诱导调控牙本质-牙髓再生的牙髓成纤维细胞

doi:10.12307/2021.154

中国组织工程研究 ›› 2021, Vol. 25 ›› Issue (31): 5079-5084.doi: 10.12307/2021.154

• 干细胞综述 stem cell review • 上一篇

诱导调控牙本质-牙髓再生的牙髓成纤维细胞

韦小玲，胡伟平，刘明月，史欣，刘杰，高丽，王思然，段亚君

哈尔滨医科大学附属第二医院口腔修复科，黑龙江省哈尔滨市 150086

收稿日期:2020-07-20 修回日期:2020-07-22 接受日期:2020-09-01 出版日期:2021-11-08 发布日期:2021-04-25
通讯作者: 胡伟平，硕士，主任医师，哈尔滨医科大学附属第二医院口腔修复科，黑龙江省哈尔滨市 150086
作者简介:韦小玲，女，1994年生，海南省海口市人，汉族，2018年哈尔滨医科大学毕业，主要从事口腔修复方面的研究。
基金资助:
黑龙江省医学科学院基金(201801)，项目负责人：胡伟平

Dental pulp fibroblasts inducing and regulating dentin-pulp regeneration

Wei Xiaoling, Hu Weiping, Liu Mingyue, Shi Xin, Liu Jie, Gao Li, Wang Siran, Duan Yajun

Department of Prosthodontics, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China

Received:2020-07-20 Revised:2020-07-22 Accepted:2020-09-01 Online:2021-11-08 Published:2021-04-25
Contact: Hu Weiping, Master, Chief physician, Department of Prosthodontics, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
About author:Wei Xiaoling, Department of Prosthodontics, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
Supported by:
the Academy of Medical Sciences Foundation of Heilongjiang Province, No. 201801 (to HWP)

摘要/Abstract

摘要：

文题释义：

牙髓成纤维细胞：是一类特别的细胞群，它们是唯一的非肝脏、非免疫来源但却能合成补体蛋白的细胞种类，这类细胞可以使补体蛋白产生具有生物活性的片段，如C3a、C5a和膜攻击复合物，在牙髓再生过程中起到重要的作用。
牙本质-牙髓再生：牙髓组织位于闭锁的牙本质壁内，牙髓腔内循环极差，当受到外界刺激时牙髓易产生水肿，引发炎症，从而导致牙髓坏死。牙本质-牙髓再生是指牙髓受到外界刺激时可分泌相关蛋白产生局部调节作用，有一定的修复再生功能。
背景：在牙髓-牙本质再生过程中，牙髓内其他类型的细胞如成牙本质细胞和干细胞一直广受学者关注和研究，对于成纤维细胞的研究甚少，对牙髓成纤维细胞的研究可为今后牙髓治疗提供新的细胞来源。
目的：综述牙髓成纤维细胞在牙髓-牙本质再生过程中的作用。
方法：应用计算机在PubMed数据库、中国知网数据库，检索2000至2020年间关于牙髓成纤维细胞和牙髓-牙本质再生的相关文献，英文检索词为“pulp、fibroblast、dentin-pulp、regeneration、complement、C5a、C3a”，中文检索词为“牙髓、成纤维细胞、牙本质-牙髓、补体、C3a、C5a”，最终对56篇文献进行归纳总结。
结果与结论：成纤维细胞是牙髓中数量最多的一种细胞，该细胞在正常及病理状态下对牙髓免疫反应及功能起着重要的调节作用。它们可以使补体蛋白产生具有生物活性的片段，如C3a、C5a和膜攻击复合物，同时通过分泌生长因子对修复性牙本质的合成、血管再生和神经的生长分布进行精细调控，这在牙髓-牙本质再生过程中发挥着重要作用。因此，除了成牙本质细胞和牙髓干细胞外，牙髓成纤维细胞也应该被视为诱导牙本质-牙髓再生策略中的一种重要细胞。
https://orcid.org/0000-0002-3441-4565(韦小玲)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 成纤维细胞, 牙髓, 牙本质, 补体, 生长因子, 牙髓再生, C5a, C3a, MAC, 综述

Abstract:

BACKGROUND: During pulp-dentin regeneration, other types of cells in dental pulp, such as dentin cells and stem cells, have been widely concerned and studied by scholars, while fibroblasts have been rarely studied. The study on dental pulp fibroblasts may provide a new source of cells for future pulp treatment.
OBJECTIVE: To review the role of dental pulp fibroblasts in pulp-dentin regeneration.
METHODS: The computer was applied to search articles on dental pulp fibroblasts and dental pulp dentine regeneration published from 2000 to 2020 inPubMed and CNKI databases. The English search terms were “pulp, fibroblast, dentin-pulp, regeneration, complement, C5a, C3a”. The Chinese search terms were “dental pulp, fibroblasts, dentin-pulp, complement, C3a, C5a”. Finally, 56 articles were summarized.
RESULTS AND CONCLUSION: Fibroblasts are the most abundant cells in dental pulp. Fibroblasts play an important role in regulating the immune response and function of dental pulp under normal and pathological conditions. They enable complement proteins to produce bioactive fragments, such as C3a, C5a and membrane-attack complexes, and simultaneously, through the secretion of growth factors, fine-regulate the synthesis of restorative dentin, angiogenesis and the distribution of nerve growth, which play an important role in the pulp and dentin regeneration. Therefore, in addition to odontoblasts and pulp stem cells, dental pulp fibroblasts should also be considered as an important cell in the strategy of inducing dentin-pulp regeneration.

Key words: fibroblast, dental pulp, dentin, complement, growth factor, regeneration of dental pulp, C5a, C3a, MAC, review

中图分类号:

韦小玲, 胡伟平, 刘明月, 史欣, 刘杰, 高丽, 王思然, 段亚君 . 诱导调控牙本质-牙髓再生的牙髓成纤维细胞[J]. 中国组织工程研究, 2021, 25(31): 5079-5084.

Wei Xiaoling, Hu Weiping, Liu Mingyue, Shi Xin, Liu Jie, Gao Li, Wang Siran, Duan Yajun. Dental pulp fibroblasts inducing and regulating dentin-pulp regeneration[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 5079-5084.

