中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (24): 4422-4426.doi: 10.3969/j.issn.1673-8225.2012.24.011

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

增生性瘢痕成纤维细胞中CyclinD1，CDK2，CDK4作用及与 细胞周期的相关性

金文虎1，王达利1，聂开瑜1，唐红梅2，魏在荣1   

  1. 1遵义医学院附属医院烧伤整形外科，贵州省遵义市 563003；
    2遵义医学院附属美容医院，贵州省遵义市 563003
  • 收稿日期:2011-10-27 修回日期:2011-11-29 出版日期:2012-06-10 发布日期:2013-11-05
  • 通讯作者: 王达利，主任医师，教授，硕士生导师，遵义医学院附属医院烧伤整形外科，贵州省遵义市 563003 daliwangzy@sina.com
  • 作者简介:Jin Wen-hu, Attending physician, Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China jinwenhu1982@163.com
  • 基金资助:

    贵州省社发攻关科研项目(黔科合S字(2007)1040)，课题名称：细胞周期相关基因在增生性瘢痕演变中的作用

Role of CyclinD1, CDK2 and CDK4 in hypertrophic scar fibroblasts and its relativity with cell cycle

Jin Wen-hu1, Wang Da-li1, Nie Kai-yu1, Tang Hong-mei2, Wei Zai-rong1   

  1. 1Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China;
    2Affiliated Aesthetic and Plastic Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Received:2011-10-27 Revised:2011-11-29 Online:2012-06-10 Published:2013-11-05
  • Contact: Wang Da-li, Chief physician, Professor, Master’s supervisor, Department of Burns and Plastic Surgery, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China; daliwangzy@sina.com
  • About author:金文虎，男，1982年生，黑龙江省密山市人，朝鲜族，主治医师，主要从事创伤修复方面研究。 jinwenhu1982@163.com

PDF

480

摘要：

背景：增生性瘢痕的发生和发展可能与细胞周期调节相关基因的异常表达有关。
目的：检测增生性瘢痕不同阶段细胞CyclinD1、CDK2和CDK4 mRNA及蛋白的表达，以及同一标本增生性瘢痕成纤维细胞的细胞周期运行中3者之间的一致性。
方法：采集32份增生性瘢痕标本，以增生性瘢痕形成时段分为3个月组、6个月组、1年组、2年组，各8份，并以8份正常真皮标本作为对照。利用实时荧光定量PCR法、Western blotting法检测标本中CyclinD1、CDK2、CDK4 mRNA及蛋白表达，流式细胞术检测成纤维细胞的细胞周期。
结果与结论：增生性瘢痕标本发展过程中CyclinD1、CDK2、CDK4 mRNA及蛋白表达水平由强至弱变化。3，6个月增生性瘢痕中成纤维细胞主要分布在S、G2 /M期；1，2年增生性瘢痕与对照组成纤维细胞多处于G0/G1期。表明增生性瘢痕不同时期CyclinD1、CDK2、CDK4各自的 mRNA和蛋白表达趋势基本一致，同时增生性瘢痕中成纤维细胞的细胞周期分布与这3个蛋白所执行的功能情况相对应。

关键词: 增生性瘢痕, CyclinD1, CDK2, CDK4, 细胞周期, 成纤维细胞

Abstract:

BACKGROUND: The occurrence and development of hypertrophic scar may be related with the abnormal expression of cell cycle related genes.
OBJECTIVE: To detect the mRNA and protein expression of CyclinD1, CDK2 and CDK4 in fibroblasts at different stages of hypertrophic scar; to explore the consistency of CyclinD1, CDK2 and CDK4 in fibroblasts of the same hypertrophic scar during the cell cycle.
METHODS: A total of 32 hypertrophic scar samples were collected and divided into four groups based on their formation stages of hypertrophic scar: 3 months group, 6 months group, 1 year group and 2 years group, 8 samples for each group; eight normal skin samples were used as control. The mRNA and protein expression of CyclinD1, CDK2 and CDK4 were detected using quantitative real-time PCR and wetern blot. The cell cycle of fibroblasts was detected using flow cytometer.
RESULTS AND CONCLUSION: With the development of hypertrophic scar, the mRNA and protein expression level of CyclinD1, CDK2 and CDK4 in hypertrophic scars changed from strong to weak. Most fibroblasts in hypertrophic scars of the 3 months group and 6 months group were found in the S phase and G2/M phase; most fibroblasts in hypertrophic scars of the 1 year group and 2 years group were found in the G0/G1 phase. These findings indicate that the mRNA and protein expression tendencies of CyclinD1, CDK2 and CDK4 are basically consistent at different stages of hypertrophic scar; meanwhile, the distribution of cell cycle in fibroblasts of hypertrophic scars corresponds with the functions of CyclinD1, CDK2 and CDK4.

中图分类号: