中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (22): 3570-3576.doi: 10.3969/j.issn.2095-4344.1283

• 生物材料综述 biomaterial review • 上一篇    下一篇

关节软骨细胞外基质代谢中Sox9的调控作用

房  鹏,赵建宁,张  雷
  

  1. 南京大学医学院附属金陵医院骨科,江苏省南京市   210002
  • 收稿日期:2019-02-27
  • 通讯作者: 张雷,博士,主治医师,南京大学医学院附属金陵医院骨科,江苏省南京市 210002
  • 作者简介:房鹏,男,1992生,安徽省池州市人,汉族,南京大学在读硕士,主要从事关节软骨损伤修复的研究。
  • 基金资助:

    国家自然科学基金青年科学基金项目(81702170),项目负责人:张雷;江苏省自然科学基金青年基金项目(BK20170624),项目负责人:张雷;中国博士后科学基金特别资助项目(2017T00826),项目负责人:张雷

Regulatory role of Sox9 in metabolism of articular cartilage extracellular matrix

Fang Peng, Zhao Jianning, Zhang Lei
  

  1. Department of Orthopedics, Jinling Hospital of Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China
  • Received:2019-02-27
  • Contact: Zhang Lei, MD, Attending physician, Department of Orthopedics, Jinling Hospital of Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China
  • About author:Fang Peng, Master candidate, Department of Orthopedics, Jinling Hospital of Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China
  • Supported by:

    the National Natural Science Foundation of China for the Youth of China, No. 81702170 (to ZL); the Natural Science Foundation of China for the Youth of Jiangsu Province, No. BK20170624 (to ZL); the National Postdoctoral Science Foundation of China, No. 2017T00826 (to ZL)

摘要:

文章快速阅读:

 

文题释义:
骨关节炎:是一种临床上常见的退行性关节疾病,以进行性软骨退变、软骨下骨改变、骨赘形成等为其主要病理特征。骨关节炎的病因复杂,但不外乎软骨细胞和其细胞外基质的改变。细胞外基质作为软骨细胞的内环境在软骨退变中扮演着重要的角色。
Sox9:属于Sox(Sry-type HMG box)蛋白家族,是细胞内的重要转录因子。其在关节软骨细胞外基质代谢方面起着重要的调节作用,可上调Ⅱ型胶原的表达。
 
 
背景:软骨细胞外基质在关节退变中扮演着角色。Sox9是细胞内的重要转录因子,其在关节软骨细胞外基质代谢方面起着重要的调节作用。Sox9能够促进软骨标志物Ⅱ型胶原、蛋白聚糖和Ⅸ型胶原的基因转录,并且能够抑制聚蛋白多糖酶、基质金属蛋白酶的表达,从而维持关节软骨细胞表型。Sox9在骨关节炎早期代偿性表达上调,随着病情的进展Sox9表达下调,因此Sox9是骨关节炎进行性发展的重要靶点。
目的:综述Sox9对于关节软骨细胞外基质代谢的作用。
方法:第一作者应用计算机检索PubMed和中国知网数据库中关于Sox9的文献,在关键词中以“Sox9,骨关节炎,Ⅱ型胶原,X型胶原,基质金属蛋白酶,聚蛋白多糖酶”为中文检索词,以“Sox9,Osteoarthritis,collagen type II,collagen type X,MMPs,ADAMTS”为英文检索词进行检索。选择65篇文献进行综述。
结果与结论:①Sox9是软骨细胞的“主调控因子”,是维持软骨细胞表型和软骨稳态的关键转录因子之一,但同时其表达受到精确的调控;②Sox9基因表达调控的具体机制十分复杂,如何上调软骨细胞中Sox9基因的表达有望成为预防和治疗骨关节炎的重要方向之一。

关键词: Sox9, 骨关节炎, 关节软骨, 软骨细胞, 细胞外基质, 代谢, 国家自然科学基金

Abstract:

BACKGROUND: Extracellular matrix of chondrocytes plays a role in joint degeneration. Sox9 is an important transcription factor in cells, and plays an important regulatory role in the metabolism of articular cartilage extracellular matrix. It can promote the gene transcription of cartilage markers: type II collagen, proteoglycan and type IX collagen, and inhibit the expression of aggrecanase and matrix metalloproteinase, thereby maintaining the chondrocyte phenotype. Sox9 is up-regulated in compensatory expression in the early stage of osteoarthritis, and down-regulated as the disease progresses. Therefore, Sox9 is an important target for the progressive development of osteoarthritis.

OBJECTIVE: To review the role of Sox9 in the metabolism of chondrocyte extracellular matrix.

METHODS: A computer-based search was performed in the PubMed and CNKI databases for articles addressing Sox9. The keywords were “Sox9, osteoarthritis, collagen type II, collagen type X, MMPs, ADAMTS” in English and Chinese, respectively. Finally, 65 eligible articles were included to review.

RESULTS AND CONCLUSION: (1) Sox9 is the main regulatory factor of chondrocytes and one of the key transcription factors to maintain chondrocyte phenotype and cartilage homeostasis. (2) However, the specific mechanism for the regulation of Sox9 gene expression is very complex at present. How to up-regulate the expression of Sox9 gene in chondrocytes is expected to become an important direction for the prevention and treatment of osteoarthritis

Key words: Sox9, osteoarthritis, articular cartilage, chondrocytes, extracellular matrix, metabolism, the National Natural Science Foundation of China

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