中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5936-5940.doi: 10.3969/j.issn.2095-4344.2012.32.009

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

成人再生障碍性贫血患者骨髓间充质干细胞增殖分化潜能： 与健康人的比较

  

  1. 哈尔滨市第一医院血液病肿瘤研究所，黑龙江省哈尔滨市 150010
  • 收稿日期:2011-12-04 修回日期:2011-12-04 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 张颢，副主任医师，哈尔滨市第一医院血液病肿瘤研究所，黑龙江省哈尔滨市 150010 gx-zhanghao@126.com
  • 作者简介:程梅★，女，1981年生，山东省曹县人，汉族，2009年重庆医科大学毕业，硕士，助理研究员，主要从事间充质干细胞生物学特性研究及治疗。 chengmei0451@126.com

Proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells in adult patients with aplastic anemia versus in healthy adults

Cheng Mei, Zhang Hao, Tao Yan-ling, Cheng Huan-chen, Wang Zhi-guo, Chen Bo, Xiao Liang, Xia Guo-qiang, Qiu Lin, Zhan Zhao-min, Zhang Bo-long, Ma Jun   

  1. Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China
  • Received:2011-12-04 Revised:2011-12-04 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Hao, Associate chief physician, Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China gx-zhanghao@126.com
  • About author:Cheng Mei★, Master, Assistant researcher, Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China chengmei0451@126.com

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摘要：

背景：骨髓间充质干细胞具有成脂和成骨分化潜能，再生障碍性贫血患者骨髓间充质干细胞可能存在成脂和成骨分化异常。
目的：观察再生障碍性贫血患者骨髓间充质干细胞增殖及分化潜能与健康人的差异。
方法：选择哈尔滨市第一医院血液病肿瘤研究所收治的再生障碍性贫血患者5例，另选5例健康成人作为对照组；采用全骨髓培养法分离骨髓间充质干细胞。
结果与结论：采用全骨髓培养法成功分离出骨髓间充质干细胞；与对照组相比，再生障碍性贫血组骨髓间充质干细胞的细胞形态和免疫表型无显著差异，皆表达CD73、CD90、CD105，不表达CD34、CD45和HLA-DR；再生障碍性贫血组骨髓间充质干细胞增殖能力显著低于对照组(P < 0.05)，成脂肪细胞分化诱导率高于对照组(P < 0.05)，PPARγ mRNA和FABP4 mRNA表达量均高于对照组(P < 0.05)；而成骨细胞分化诱导率低于正常对照组(P < 0.05)，ALP mRNA和BGLAP mRNA表达量分别低于对照组(P < 0.05)。提示再生障碍性贫血组骨髓间充质干细胞分化潜能存在成脂肪细胞和成骨细胞分化的失衡。

关键词: 再生障碍性贫血, 骨髓间充质干细胞, 成脂肪细胞, 成骨细胞, 生物特性, 干细胞

Abstract:

BACKGROUND: Bone marrow-derived mesenchymal stem cells (BMSCs) have adipogenic and osteogenic differentiation potential. BMSCs in patients with aplastic anemia (AA) may suffer from adipogenic and osteogenic differentiation abnormality.
OBJECTIVE: To compare the proliferation and differentiation potential of BMSCs in AA patients versus in healthy adults.
METHODS: AA group included five adult patients who received treatment in the Institute of Hematology & Oncology, Harbin First Hospital. Control group consisted of five healthy adults. BMSCs were isolated by whole bone marrow culture method.
RESULTS AND CONCLUSION: BMSCs were isolated and purified successfully and effectively. Morphology and immunophenotype of BMSCs from AA group showed no significant difference compared with control group. Two groups were all positive for MSC-related antigens, such as CD73, CD90, CD105, but negative for CD34, CD45, and HLA-DR. Compared with the control group, BMSC proliferation was significantly lower (P < 0.05), the frequency of adipocyte differentiation was significantly higher (P < 0.05), PPARγ mRNA and FABP4 mRNA expression was significantly higher (P < 0.05), the frequency of osteoblast differentiation was significantly lower (P < 0.05), and ALP mRNA and BGLAP mRNA expression was significantly decreased (P < 0.05) in the AA group. These findings suggest that BMSCs from AA patients exhibit unbalanced differentiation potential of osteogenesis and adipocyte.

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