中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (26): 4184-4190.doi: 10.3969/j.issn.2095-4344.0914

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

钙磷涂层镁合金支架的抗腐蚀性及生物相容性

马 飞1，李 想1，谢瑞敏1，王勇平1，何耀华2   

  1. 1兰州大学第一医院骨科，甘肃省兰州市 730000；2上海交通大学第六人民医院骨科，上海市 200233
  • 收稿日期:2018-04-08
  • 通讯作者: 王勇平，博士、博士后，研究生导师，兰州大学第一医院骨科，甘肃省兰州市 730000
  • 作者简介:马飞，男，1986年生，甘肃省陇南市人，汉族，兰州大学第一临床医学院在读硕士，主治医师，主要从事骨科的创伤、脊柱及骨科生物材料研究。
  • 基金资助:

    甘肃省高等学校科研项目(2018B-013)；兰州大学第一医院科研项目(ldyyyn2017-21；ldyyyn2013-01)；国家自然科学基金资助项目(81271961，81572106)

Effect of calcium phosphate coatings on corrosion resistance and biocompatibility of magnesium alloy scaffolds

Ma Fei1, Li Xiang1, Xie Rui-min1, Wang Yong-ping1, He Yao-hua2   

  1. 1兰州大学第一医院骨科，甘肃省兰州市 730000；2上海交通大学第六人民医院骨科，上海市 200233
  • Received:2018-04-08
  • Contact: Wang Yong-ping, M.D., Master’s supervisor, Department of Orthopedics, First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
  • About author:Ma Fei, Master candidate, Attending physician, Department of Orthopedics, First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China
  • Supported by:

    the Scientific Research Program of Gansu Provincial Universities, No. 2018B-013; the Scientific Research Project of the First Hospital of Lanzhou University, No. ldyyyn2017-21, ldyyyn2013-01; the National Natural Science Foundation of China, No. 81271961, 81572106

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摘要：

文章快速阅读：

 

文题释义：
钙磷涂层：钙磷涂层的化学成分主要由氧、碳、钙和磷构成，具有良好的生物相容性；钙磷涂层可提高镁合金的耐腐蚀性能；钙磷涂层没有明显的细胞毒性，可促进细胞黏附和增殖，可提高细胞的生物活性。
镁合金：是以镁为基础加入其他元素组成的合金，具有可降解性，良好的生物相容性，良好的力学性能，可促进骨细胞生长，诱导成骨细胞和骨细胞的产生。
 
 
背景：镁合金具有良好的生物相容性，可促进成骨细胞生长，然而镁合金在生理环境中的腐蚀速率较高，不能满足临床需要。
目的：探讨钙磷涂层对AZ31镁合金支架抗腐蚀性和生物相容性的影响。
方法：采用化学沉积法在AZ31镁合金支架表面制备钙磷涂层。①静态浸泡实验：将钙磷涂层和无涂层AZ31镁合金支架分别浸泡于DMEM细胞培养液中，浸泡第10，20，30天后检测材料腐蚀速率；②细胞毒性实验：分别以100%，75%，50%，25%浓度的钙磷涂层AZ31镁合金支架浸提液培养MC3T3-E1细胞，培养1，3，5 d，检测细胞增殖率，判定毒性分级；③细胞黏附实验：将MC3T3-E1细胞分别接种于钙磷涂层和无涂层AZ31镁合金支架表面，接种6，12，24 h计数细胞数量；④细胞增殖实验：将MC3T3-E1细胞分别接种于钙磷涂层和无涂层AZ31镁合金支架表面，培养1，3，5 d后检测细胞数；⑤血细胞聚集实验：将钙磷涂层和无涂层AZ31镁合金支架分别浸入抗凝兔血中，8 min后检测红细胞、白细胞及血小板数量。

结果与结论：①钙磷涂层AZ31镁合金支架不同时间点的腐蚀毒率均低于无涂层AZ31镁合金支架(P < 0.05)；②不同浓度钙磷涂层AZ31镁合金支架浸提液的细胞毒性为0至1级；③钙磷涂层AZ31镁合金支架组接种12，24 h的细胞黏附数量多于无涂层AZ31镁合金支架组(P < 0.05)；④钙磷涂层AZ31镁合金支架组培养3，5 d的细胞数量显著多于无涂层AZ31镁合金支架组(P < 0.05)；⑤钙磷涂层AZ31镁合金支架组的红细胞、白细胞及血小板数量与无涂层AZ31镁合金支架组比较无差异；⑥结果表明，钙磷涂层不仅减慢了AZ31镁合金支架的腐蚀速度，而且改善了其生物相容性。

ORCID: 0000-0001-9825-2801(王勇平)

 

关键词: 钙磷涂层, 镁合金, 生物相容性, 抗腐蚀性, 生物材料

Abstract:

BACKGROUND: Magnesium alloy has better biocompatibility and can accelerate growth of osteoblasts, but its  clinical use is limited because of a high corrosion rate in the physiological environment.

OBJECTIVE: To explore the effect of calcium phosphate coating (Ca-P coating) on corrosion resistance and biocompatibility of AZ31 magnesium alloy scaffolds.
METHODS: Using chemical vapor deposition, Ca-P coating was prepared on the surface of AZ31 magnesium alloy scaffolds. (1) Immersion test in static state: AZ31 magnesium alloy scaffolds with or without Ca-P coating were independently immersed in the DMEM medium and the material corrosion rate of each scaffold was tested at 10, 20 and 30 days after immersion. (2) Cytotoxicity test: MC3T3-E1 cells were cultured in the extracts of AZ31 magnesium alloy scaffolds with 100%, 75%, 50% and 25% Ca-P coatings, and cell proliferation and toxicity grade were determined at 1, 3, 5 days after culture. (3) Cell adhesion assay: MC3T3-E1 cells were separately seeded onto the surface of AZ31 magnesium alloy scaffolds with or without Ca-P coating, and cell number was counted at 6, 12 and 24 hours after culture. (4) Cell proliferation test: MC3T3-E1 cells were separately seeded onto the surface of AZ31 magnesium alloy scaffolds with or without Ca-P coating, and cell number was counted at 1, 3, 5 days after culture. (5) Aggregation test of blood cells: AZ31 magnesium alloy scaffolds with or without Ca-P coating were immersed in anticoagulant rabbit blood samples, and amounts of red blood cells, white blood cells and platelets were determined at 8 minutes after immersion.

RESULTS AND CONCLUSION: (1) The corrosion and toxicity rate of AZ31 magnesium alloy scaffold with Ca-P coating was lower than that without Ca-P coating (P < 0.05) without coatings. (2) Cell toxicity of AZ31 magnesium alloy scaffolds with Ca-P coatings of different concentrations was grade 0 to 1. (3) The number of cells adherent to the surface of AZ31 magnesium alloy scaffolds with Ca-P coating was significantly higher than that without Ca-P coating at 12 and 24 hours after inoculation (P < 0.05). (4) The number of cells cultured in the extract of AZ31 magnesium alloy scaffolds with Ca-P coating was significantly higher than that cultured in the extract of the scaffold without Ca-P coating at 3 and 5 days after culture (P < 0.05). (5) The number of red blood cells, white blood cells and platelets in the scaffolds with or without Ca-P coating has no difference (P < 0.05). To conclude, Ca-P coating not only slows the corrosion rate of AZ31 magnesium alloy scaffolds, but also improves the scaffold biocompatibility.

 

Key words: Materials Testing, Corrosion, Cell Adhesion, Cell Proliferation, Tissue Engineering

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