图/表（结果） 1

参考文献

[1] ABOUT I. “The stem cell fashion”: do we need only stem cells for tissue regeneration? Clin Oral Investig. 2018;22(2):553-554.
[2] ABOUT I, BOTTERO MJ, DE DENATO P, et al. Human dentin production in vitro. Exp Cell Res. 2000;258(1):33-41.
[3] IOHARA K, ZHENG L, WAKE H, et al. A novel stem cell source for vasculogenesis in ischemia: subfraction of side population cells from dental pulp. Stem Cells. 2008;26(9):2408-2418.
[4] BAKOPOULOU A, ABOUT I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016;2016:4209891.
[5] BAKOPOULOU A, HUANG GT, ROUABHIA M, et al. Advances and New Technologies towards Clinical Application of Oral Stem Cells and Their Secretome. Stem Cells Int. 2017;2017:6367375.
[6] CHMILEWSKY F, ABOUT I, CHUNG SH. Pulp Fibroblasts Control Nerve Regeneration through Complement Activation. J Dent Res. 2016;95(8):913-922.
[7] BAKHTIAR H, AMINISHAKIB P, ELLINI MR, et al. Dental Pulp Response to RetroMTA after Partial Pulpotomy in Permanent Human Teeth. J Endod. 2018;44(11):1692-1696.
[8] BRAGA RR, ABOUT I. How far do calcium release measurements properly reflect its multiple roles in dental tissue mineralization? Clin Oral Investig. 2019;23(1):501.
[9] GIRAUD T, JEANNEAU C, ROMBOUTS C, et al. Pulp capping materials modulate the balance between inflammation and regeneration. Dent Mater. 2019;35(1):24-35.
[10] LIN LM, HUANG GT. Regeneration and Repair in Endodontics-A Special Issue of the Dentistry Journal. Dent J (Basel). 2015;3(3):77-78.
[11] JEANNEAU C, LUNDY FT, EL KARIM IA, et al. Potential Therapeutic Strategy of Targeting Pulp Fibroblasts in Dentin-Pulp Regeneration. J Endod. 2017;43(9S):S17-S24.
[12] CHMILEWSKY F, JEANNEAU C, LAURENT P, et al. Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration. Am J Pathol. 2014;184(7):1991-2000.
[13] WILSON DG, PHAMLUONG K, LI L, et al. Global defects in collagen secretion in a Mia3/TANGO1 knockout mouse. J Cell Biol. 2011;193(5):935-951.
[14] XOURI G, CHRISTIAN S. Origin and function of tumor stroma fibroblasts. Semin Cell Dev Biol. 2010;21(1):40-46.
[15] TÉCLÈS O, LAURENT P, ZYGOURITSAS S, et al. Activation of human dental pulp progenitor/stem cells in response to odontoblast injury. Arch Oral Biol. 2005; 50(2):103-108.
[16] CHMILEWSKY F, ABOUT I, CHUNG SH. C5L2 Receptor Represses Brain-Derived Neurotrophic Factor Secretion in Lipoteichoic Acid-Stimulated Pulp Fibroblasts. J Dent Res. 2017;96(1):92-99.
[17] CHMILEWSKY F, ABOUT I, COOPER LF, et al. C5L2 Silencing in Human Pulp Fibroblasts Enhances Nerve Outgrowth Under Lipoteichoic Acid Stimulation. J Endod. 2018;44(9):1396-1401.
[18] LE FOURNIS C, HADJICHRISTOU C, JEANNEAU C, et al. Human Pulp Fibroblast Implication in Phagocytosis via Complement Activation. J Endod. 2019;45(5):584-590.
[19] ARTESE L, RUBINI C, FERRERO G, et al. Vascular endothelial growth factor (VEGF) expression in healthy and inflamed human dental pulps. J Endod. 2002;28(1):20-23.
[20] COHEN S. Dentinal pulpal reaction to dental caries. Alpha Omegan. 1967;60(2): 119-125.
[21] TRAN-HUNG L, LAURENT P, CAMPS J, et al. Quantification of angiogenic growth factors released by human dental cells after injury. Arch Oral Biol. 2008;53(1):9-13.
[22] EL KARIM IA, LINDEN GJ, IRWIN CR, et al. Neuropeptides regulate expression of angiogenic growth factors in human dental pulp fibroblasts. J Endod. 2009;35(6): 829-833.
[23] KILLOUGH SA, LUNDY FT, IRWIN CR. Substance P expression by human dental pulp fibroblasts: a potential role in neurogenic inflammation. J Endod. 2009;35(1):73-77.
[24] NOSRAT IV, SMITH CA, MULLALLY P, et al. Dental pulp cells provide neurotrophic support for dopaminergic neurons and differentiate into neurons in vitro; implications for tissue engineering and repair in the nervous system. Eur J Neurosci. 2004;19(9):2388-2398.
[25] [YANG JW, ZHANG YF, SUN ZY, et al. Dental pulp tissue engineering with bFGF-incorporated silk fibroin scaffolds. J Biomater Appl. 2015;30(2):221-229.
[26] NOVAIS A, LESIEUR J, SADOINE J, et al. Priming Dental Pulp Stem Cells from Human Exfoliated Deciduous Teeth with Fibroblast Growth Factor-2 Enhances Mineralization Within Tissue-Engineered Constructs Implanted in Craniofacial Bone Defects. Stem Cells Transl Med. 2019;8(8):844-857.
[27] JEANNEAU C, GIRAUD T, LAURENT P, et al. BioRoot RCS Extracts Modulate the Early Mechanisms of Periodontal Inflammation and Regeneration. J Endod. 2019;45(8):1016-1023.
