中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (26): 4148-4153.doi: 10.3969/j.issn.2095-4344.1355

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

纳米羟基磷灰石/胶原复合材料修复猪下颌骨缺损后血管内皮生长因子的变化

李冬梅1，刘新晖2，李庆星1
  

  1. 1河北医科大学第一医院口腔科，河北省石家庄市  050031；2南京市江宁医院骨科，江苏省南京市  211100
  • 收稿日期:2019-03-12
  • 通讯作者: 李庆星，河北医科大学第一医院口腔科，河北省石家庄市 050031
  • 作者简介:李冬梅，女，1970年生，河北省阳原县人，副主任医师，主要从事口腔疾病诊治工作。
  • 基金资助:

    河北省医学科学研究指导课题计划项目(08069)，课题名称：新型纳米生物材料修复下颌骨骨缺损的动物实验研究，项目负责人：李冬梅

Changes of vascular endothelial growth factor after repair of pig mandibular defects by nano-hydroxyapatite/collagen composite

Li Dongmei1, Liu Xinhui2, Li Qingxing1 
  

  1. 1Department of Stomatology, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China; 2Department of Orthopedics, Nanjing Jiangning Hospital, Nanjing 211100, Jiangsu Province, China
  • Received:2019-03-12
  • Contact: Li Qingxing, Department of Stomatology, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • About author:Li Dongmei, Associate chief physician, Department of Stomatology, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • Supported by:

    the Medical Science Research Guidance Program of Hebei Province, No. 08069 (to LDM)

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摘要：

文章快速阅读：

 

文题释义：
胶原的生物学功能：是构成细胞外基质的骨架，能与细胞相互作用，影响细胞的形态、分化及增殖；免疫原性相对较低，生物相容性较好，有助于促进细胞的增殖及生物降解；能与周围基质相互影响，成为细胞与组织正常生理功能的重要组成部分；具备可降解性，能促进细胞代谢。
血管内皮生长因子：又称血管通透因子，是一种高度特异性的促血管内皮细胞生长因子，具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形成等作用。
 
 
背景：纳米羟基磷灰石/胶原复合材料可促进下颌骨缺损的修复，但其作用机制尚不明确。
目的：探讨纳米羟基磷灰石/胶原复合材料对家猪下颌骨缺损中血管内皮生长因子的影响。
方法：取家猪60只(河北医科大学第一医院医学动物实验中心提供)，随机分3组，每组20只：假手术组不做任何处理；模型对照组建立10 mm×5 mm×1 mm下颌骨缺损模型；观察组建立10 mm×5 mm×1 mm下颌骨缺损模型，将纳米羟基磷灰石/胶原复合材料植入骨缺损处。术后12周，取下颌骨缺损部位，分别进行苏木精-伊红染色、免疫组织化学染色、X射线检查。实验获得河北医科大学第一医院伦理委员会批准。
结果与结论：①苏木精-伊红染色显示，观察组材料中心区部分材料降解，大量代谢旺盛的细胞分散在材料空洞中，周围可见大量骨小梁；模型对照组缺损区域被纤维肉芽组织覆盖，未见明显成骨发生；假手术组下颌骨部位组织排列整齐；观察组新生骨小梁面积大于模型对照组(P < 0.05)；②X射线显示，模型对照组可见明显缺损部位；观察组缺损面积明显小于模型对照组；③免疫组织化学染色显示，观察组血管内皮生长因子表达高于模型对照组(P < 0.05)；④结果表明，纳米羟基磷灰石/胶原复合材料可促进猪下颌骨缺损的愈合，提高血管内皮生长因子水平。

关键词: 纳米羟基磷灰石/胶原复合材料, 下颌骨缺损, 血管内皮生长因子, 新生骨小梁面积, 组织学观察, 骨缺损, 血管形成, 免疫组织化学

Abstract:

BACKGROUND: Nano-hydroxyapatite/collagen composites can promote the repair of mandibular defects, but the mechanism is still unclear.
OBJECTIVE: To investigate the effect of nano-hydroxyapatite/collagen composites on vascular endothelial growth factors in mandibular defects of domestic pigs.
METHODS: Sixty domestic pigs (provided by the Laboratory Animal Center of the First Hospital of Hebei Medical University, China) were randomized into three groups (n=20/group): sham group (no intervention), control group (model of mandibular defect with 10 mm×5 mm×1 mm), and observation group (model of mandibular defect with 10 mm×5 mm×1 mm, and implantation of nano-hydroxyapatite/collagen composite material). At 12 weeks after surgery, the mandibular defect areas were removed for hematoxylin-eosin staining, immunohistochemistry and X-ray examination. The study was approved by the Animal Ethics Committee of the First Hospital of Hebei Medical University.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that some materials in the central part of the material in the observation group were degraded, and a large number of metabolically active cells were dispersed in the material cavity, which was surrounded by numerous trabecular bones. The defect area in the control group was covered by fibrous granulation tissue without obvious osteogenesis. The tissues in the mandibular part of sham group arranged regularly. The area of newly born bone in the observation group was significantly larger than that in the control group (P < 0.05). X-ray examination revealed that there was obvious defect in the control group, and the defect area in the observation group was significantly smaller than that in the control group. Immunohistochemistry results indicated that the expression level of vascular endothelial growth factor in the observation group was significantly higher than that in the control group (P < 0.05). In summary, nano-hydroxyapatite/collagen composite can promote the healing of pig mandibular defect, and increase vascular endothelial growth factor level.

Key words: nano-hydroxyapatite/collagen composite, mandibular defect, vascular endothelial growth factor, new trabecular bone area, histological observation, bone defects, angiogenesis, immunohistochemistry

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