中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (16): 2537-2541.doi: 10.3969/j.issn.2095-4344.0220

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

运动训练对背根神经节切除模型大鼠运动功能的影响

安普天1,朱文文1,李脉超1,崔晓娟1,周  燕1,张益萌1,金利新2   

  1. 青岛大学医学部,1临床医学院,2解剖学教研室,山东省青岛市   266071
  • 收稿日期:2018-01-20 出版日期:2018-06-08 发布日期:2018-06-08
  • 通讯作者: 金利新,硕士,副教授,青岛大学医学部解剖学教研室,山东省青岛市 266071
  • 作者简介:安普天,男,1995年生,青岛大学医学部临床医学院在读本科,主要从事专业临床医学的研究。
  • 基金资助:

    青岛大学医学院国家级实验教学示范中心创新实验项目(201511065055)

Effect of treadmill training on the locomotor function in a rat model of dorsal root ganglion resection

An Pu-tian1, Zhu Wen-wen1, Li Mai-chao1, Cui Xiao-juan1, Zhou Yan1, Zhang Yi-meng1, Jin Li-xin2   

  1. 1School of Clinical Medicine, 2Department of Anatomy, Medical College of Qingdao University, Qingdao 266071, Shandong Province, China
  • Received:2018-01-20 Online:2018-06-08 Published:2018-06-08
  • Contact: Jin Li-xin, Master, Associate professor, Department of Anatomy, Medical College of Qingdao University, Qingdao 266071, Shandong Province, China
  • About author:An Pu-tian, School of Clinical Medicine, Medical College of Qingdao University, Qingdao 266071, Shandong Province, China
  • Supported by:

    the Innovative Experiment Project of National Experimental Teaching Demonstration Center of Medical College of Qingdao University, No. 201511065055

摘要:

文章快速阅读:

文题释义:
周围神经损伤:中枢神经(脑和脊髓)以外的神经由创伤、火器伤、缺血性损伤等原因引起受该神经支配的区域出现感觉障碍、运动障碍和营养障碍,常见临床症状有患肢下垂、肌肉萎缩、感觉丧失、关节挛缩畸形等。部分感觉缺失是周围神经损伤的一种。
囊泡谷氨酸转运蛋白:是谷氨酸能轴突终末的标记物,特异性地分布于几乎所有中枢神经系统的谷氨酸能轴突终末内,对传递和调控外周各种不同的信息刺激发挥重要作用。囊泡谷氨酸转运蛋白1是衡量信息传导通路完整性的重要指标,测定脊髓内囊泡谷氨酸转运蛋白1的表达及分布,可了解神经元终末信息传递情况。
摘要
背景:
周围神经损伤后可导致中枢神经系统通路发生广泛的变化,运动训练对于中枢神经系统损伤后运动功能恢复有一定的促进作用。
目的:观察运动训练后部分感觉缺失模型大鼠的运动功能变化及相应脊髓节段囊泡谷氨酸转运蛋白表达的变化规律,探究运动训练对背根神经节切除模型大鼠运动功能的影响。
方法:10周龄雄性Wistar大鼠39只随机分成实验组(15只)、对照组(15只)和假手术组(9只)。实验组和对照组切除右侧L3、L4背根神经节制备局部感觉缺失的周围神经损伤模型,假手术组只咬除相应关节突,分离至观察到背根神经节。实验组于造模后7 d开始进行15 m/min的跑步机训练,对照组和假手术组自由活动。在术前3 d及术后7,14,21,28 d进行大鼠步态分析,观察模型大鼠行为学变化。于术后7,14,         28 d取大鼠L3脊髓节段制作组织切片,免疫组织化学观察脊髓内囊泡谷氨酸转运蛋白1变化及其特点。
结果与结论:①步态分析:实验组、对照组造模后各时间点腓神经功能指数值均低于术前(P < 0.05)和假手术组(P < 0.05);实验组、对照组造模后7 d腓神经功能指数值最低(P < 0.05),随后逐渐提高,但仍低于术前水平(P < 0.05);实验组造模后21及28 d腓神经功能指数值显著大于对照组(P < 0.05);②脊髓内囊泡谷氨酸转运蛋白1表达:实验组、对照组造模后各时间点脊髓第IX板层囊泡谷氨酸转运蛋白1均明显低于假手术组(P < 0.01);实验组造模后14,28 d脊髓第IX板层囊泡谷氨酸转运蛋白1高于对照组(P < 0.05);实验组与对照组术后各时间点脊髓第IX板层囊泡谷氨酸转运蛋白1表达呈下降趋势(P < 0.05),对照组下降更加明显;③结果表明:运动训练可以促进周围神经损伤后运动功能的恢复。

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程
ORCID: 0000-0001-9369-6817(安普天)

关键词: 脊神经后根神经节切除术, 运动训练, 步态分析, 囊泡谷氨酸转运蛋白, 周围神经损伤, 运动康复, wistar大鼠, 腓神经功能指数

Abstract:

BACKGROUND: Peripheral nerve injury can lead to extensive changes in central nervous system, and exercise training can promote the recovery of locomotor function following central nervous system injury.
OBJECTIVE: To observe the changes of locomotor function and the expression levels of vesicular glutamate transporter VGLUT1 in the spinal cord in a rat model of dorsal root ganglion resection after treadmill exercise and to explore the effect of treadmill training on the locomotor function after peripheral nerve injury.
METHODS: Thirty-nine 10-week-old male Wistar rats were randomized into experimental (n=15), control (n=15) and sham operation (n=9) groups. The rats in the experimental and control groups received the dorsal root ganglion resection at L3 and L4 segments to establish the model of peripheral nerve injury under local anesthesia, while the rats in the sham operation group were only subjected to dorsal root ganglion exposure. The rats in the experimental group underwent 15 m/minute treadmill training at postoperative 7 days, while rats in the other two groups were in free movement. Gait analysis was performed at preoperative 3 days, postoperative 7, 14, 21, and 28 days, respectively, and the behavioral changes of rats were observed. The tissue sections were obtained from L3 segment at postoperative 7, 14, and 28 days to detect the expression levels of VGLUT1 in the spinal cord by immunohistochemistry.
RESULTS AND CONCLUSION: The peroneal nerve function index in the experimental and control groups was lower than that before surgery and that in the sham operation group at postoperative different time points (all P < 0.05). The index in the experimental and control groups was the lowest on day 7 postoperatively (P < 0.05), then the index gradually increased, but was still lower than the preoperative level (P < 0.05). The index in the experimental group was significantly higher than that in the control group at postoperative 21 and 28 days (P < 0.05). The expression levels of VGLUT1 in the lamina IX in the experimental and control groups were significantly lower than those in the sham operation group at different time points after surgery (P < 0.01). The levels in the experimental group were significantly higher than those in the control group at postoperative 14 and 28 days (P < 0.05). The levels in both groups on a decline after surgery, especially the control group (P < 0.05). These results suggest that treadmill can promote the recovery of locomotor function post peripheral nerve injury.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Vesicular Glutamate Transport Proteins, Peripheral Nerves, Rehabilitation

中图分类号: