中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (28): 5222-5226.doi: 10.3969/j.issn.2095-4344.2012.28.019

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

高尿酸血症对大鼠主动脉平滑肌细胞Ki-67及尿酸盐阴离子交换器 表达的影响

宋 飞1, 2，王新美3，赵文娟1   

  1. 1青岛大学医学院附属医院内分泌科，山东省青岛市 266003；
    2山东省淄博市临淄区妇幼保健院，山东省淄博市 255400；
    3淄博市中心医院病理科，山东省淄博市 255036
  • 收稿日期:2011-12-03 修回日期:2011-12-22 出版日期:2012-07-08 发布日期:2012-07-08
  • 通讯作者: 赵文娟，主任医师，青岛大学医学院附属医院内分泌科，山东省青岛市 266003 zhaowenjuan@medmail.com.cn
  • 作者简介:宋飞★，女，1976年生，汉族，山东省德州市人，2000年滨州医学院毕业，主治医师，在读硕士，主要从事代谢综合症相关疾病研究。wbjsf@126.com

Effect of hyperuricemia on the expression of Ki-67 in rat aortic and urate anion exchanger smooth muscle cells

Song Fei1, 2, Wang Xin-mei3, Zhao Wen-juan1   

  1. 1Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China;
    2Linzi Maternity and Child Care Hospital of Shandong, Zibo 255400, Shandong Province, China;
    3Deparment of Pathology, Zibo Central Hospital, Zibo 255036, Shandong Province, China
  • Received:2011-12-03 Revised:2011-12-22 Online:2012-07-08 Published:2012-07-08
  • Contact: Zhao Wen-juan, Chief physician, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China zhaowenjuan@medmail.com.cn
  • About author:Song Fei★, Studying for master’s degree, Attending physician, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China; Linzi Maternity and Child Care Hospital of Shandong, Zibo 255400, Shandong Province, China wbjsf@126.com

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480

被引次数

1

摘要：

背景：尿酸盐阴离子交换器在调节全身的尿酸盐稳态中起重要作用，Ki-67是细胞增殖的重要标志物，那么二者对高尿酸血症大鼠主动脉平滑肌细胞有何影响呢？
目的：观察高尿酸血症对大鼠主动脉平滑肌细胞Ki-67及尿酸盐阴离子交换器表达的影响。
方法：20只8周龄雄性Wistar大鼠随机摸球法均分为实验组和对照组。实验组给予高酵母饲料喂养，同时给予腺嘌呤溶液灌胃，对照组给予普通饮食及同体积蒸馏水灌胃。当血尿酸水平出现差异时，继续饲养5周处死所有大鼠，苏木精伊红染色观察主动脉平滑肌组织结构，免疫组化检测胸主动脉平滑肌细胞增殖核抗原Ki-67以及尿酸盐阴离子交换器的表达。
结果与结论：罹患5周高尿酸血症对16周龄Wistar大鼠主动脉平滑肌细胞Ki-67及尿酸盐阴离子交换器表达差异无显著性意义，也未造成动脉粥样硬化样改变。说明高尿酸血症作为动脉硬化的影响因素之一，可能需要更长期的病程才可能体现出来。

关键词: 高尿酸血症, 主动脉平滑肌增殖, Ki-67, URAT1, 细胞增殖

Abstract:

BACKGROUND: Urate anion exchanger (URAT1) plays an important role in adjusting steady-state of urate in the whole body, and ki-67 is an important cell multiplication marker. What is their effect on the aortic smooth muscle cells (ASMCs) of hyperuricemia rats?
OBJECTIVE: To observe the effect of hyperuricemia on the expression of Ki-67 in ASMCs and URAT1.
METHODS: Twenty 8-week-old Wistar rats were randomly divided into two groups: control group (n=10) and experimental group (n=10). Rats in the experimental group were fed with high yeast and gavaged with amidopurine fluid. However, rats in the control group were fed with general diet and gavaged with the same dose of distilled water. When the level of blood uric acid started to show the difference, all the rats were fed for another 5 weeks and then were sacrificed. Hematoxylin-eosin staining was used to observe the structure of aortic smooth muscle and immunohistochemistry was used to detect the expression of Ki-67 in ASMCs and URAT1.
RESULTS AND CONCLUSION: Hyperuricemia that persisted for 5 weeks had no obvious effect on the expression of Ki-67 and in ASMCs and URAT1 in 16-week-old male Wistar rats, or did not result in atherosclerosis. As an influential factor of atherosclerosis, hyperuricemia needs more time to result in atherosclerosis.

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