中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4209-4212.doi: 10.3969/j.issn.1673-8225.2012.23.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

间断应用甲状旁腺激素对人骨髓基质干细胞的影响

殷晓雪，周 方，张志山，宋纯理   

  1. 北京大学第三医院骨科，北京市 100191
  • 收稿日期:2011-10-26 修回日期:2011-12-01 出版日期:2012-06-03 发布日期:2013-11-06
  • 通讯作者: 周方，博士，主任医师，教授，北京大学第三医院骨科，北京市 100191
  • 作者简介:殷晓雪☆， 1972年生，陕西省商南县人，汉族，2003年北京大学医学部毕业，博士，副研究员，主要从事骨科相关疾病的基础研究。 luckyemail2008@sina.com

Effect of intermittent treatment with parathyroid hormone on human bone marrow stromal stem cells

Yin Xiao-xue, Zhou Fang, Zhang Zhi-shan, Song Chun-li   

  1. Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China
  • Received:2011-10-26 Revised:2011-12-01 Online:2012-06-03 Published:2013-11-06
  • Contact: Zhou Fang, M.D., Chief physician, Professor, Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China
  • About author:Yin Xiao-xue☆, M.D., Associate researcher, Department of Orthopedics, Third Hospital of Peking University, Beijing 100083, China luckyemail2008@sina.com

PDF

341

被引次数

6

摘要：

背景：持续应用和间断应用甲状旁腺激素以及使用量的大小对骨的效应有很大差异。
目的：观察不同剂量的甲状旁腺激素间断给药对人骨髓基质干细胞增殖和分化的影响。
方法：体外培养人骨髓基质干细胞，分为空白对照组和甲状旁腺激素5，20及50 nmo/L组，每天作用1 h，MTT 法检测人骨髓基质干细胞的增殖；碱性磷酸酶活性测定检测人骨髓基质干细胞向成骨细胞的分化。
结果与结论：甲状旁腺激素50 nmol/L每天作用1 h可显著促进人骨髓基质干细胞的增殖，同时使碱性磷酸酶升高，均显著高于其他3组。结果证实50 nmo/L甲状旁腺激素间断应用可以促进人骨髓基质干细胞的增殖和向成骨细胞的分化。

关键词: 甲状旁腺激素, 骨髓基质干细胞, 增殖, 分化, 成骨细胞

Abstract:

BACKGROUND: Continuous and intermittent application of parathyroid hormone at different doses can cause different effects on bone.
OBJECTIVE: To observe the effect of intermittent treatment with different doses of parathyroid hormone on the proliferation and differentiation of human bone marrow stromal stem cells.
METHODS: Human bone marrow stromal stem cells were cultured in vitro and divided into blank control group and 5, 20 and 50 nmo/L parathyroid hormone group, and the parathyroid hormone groups were treated for 1 hour per day. MTT and alkaline phosphatase activities were used to detect the proliferation and differentiation of human bone marrow stromal stem cells to osteoblasts.
RESULTS AND CONCLUSION: 50 nmol/L of parathyroid hormone treated for 1 hour per day could greatly promote the proliferation of human bone marrow stromal stem cells and could increase the alkaline phosphatase activities, and the alkaline phosphatase activities in 50 nmo/L parathyroid hormone group were higher than other three groups. Intermittent treatment with 50 nmol/L parathyroid hormone can promote the proliferation and differentiation of human bone marrow stromal stem cells.

中图分类号: