中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5941-5945.doi: 10.3969/j.issn.2095-4344.2012.32.010

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

人颊脂垫脂肪干细胞与皮下脂肪干细胞生物学特性的比较

张圣敏，陈 刚，王晨星，高振杰，乔 峰   

  1. 天津医科大学口腔医院颌面外科，天津市 300070
  • 收稿日期:2011-12-20 修回日期:2011-12-20 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 陈刚，博士，教授，主任医师，天津医科大学口腔医院颌面外科，天津市 300070 doctorchen@live.cn
  • 作者简介:张圣敏★，女，1985年生，河北省沧州市人，汉族，天津医科大学在读硕士，主要从事口腔颌面外科研究。 onlydoctorzsm@163.com

Biological characteristics of adipose stem cells from buccal fat pad and traditional liposuction

Zhang Sheng-min, Chen Gang, Wang Chen-xing, Gao Zhen-jie, Qiao Feng   

  1. Department of Maxillofacial Surgery, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China
  • Received:2011-12-20 Revised:2011-12-20 Online:2012-08-05 Published:2012-08-05
  • Contact: Chen Gang, M.D., Professor, Chief physician, Department of Maxillofacial Surgery, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China doctorchen@live.cn
  • About author:Zhang Sheng-min★, Studying for master’s degree, Department of Maxillofacial Surgery, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China onlydoctorzsm@163.com

PDF

340

被引次数

9

摘要：

背景：在人脂肪干细胞的研究中，针对其内脏脂肪与皮下脂肪生物学特性的比较较多，对人颊脂垫部位脂肪干细胞的研究较少。
目的：提取人颊脂垫脂肪干细胞进行体外增殖及成骨诱导培养，观察其细胞生物学特性，并与皮下脂肪来源脂肪干细胞进行对比。
方法：分别提取面部轮廓整形手术获取的人颊脂垫与皮下吸脂术获取的皮下脂肪中的脂肪干细胞，进行体外传代培养与成骨诱导，对二者细胞浓度、增殖活性、成骨能力等指标进行对比观察。
结果与结论：人颊脂垫为特化脂肪组织，血管成分明显多于皮下脂肪。颊脂垫提取脂肪干细胞浓度显著高于吸脂术来源者；CCK-8实验生长曲线显示颊脂垫来源脂肪干细胞增殖活性亦高于吸脂术来源脂肪干细胞(P < 0.05)；经成骨培养后第3，7，14天碱性磷酸酶染色，颊脂垫来源脂肪干细胞成骨活性优于吸脂来源脂肪干细胞(P < 0.05)。表明颊脂垫作为特化的深部脂肪组织，其包含的脂肪干细胞生物学特性明显优于吸脂来源者，且由于其解剖毗邻关系，是口腔颌面部骨组织工程理想的种子细胞库。

关键词: 颊脂垫, 脂肪干细胞, 细胞增殖, 细胞培养, 成骨, 干细胞

Abstract:

BACKGROUND: Most of human adipose-derived stem cells studies describe the biological characteristics of subcutaneous fat and visceral fat. There are few studies focusing on buccal fat pad.
OBJECTIVE: To extract human buccal fat pad adipose-derived stem cells for in vitro proliferation and osteogenic induction and compare the biological characteristics of adipose stem cells from buccal fat pad and traditional liposuction.
METHODS: Adipose-derived stem cells were harvested from buccal fat pad and traditional liposuction. Then cells were in vitro cultured and induced for osteogenic differentiation. Cell density, proliferation activity and osteogenic capacity were compared between these two kinds of cells.
RESULTS AND CONCLUSIONS: Human buccal fat pad exhibited more blood vessels than subcutaneous fat. Higher densities of adipose-derived stem cells were obtained from buccal fat pad than traditional liposuction. CCK-8 test showed that the proliferation activity of adipose-derived stem cells from buccal fat pad was significantly higher than that from traditional liposuction (P < 0.05). After osteogenic culture for 3, 7, 14 days, alkaline phosphatase activity was significantly greater in the adipose-derived stem cells from buccal fat pad than that from the traditional liposuction (P < 0.05). These findings suggest that the biological characteristics of adipose-derived stem cells from the buccal fat pad, a special deep fat tissue, are superior to those of adipose-derived stem cells from the traditional liposuction. Moreover, because of its close anatomical relationship to oral maxillofacial region, buccal fat pad can be used as a potential ideal seed cell source for bone tissue engineering in the oromaxillo facial region.

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