与新药药理研究。 sljsljsljslj@ 163.com

Biocompatibility of RT-Q medical biomembrane

Shen Li, Wang Chun-mei, Zhao Yi, Zheng Ming-wu, Li Yan, Chen Zhong-hua   

  1. Department of Pharmacology, West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Received:2012-01-15 Revised:2012-01-15 Online:2012-08-19 Published:2012-08-19
  • Contact: Chen Zhong-hua, Master, Associate professor, Department of Pharmacology, West China School of Pharmacy, Sichuan University, Chengdu 610041, Sichuan Province, China chenzhonghua-1@ 163.com
  • About author:Shen Li★, Studying for master’s degree, Department of Pharmacology, West China School of Pharmacy, Sichuan University, Chengdu 610041,Sichuan Province, China sljsljsljslj@163.com

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被引次数

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摘要：

背景：有研究表明RT-Q医用生物膜局部止血效果明显，具有一定的组织可相容性。
目的：考查RT-Q医用生物膜的主要生物相容性指标。
方法：①MTT法考查0(阴性对照)，12.5%，25%，50%，100% RT-Q医用生物膜浸取液对L2929细胞的毒性。②将16只兔随机分为阴性对照组、阳性对照组、完整皮肤组、破损皮肤组。前3组分别于脊柱右侧皮肤喷涂生理盐水、甲醛及RT-Q医用生物膜，破损皮肤组在脊柱两侧划破表皮后于右侧喷涂RT-Q医用生物膜；各组左侧均喷涂生理盐水。③划破12只兔耳缘静脉后，分别于伤口处涂喷RT-Q医用生物膜(实验组)及注射生理盐水(对照组)。④在30只豚鼠左侧背部皮肤分别喷涂RT-Q医用生物膜(实验组)、生理盐水(阴性对照组)及2,4-二硝基氯苯(阳性对照组)。
结果与结论：各浓度RT-Q医用生物膜对L2929细胞无毒性。RT-Q医用生物膜对动物完整皮肤和破损皮肤及破损血管无刺激性，未见过敏反应，具有止血效果。表明RT-Q医用生物膜主要生物相容性评价指标合格，生物相容性良好。

关键词: RT-Q医用生物膜, 生物相容性, 细胞毒性, 刺激性, 过敏性, 生物材料

Abstract:

BACKGROUND: Early studies have shown that RT-Q medical biomembrane has a significant hemostatic effect on topical bleeding, and possesses good histocompatibility.
OBJECTIVE: To investigate the main biocompatibility indices of RT-Q medical biomembrane.
METHODS: First, MTT method was used to determine the cytotoxicity of 0 (control), 12.5%, 25%, 50% and 100% RT-Q medical biomembrane on L2929 cells. Second, 16 rabbits were randomly divided into negative control group, positive control group, intact skin group and broken skin group. Rabbits in the former three groups were sprayed with normal saline, formaldehyde and RT-Q medical biomembrane, respectively, on the right side of the spine skin. While, rabbits in the broken skin group were only sprayed with RT-Q medical biomembrane on the right side of the spine skin after piercing in the bilateral spine skin. Next, the 12 rabbits were sprayed with RT-Q medical biomembrane and injected with normal saline after piercing the vain of rabbit’s ear border. Last, 30 Guinea pigs were sprayed with RT-Q medical biomembrane, normal saline and 2,4-dinitrochlorobenzene, respectively.
RESULTS AND CONCLUSION: In cytotoxicity test, various concentrations of RT-Q medical biomembrane showed non-toxicity to L2929 cells. Besides, RT-Q medical biomembrane was nonirritant to the intact and broken skin of animals as well as broken vessels. In addition, there were no allergic reactions and it had the hemostatic effect. These results suggest that RT-Q medical biomembrane have passed the main tests of biocompatibility for its good biocompatibility.

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