[28] KAJIHARA I, JINNIN M, HONDA N, et al. Scleroderma dermal fibroblasts overexpress vascular endothelial growth factor due to autocrine transforming growth factor β signaling. Mod Rheumatol. 2013;23(3):516-524.
[29] TRAN-HUNG L, MATHIEU S, ABOUT I. Role of human pulp fibroblasts in angiogenesis. J Dent Res. 2006;85(9):819-823.
[30] LILLESAAR C, ERIKSSON C, JOHANSSON CS, et al. Tooth pulp tissue promotes neurite outgrowth from rat trigeminal ganglia in vitro. J Neurocytol. 1999;28(8): 663-670.
[31] JEANNEAU C, GIRAUD T, MILAN JL, et al. Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation. Clin Oral Investig. 2020;24(2):639-647.
[32] SMITH AJ, TOBIAS RS, CASSIDY N, et al. Odontoblast stimulation in ferrets by dentine matrix components. Arch Oral Biol. 1994;39(1):13-22.
[33] HOWARD C, MURRAY PE, NAMEROW KN. Dental pulp stem cell migration. J Endod. 2010;36(12):1963-1966.
[34] MATHIEU S, JEANNEAU C, SHEIBAT-OTHMAN N, et al. Usefulness of controlled release of growth factors in investigating the early events of dentin-pulp regeneration. J Endod. 2013;39(2):228-235.
[35] SAKAI VT, ZHANG Z, DONG Z, et al. SHED differentiate into functional odontoblasts and endothelium. J Dent Res. 2010;89(8):791-796.
[36] GIRAUD T, RUFAS P, CHMILEWSKY F, et al. Complement Activation by Pulp Capping Materials Plays a Significant Role in Both Inflammatory and Pulp Stem Cells’ Recruitment. J Endod. 2017;43(7):1104-1110.
[37] RICKLIN D, HAJISHENGALLIS G, YANG K, et al. Complement: a key system for immune surveillance and homeostasis. Nat Immunol. 2010;11(9):785-797.
[38] HAENEY MR. The role of the complement cascade in sepsis. J Antimicrob Chemother. 1998;41 Suppl A:41-46.
[39] TOMLINSON S. Complement defense mechanisms. Curr Opin Immunol. 1993; 5(1):83-89.
[40] HARTMANN K, HENZ BM, KRÜGER-KRASAGAKES S, et al. C3a and C5a stimulate chemotaxis of human mast cells. Blood. 1997;89(8):2863-2870.
[41] TENGVALL P, ASKENDAL A, LUNDSTRÖM II. Ellipsometric in vitro studies on the activation of complement by human immunoglobulins M and G after adsorption to methylated silicon. Colloids Surf B Biointerfaces. 2001;20(1):51-62.
[42] ANDERSSON J, EKDAHL KN, LARSSON R, et al. C3 adsorbed to a polymer surface can form an initiating alternative pathway convertase. J Immunol. 2002;168(11): 5786-5791.
[43] CHMILEWSKY F, LIANG R, KANAZAWA M, et al. C5L2 Regulates DMP1 Expression during Odontoblastic Differentiation. J Dent Res. 2019;98(5):597-604.
[44] GRONTHOS S, BRAHIM J, LI W, et al. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002;81(8):531-535.
[45] BATOULI S, MIURA M, BRAHIM J, et al. Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res. 2003;82(12):976-981.
[46] SCHRAUFSTATTER IU, DISCIPIO RG, ZHAO M, et al. C3a and C5a are chemotactic factors for human mesenchymal stem cells, which cause prolonged ERK1/2 phosphorylation. J Immunol. 2009;182(6):3827-3836.
[47] CHMILEWSKY F, JEANNEAU C, LAURENT P, et al. Pulp progenitor cell recruitment is selectively guided by a C5a gradient. J Dent Res. 2013;92(6):532-539.
[48] CHMILEWSKY F, AYAZ W, APPIAH J, et al. Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth. Sci Rep. 2016;6:31799.
[49] BJÖRK J, HUGLI TE, SMEDEGÅRD G. Microvascular effects of anaphylatoxins C3a and C5a. J Immunol. 1985;134(2):1115-1119.
[50] CARMONA-FONTAINE C, THEVENEAU E, TZEKOU A, et al. Complement fragment C3a controls mutual cell attraction during collective cell migration. Dev Cell. 2011;21(6):1026-1037.
[51] RUFAS P, JEANNEAU C, ROMBOUTS C, et al. Complement C3a Mobilizes Dental Pulp Stem Cells and Specifically Guides Pulp Fibroblast Recruitment. J Endod. 2016;42(9):1377-1384.
[52] PEDANO MS, LI X, JEANNEAU C, et al. Survival of human dental pulp cells after 4-week culture in human tooth model. J Dent. 2019;86:33-40.
[53] GUIVARC’H M, JEANNEAU C, GIRAUD T, et al. An international survey on the use of calcium silicate-based sealers in non-surgical endodontic treatment. Clin Oral Investig. 2020;24(1):417-424.
[54] JEANNEAU C, RUFAS P, ROMBOUTS C, et al. Can Pulp Fibroblasts Kill Cariogenic Bacteria? Role of Complement Activation. J Dent Res. 2015;94(12):1765-1772.
[55] LOESCHE WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev. 1986;50(4):353-380.
[56] ROMBOUTS C, JEANNEAU C, BAKOPOULOU A, et al. Dental Pulp Stem Cell Recruitment Signals within Injured Dental Pulp Tissue. Dent J (Basel). 2016;4(2):8.

