中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 6032-6036.doi: 10.3969/j.issn.2095-4344.2012.32.027

• 干细胞因子及调控因子 stem cell factors and regulatory factors • 上一篇    下一篇

右归丸联合碱性成纤维细胞生长因子诱导大鼠骨髓基质细胞分化为 神经元样细胞

杨 玲1，张作涛1，张力华2，陆 莹1   

  1. 1贵阳中医学院解剖教研室，贵州省贵阳市 550002；
    2成都中医药大学解剖与组胚教研室，四川省成都市 610075
  • 收稿日期:2012-05-02 修回日期:2012-06-07 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 张力华，教授，成都中医药大学解剖与组胚教研室，四川省成都市610075 848774944@qq.com
  • 作者简介:杨玲★，女，1983年生，贵州省六盘水市人，汉族，2011年成都中医药大学毕业，硕士，助教，主要从事成体干细胞应用基础研究。 89688173@qq.com

Yougui Wan combined with basic fibroblast growth factors induce the differentiation of rat bone marrow stromal cells into neuron-like cells

Yang Ling1, Zhang Zuo-tao1, Zhang Li-hua2, Lu Ying1   

  1. 1Department of Human Anatomy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China;
    2Department of Human Anatomy and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China
  • Received:2012-05-02 Revised:2012-06-07 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Li-hua, Professor, Department of Human Anatomy and Embryology, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China 848774944qq.com
  • About author:Yang Ling★, Master, Department of Human Anatomy, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China 89688173.qq.com.

PDF

381

被引次数

3

摘要：

背景：既往研究证实补肾益精中药能体外诱导骨髓基质细胞转化为神经元样细胞。
目的：观察右归丸联合碱性成纤维细胞生长因子体外诱导大鼠骨髓基质细胞向神经元样细胞的分化作用。
方法：密度梯度离心法结合贴壁培养法培养大鼠骨髓基质细胞，将传3代的骨髓基质细胞用右归丸联合碱性成纤维细胞生长因子诱导向神经元样细胞分化，并设单独碱性成纤维细胞生长因子组和正常组为对照。
结果与结论：诱导7 d后，光学显微镜下观察右归丸联合碱性成纤维细胞生长因子诱导组部分细胞呈双极或多极神经元形态。免疫组织化学染色结果显示，与正常组和单独碱性成纤维细胞生长因子组相比，右归丸+碱性成纤维细胞生长因子组细胞中神经元特异性醇化酶、巢蛋白和胶质纤维酸性蛋白阳性率明显增高(P < 0.05)。结果说明，右归丸联合碱性成纤维细胞生长因子可体外诱导骨髓基质细胞分化为神经元样细胞。

关键词: 右归丸, 骨髓基质细胞, 诱导分化, 神经元样细胞, 碱性成纤维细胞生长因子, 中医药, 干细胞

Abstract:

BACKGROUND: Previous studies have demonstrated that Chinese medicine for tonifying kidney and benefiting essence can induce bone marrow stromal cells to differentiate into neuron-like cells.
OBJECTIVE: To investigate the differentiation of bone marrow stromal cells into neuron-like cells under the induction of Yougui Wan combined with basic fibroblast growth factor.
METHODS: Bone marrow stromal cells were isolated by attachment and density centrifugation. Passage 3 bone marrow stromal cells were induced to differentiate into neuron-like cells by Yougui Wan combined with basic fibroblast growth factor. Bone marrow stromal cells were divided into three groups: (1) basic fibroblast growth factor group was induced by basic fibroblast growth factor; (2) Yougui Wan+basic fibroblast growth factor group was induced by Yougui Wan and basic fibroblast growth factor; and (3) control group received no administration.
RESULTS AND CONCLUSION: After 7 days of induction, some cells showed typical bipolar and multipolar neuronal morphology. Immunohistochemical staining results showed that neuron specific endolase, nestin and glial fibrillary acidic protein expressions in the Yougui Wan + basic fibroblast growth factor group were significantly greater than in the basic fibroblast growth factor group and control group (P < 0.05). These findings suggest that Yougui Wan combined with basic fibroblast growth factor can induce bone marrow stromal cells to differentiate into neuron-like cells.

中图分类号: