中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (12): 1883-1888.doi: 10.3969/j.issn.2095-4344.0807

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

白细胞介素17A在视神经损伤模型小鼠神经再生中的作用

李艳芬,叶晓阳,陈晓帆,张  伟   

  1. 北京大学,深圳北京大学香港科技大学医学中心,广东省深圳市  518036
  • 收稿日期:2017-11-16 出版日期:2018-04-28 发布日期:2018-04-28
  • 通讯作者: 张伟,博士,副研究员,硕士生导师,北京大学,深圳北京大学香港科技大学医学中心,广东省深圳市 518036
  • 作者简介:李艳芬,女,1992年生,河南省焦作市人,汉族,在读硕士,主要从事神经损伤与再生研究。
  • 基金资助:

    国家自然科学基金青年科学基金项目(81402600);深圳市科技研发资金知识创新计划基础研究项目(JCYJ20150403110829622)

Interleukin-17A facilitates optic nerve regeneration in a mouse model of optic nerve crush

Li Yan-fen, Ye Xiao-yang, Chen Xiao-fan, Zhang Wei   

  1. Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong Province, China
  • Received:2017-11-16 Online:2018-04-28 Published:2018-04-28
  • Contact: Zhang Wei, Ph.D., Associate researcher, Master’s supervisor, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong Province, China
  • About author:Li Yan-fen, Studying for master’s degree, Shenzhen PKU-HKUST Medical Center, Shenzhen 518036, Guangdong Province, China
  • Supported by:

    the National Natural Science Foundation of China for the Youth, No. 81402600; the Knowledge Innovation and Basic Research Program of Shenzhen Science and Technology Research & Development Foundation, No. JCYJ20150403110829622

摘要:

文章快速阅读:

文题释义:
神经损伤:神经元遭受损失时,会出现尼式小体溶解、细胞核外移、胞体膨大以及突触末端解聚回缩等损伤反应,从而影响生物体正常的生理生化功能。影响损伤神经元再生的因素有多种,对这些影响因素的深入研究是基础神经科学的一个重要课题,也能够为临床上治疗神经损伤提供实验基础和依据。
白细胞介素:白细胞介素是细胞免疫系统中重要的调节因子,参与调节多种免疫相关疾病和癌症的疾病进程,同时在中枢神经系统的神经免疫反应中也发挥着重要作用。
摘要
背景:
实验证明在角膜损伤模型中,白细胞介素17A参与的炎症反应在角膜神经再生过程中发挥着重要作用。
目的:进一步验证白细胞介素17A对视神经损伤模型小鼠神经再生的作用。
方法:构建白细胞介素17A过表达腺病毒载体(AV-IL-17A-GFP),构建白细胞介素17A表达载体,包装并纯化腺病毒,以绿色荧光蛋白空载体包装的腺病毒为对照组(AV-GFP对照组);用病毒感染PC12细胞并通过ELISA检测白细胞介素17A表达效率,采用显微注射方法将病毒注射到小鼠玻璃体内,利用免疫荧光检测视神经中白细胞介素17A的表达,构建小鼠视神经损伤模型,检测受损视神经再生情况。
结果与结论:①与AV-GFP对照组相比,ELISA检测发现AV-IL-17A-GFP组中白细胞介素17A在PC12细胞中高度表达;②免疫荧光染色表明白细胞介素17A在小鼠视神经节细胞和视神经中有效表达,AV-IL-17A-GFP组中再生的视神经纤维数量显著多于对照组,且长度显著长于对照组(P < 0.05);③结果说明,白细胞介素17A腺病毒载体可在小鼠视神经节细胞和视神经中表达,并且能够促进受损视神经的再生。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0003-2170-8852(张伟)

关键词: 白细胞介素17A, 组织构建, 实验动物模型, 小鼠视神经损伤模型, 中枢神经系统, 细胞因子, 腺病毒载体, ELISA, 免疫荧光, 视神经损伤, 神经再生

Abstract:

BACKGROUND: Interleukin-17A has been shown to play an important role in nerve regeneration following corneal injury.
OBJECTIVE: To investigate the effect of interleukin-17A on mouse nerve regeneration after optic nerve injury.
METHODS: The interleukin-17A-overexpressed adenovirus vector (AV-IL-17A-GFP) was constructed and purified. Adenoviruses with green fluorescent protein (AV-GFP) served as control group. PC12 cell lines were transfected with adenoviruses and the expression efficiency of IL-17A were detected by ELISA. The adenovirus was intravitreally injected into the mouse vitreous body, and the expression level of IL-17A in the optic nerve was determined by immunofluorescence. Afterwards, a mouse model of optic nerve injury was established to observe the nerve regeneration. 
RESULTS AND CONCLUSION: ELISA results showed that IL-17A was highly expressed in the PC12 cell lines transfected with AV-IL-17A-GFP compared with the control group. Immunofluorescence results revealed that IL-17A was expressed in vivo successfully. There was a significant increase in the number of regenerated axons in the AV-IL-17A-GFP group compared with the control group (P < 0.05). To conclude, AV-IL-17A-GFP can be expressed in the mature neurons in vivo and promote the axon regeneration after optic nerve crush injury in mice.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Models, Animal, Interleukin-17, Adenoviridae, Nerve Regeneration, Tissue Engineering

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