引言

牙髓组织位于密闭且坚硬的牙本质壁内，它含有丰富的细胞、血管、神经以及纤维等多种成分，而成纤维细胞是牙髓中数量最多的一种细胞。牙髓内其他类型细胞如成牙本质细胞和干细胞一直广受学者关注和研究，然而对于成纤维细胞的研究甚少[1]。有研究表明，该细胞在正常及病理状态下对牙髓免疫反应及功能起着重要的调节作用，并且牙髓成纤维细胞及其合成的生长因子可促进牙髓-牙本质、牙髓血管的再生和神经分布，见图1。牙髓成纤维细胞还是一类特别的细胞群，这类细胞可以使补体蛋白产生具有生物活性的片段，如C3a、C5a和膜攻击复合物，在牙髓再生过程中起到重要的作用。C3a片段参与诱导牙髓间充质干细胞的增殖并促进其迁移，同时也与牙髓间充质干细胞的活化和牙髓成纤维细胞的募集相关。C5a可介导牙髓内神经的发生及干细胞的募集。膜攻击复合物作用在致龋菌细胞壁上，直接瓦解细菌，从而保护牙髓。这些研究结果表明牙髓成纤维细胞在牙本质-牙髓再生中起到重要的调节作用，可直接破坏致龋菌并释放与神经生长、牙髓干细胞募集和牙髓再生有关的生物活性片段。综上所述，牙髓成纤维细胞可用于研究诱导牙本质-牙髓再生。

牙髓内循环较差，当发生炎症时极易引起牙髓组织水肿、髓腔压力增大从而导致牙髓炎的发生，最终牙髓坏死。然而许多证据表明，牙髓对外界刺激会产生局部的调节反应，具有一定的修复再生功能。牙髓再生是指通过各种手段以促进或恢复牙髓活力，达到治疗牙髓感染或牙髓坏死的目的，这为临床治疗牙髓炎症提供了新的治疗思路。自2002年提出牙髓干细胞以来，大多数学者致力于研究牙髓间充质干细胞分化为成牙本质样细胞在牙髓牙本质再生中的作用，并在其激活及募集信号上进行了大量的研究[2]。越来越多的体外研究证实了这些细胞向其他类型细胞分化的潜能，以及体内实验中分化为诸如骨、软骨和血管等其他组织的再生潜能[3]。相比骨髓间充质干细胞，牙髓干细胞具有更强的增殖、分化和再生能力。如何利用如此“高能”的牙髓干细胞在牙髓炎发生时发挥修复和再生功能，从而利于牙髓恢复健康，值得学者们进一步研究。
牙髓成纤维细胞是牙髓中所占比例最高的一种细胞，近年来，一系列文章指出牙髓内的成纤维细胞具有募集干细胞并分泌补体蛋白的作用，可促进牙髓修复和再生[4-10]。牙髓成纤维细胞作为一种主要信号来源，通过提供激活、引导牙髓再生的信号来启动牙髓再生进程，从而保护牙髓免受致龋菌侵袭，该文章对此进行了阐述，并着重强调了牙髓成纤维细胞在牙髓炎症中的抗炎作用。
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

材料方法

讨论

3.1 既往他人在该领域研究的贡献和存在的问题在牙髓免疫与再生方面仍需要进行机制方面的研究，如免疫细胞通过哪些信号通路激活，以及牙髓内的免疫负调控在牙髓再生过程中的作用仍需要学者们继续探讨研究。
3.2 作者综述区别于他人他篇的特点作者将目前已发表的牙髓免疫与再生方面的文献进行了梳理与归纳总结，并加以讨论，旨在为牙髓免疫再生领域提供新的基础背景介绍及总结。
3.3 综述的局限性该综述的局限性在于作者未对已有文献的结果进行验证，正在开展牙髓免疫与再生方面的研究。
3.4 综述的重要意义该综述论述了牙髓成纤维细胞在炎症进程中对控制细菌增殖起着重要的作用。牙髓成纤维细胞分泌多种生长因子和补体活性片段，通过诱导血管化、神经分布和牙髓-牙本质再生在牙髓组织再生过程中起重要作用，同时也在抗龋中扮演着重要角色。目前，学者们对成牙本质细胞和牙髓干细胞的研究火热，而对牙髓成纤维细胞的研究较少。虽然，牙本质-牙髓再生的研究已取得了许多成绩，但是牙髓炎的发生以及牙髓的修复是一个复杂的过程，对其机制的研究尚待明确，且在实际临床应用中还面临着许多的困难。
综上，除了成牙本质细胞和牙髓干细胞外，牙髓成纤维细胞也应该被视为诱导牙本质-牙髓再生策略中的一种重要细胞。关于牙髓成纤维细胞在牙髓再生中是如何发挥作用，通过什么机制促进牙髓再生，是否能作为新的细胞来源用于其他部位的组织再生，这还有待进一步研究。
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

