中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (1): 7-12.doi: 10.3969/j.issn.2095-4344.0403

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架复合骨髓间充质干细胞修复关节骨软骨缺损

鉴晓东，张永红，张 宇，刘来有   

  1. 山西医科大学第二医院骨科，山西省太原市 030001
  • 修回日期:2017-09-02 出版日期:2018-01-08 发布日期:2018-01-08
  • 通讯作者: 张永红，博士，博士生导师，主任医师，山西医科大学第二医院骨科，山西省太原市 030001
  • 作者简介:鉴晓东，男，1991年生，山东省济宁市人，汉族，山西医科大学第二医院骨科在读硕士，主要从事骨与软骨组织工程的研究。
  • 基金资助:

    山西省回国留学人员科研资助项目(2011-109)：新型多聚羟基烷酸软骨组织工程载体的研究

Effect of poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite osteochondral scaffold combined with bone marrow mesenchymal stem cells in the repair of articular osteochondral defects

Jian Xiao-dong, Zhang Yong-hong, Zhang Yu, Liu Lai-you   

  1. Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Revised:2017-09-02 Online:2018-01-08 Published:2018-01-08
  • Contact: Zhang Yong-hong, M.D., Doctoral supervisor, Chief physician, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • About author:Jian Xiao-dong, Studying for master’s degree, Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Supported by:

    the Scientific Research Foundation for the Returned Overseas Chinese Scholars of Shanxi Province, No. 2011-109

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摘要：

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文题释义：
羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架：拟仿照关节结构与功能，以羟基丁酸-羟基辛酸共聚体为主体材料，混合一定比例的Ⅰ型胶原蛋白，采用溶剂浇铸/颗粒沥滤法制备出具有3层结构的羟基丁酸-羟基辛酸共聚体/胶原骨软骨一体化支架，其良好的生物相容性、可降解性、降解无毒性及具有一定的机械强度等特点，为关节软骨缺损区细胞生长提供良好的空间构架，有利于缺损处软骨细胞的增殖及细胞外基质的分泌，适宜的孔径、孔隙率有利于各层之间物质的交换。

 

摘要
背景：前期实验证实，羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架具备适宜的孔隙结构和良好的生物亲和性。
目的：研究羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架复合骨髓间充质干细胞对兔关节负重区骨软骨缺损修复的效果。
方法：取30只成年健康新西兰大白兔，建立右侧膝关节骨软骨缺损模型，随机分3组干预，实验组软骨缺损处植入羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架与骨髓间充质干细胞复合物，对照组软骨缺损处植入羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架，空白对照组不植入任何材料。植入后4，12周，进行骨软骨缺损处Micro-CT扫描、组织学及Ⅱ型胶原免疫组织化学染色观察。
结果与结论：①Micro-CT扫描：至植入后12周时，空白对照组骨软骨缺损未修复；对照组骨软骨缺损缺损修复明显，再生骨小梁结构较多；实验组骨软骨缺损完全修复；②组织学观察：植入4周时，空白对照组缺损区被无定形的组织填充，对照组软骨下骨区域开始出现骨组织的重建，实验组软骨下骨区域出现较多的骨组织重建。植入12周时，空白对照组缺损区无骨小梁结构出现，无软骨陷窝结构；对照组软骨下骨的重建不充分，软骨层的修复由纤维组织构成；实验组骨软骨缺损区修复完全，潮线出现，软骨层厚度与周围正常软骨相似，与周围正常组织完全融合；③Ⅱ型胶原免疫组织化学染色：植入后12周时，空白对照组Ⅱ型胶原基本上不表达，对照组Ⅱ型胶原表达较弱，实验组软骨基质Ⅱ型胶原表达多，与周围正常软骨相似，可清楚看到潮线；④结果表明：羟基丁酸-羟基辛酸共聚体/胶原一体化骨软骨支架复合骨髓间充质干细胞可促进关节负重区骨软骨缺损的修复。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-3750-9065(张永红)

关键词: 羟基丁酸-羟基辛酸共聚体, 骨髓间充质干细胞, 骨软骨缺损, 一体化骨软骨支架, 组织工程, 干细胞

Abstract:

BACKGROUND: Preliminary experiments have confirmed that poly(hydroxybutyrate-co-hydroxyoctanoate) (PHBHOx)/collagen composite osteochondral scaffold exhibits desirable pore structure and biocompatibility.
OBJECTIVE: To investigate the effect of PHBHOx/collagen composite osteochondral scaffold carrying bone marrow mesenchymal stem cells in the repair of articular osteochondral defects in the weight-bearing area of rabbits.
METHODS: Cone-shaped osteochondral defects were created in the femoral medial condyle of the right knee of 30 New Zealand white rabbits. Then, the rabbit models were randomized into three groups and underwent implantation of PHBHOx/collagen composite osteochondral scaffold carrying bone marrow mesenchymal stem cells in the scaffold-cell group, PHBHOx/collagen composite osteochondral scaffold in the scaffold group and no intervention in the control group. At 4 and 12 weeks after surgery, animals were sacrificed for gross, Micro-CT, histological and immunohistochemical collagen II observations.
RESULTS AND CONCLUSION: At 12 weeks after surgery, Micro-CT scanning results suggested that osteochondral defects were not repaired in the control group, repaired incompletely in the scaffold group with many new bone trabeculae, and repaired completely in the scaffold-cell group. Histological results showed that at 4 weeks after surgery, the defects in the control group were filled with amorphous tissues, subchondral bone formation just occurred in the scaffold group but increased in the scaffold-cell group. At 12 weeks after surgery, trabecular bone structure with no cartilage lacuna was observed in the control group; incomplete subchondral bone formation was observed in the scaffold group, and the cartilage layer was repaired by the fibrous tissues; in the scaffold-cell group, osteochondral defect repair was complete, with the emergence of tidal line, and the newborn cartilage was completely integrated with the surrounding normal tissue in addition to a similar thickness. At 12 weeks after surgery, collagen II basically did not express in the control group, weakly expressed in the scaffold group and highly expressed in the scaffold-cell group. In short, the PHBHOx/collagen composite osteochondral scaffold promotes the repair of articular osteochondral defects in the weight-bearing area. 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Cartilage, Tissue Engineering

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