诱导调控牙本质-牙髓再生的牙髓成纤维细胞

Dental pulp fibroblasts inducing and regulating dentin-pulp regeneration

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表（结果） 1

参考文献

相关文章 15

引言

材料方法

讨论

文章快阅

延伸阅读

编辑推荐

Metrics

本文评价

[1]	沈江涌, 贺茜, 唐玉婷, 王建军, 刘金毅, 陈园园, 王昕艺, 刘彤, 孙浩原. 铁死亡诱导剂RAS合成致死分子3抑制病理性瘢痕成纤维细胞的纤维化[J]. 中国组织工程研究, 2024, 28(8): 1168-1173.
[2]	王雯, 郑芃芃, 孟浩浩, 刘浩, 袁长永. 过表达Sema3A促进牙髓干细胞和MC3T3-E1的成骨分化[J]. 中国组织工程研究, 2024, 28(7): 993-999.
[3]	买斯吐热木·黑力力, 张婉霞, 尼加提·努尔穆罕默德, 买买提吐逊·吐尔地. 关节腔注射医用臭氧对早期颞下颌骨关节炎模型大鼠髁突组织学的影响[J]. 中国组织工程研究, 2024, 28(4): 505-509.
[4]	马思聪 , 陈晶, 李云庆. 结缔组织生长因子在神经系统中的功能与作用[J]. 中国组织工程研究, 2024, 28(4): 615-620.
[5]	亓明, 王磊, 张振. CXCL5通过诱导血管钙化参与颈动脉斑块的形成[J]. 中国组织工程研究, 2024, 28(2): 186-192.
[6]	左俊, 马少林. β-谷甾醇对增生性瘢痕成纤维细胞作用机制的网络药理学分析[J]. 中国组织工程研究, 2024, 28(2): 216-223.
[7]	姚思琦, 黎文正, 汪虹. 转化生长因子β/Smad信号通路与瘢痕疙瘩的靶向治疗[J]. 中国组织工程研究, 2024, 28(16): 2619-2624.
[8]	吴玉珍, 孙青, 刘霞, 周雨, 金其贯. 有氧运动干预2型糖尿病大鼠肾功能的变化[J]. 中国组织工程研究, 2024, 28(14): 2145-2151.
[9]	左俊, 马少林. 增生性瘢痕差异表达基因及小分子药物预测的生物信息学分析与验证[J]. 中国组织工程研究, 2024, 28(14): 2166-2172.
[10]	张红霞, 栗兴超, 高玺鑫, 张晓, 梅双, 马寒夕, 张天. 不同软组织替代物在比格犬尖牙唇侧区域移植后附着龈的厚度和组织学变化[J]. 中国组织工程研究, 2024, 28(11): 1660-1665.
[11]	王钟庆, 熊贤梅, 张严, 李世杰, 马立琼, 卢泽声, 高怡加. 三七总皂苷上调浓缩生长因子释放促进大鼠骨折愈合[J]. 中国组织工程研究, 2024, 28(11): 1678-1683.
[12]	莫坚, 叶森涛, 章晓云. 中药单体及复方在膝骨关节炎中的治疗进展[J]. 中国组织工程研究, 2024, 28(11): 1756-1761.
[13]	卢洁, 金杰, 于俪超, 马莎莎, 许红梅. 红光联合银离子敷料治疗慢性难愈合创面的最佳方案筛选及机制[J]. 中国组织工程研究, 2024, 28(10): 1554-1561.
[14]	饶瑾, 姜水, 石海山. 基于炎症微环境调控组织工程再生性牙髓治疗[J]. 中国组织工程研究, 2024, 28(10): 1620-1625.
[15]	孙菁, 廖健, 孙江龄, 程萍, 冯红超. 重组人生长激素促进人牙髓干细胞的成骨分化[J]. 中国组织工程研究, 2024, 28(1): 56-61.