中国组织工程研究 ›› 2022, Vol. 26 ›› Issue (7): 1045-1050.doi: 10.12307/2022.142

• 脐带脐血干细胞 umbilical cord blood stem cells • 上一篇    下一篇

白细胞介素8受体可提高脐带间充质干细胞迁移和向损伤内皮的黏附

朱兵兵1,2,邓江华2,陈晶晶2,慕晓玲2   

  1. 1新疆医科大学基础医学院人体解剖学教研室,新疆维吾尔自治区乌鲁木齐市  830000; 2石河子大学医学院,新疆维吾尔自治区石河子市  832000
  • 收稿日期:2020-10-26 修回日期:2020-10-29 接受日期:2020-11-26 出版日期:2022-03-08 发布日期:2021-10-29
  • 通讯作者: 慕晓玲,博士,教授,石河子大学医学院,新疆维吾尔自治区石河子市 832000
  • 作者简介:朱兵兵,女,1995 年生,新疆维吾尔自治区沙湾县人,汉族,2020年石河子大学毕业,硕士,主要从事干细胞的基础与应用研究。
  • 基金资助:
    新疆兵团社会发展科技攻关与成果转化计划(2016AD013),项目负责人:慕晓玲;自治区研究生教育创新计划科研创新项目(XJGRI201604),项目负责人:慕晓玲

Interleukin-8 receptor enhances the migration and adhesion of umbilical cord mesenchymal stem cells to injured endothelium

Zhu Bingbing1, 2, Deng Jianghua2, Chen Jingjing2, Mu Xiaoling2   

  1. 1Department of Human Anatomy, School of Basic Medicine, Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China; 2School of Medicine, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China
  • Received:2020-10-26 Revised:2020-10-29 Accepted:2020-11-26 Online:2022-03-08 Published:2021-10-29
  • Contact: Mu Xiaoling, MD, Professor, School of Medicine, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China
  • About author:Zhu Bingbing, Master, Department of Human Anatomy, School of Basic Medicine, Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China; School of Medicine, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    the Social Development Science and Technology Research and Achievement Transformation Plan of Xinjiang Production and Construction Corps, No. 2016AD013 (to MXL); the Research and Innovation Project of Postgraduate Education Innovation Plan of the Autonomous Region, No. XJGRI201604 (to MXL)

摘要:

文题释义:
脐带间充质干细胞:具有自我更新和多向分化潜能,免受异体移植排斥反应,通过旁分泌和自分泌作用释放多种生物活性因子,抑制炎症反应,进行免疫调节,促进缺血组织的再生和修复。
白细胞介素8受体:①白细胞介素8受体A可特异性与白细胞介素8结合,为高亲和力受体,主要分布于中性粒细胞、单核细胞、T细胞等;②白细胞介素8受体B除与白细胞介素8结合,还可与MGSA/GRO、NAP-2等趋化因子结合,主要分布于中性粒细胞和某些髓样前体细胞系。
背景:内皮功能障碍是动脉粥样硬化和心血管疾病的早期预测因子,脐带间充质干细胞移植为治疗血管损伤提供了可能性。
目的:探讨过表达白细胞介素8受体A/B对人脐带间充质干细胞迁移能力的影响。
方法培养原代人脐带间充质干细胞和人脐静脉内皮细胞,转染含白细胞介素8受体A/B的腺病毒载体,荧光共聚焦和Western blot检测转染后细胞中白细胞介素8受体A/B的表达,然后通过CCK-8实验、竞争实验和黏附实验检测过表达白细胞介素8受体A/B对细胞增殖能力、竞争能力和迁移能力的影响。
结果与结论:过表达白细胞介素8受体A/B的荧光腺病毒载体对细胞增殖能力无明显影响,但过表达白细胞介素8受体A/B的人脐带间充质干细胞竞争和迁移能力明显高于过表达白细胞介素8受体A/B的人脐静脉内皮细胞(P < 0.05)。结果表明,白细胞介素8受体提高了人脐带间充质干细胞的迁移能力,促进向损伤内皮的黏附。

https://orcid.org/0000-0003-3713-7468(朱兵兵) 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

关键词: 干细胞, 人脐带间充质干细胞, 人脐静脉内皮细胞, 白细胞介素8受体, 腺病毒, 增殖, 竞争, 黏附

Abstract: BACKGROUND: Endothelial dysfunction is an early predictor of atherosclerosis and cardiovascular disease. Umbilical cord mesenchymal stem cell transplantation provides the possibility for the treatment of vascular injury.
OBJECTIVE: To explore the effect of overexpression of interleukin-8 receptor A/B on migration of human umbilical cord mesenchymal stem cells. 
METHODS: Primary cultured human umbilical cord mesenchymal stem cells and human umbilical vein endothelial cells were transfected with adenovirus vector containing interleukin-8 receptor A/B. Confocal immunofluorescence microscopy and western blot assay were used to observe the expression of interleukin-8 receptor A/B in cells. CCK-8 assay, competition test and adhesion test were used to detect the effects of interleukin-8 receptor A/B overexpression on cell proliferation, competitiveness and migration. 
RESULTS AND CONCLUSION: The fluorescent adenovirus vector overexpressing interleukin-8 receptor A/B has no significant effect on cell proliferation. However, the competition and migration abilities of human umbilical cord mesenchymal stem cells overexpressing interleukin-8 receptor A/B were significantly higher than those of human umbilical vein endothelial cells overexpressing interleukin-8 receptor A/B (P < 0.05). It is concluded that interleukin-8 receptor enhances the migration ability of human umbilical cord mesenchymal stem cells and promotes adhesion to injured endothelium.


Key words: stem cells, human umbilical cord mesenchymal stem cells, human umbilical vein endothelial cells, interleukin-8 receptor, adenovirus, proliferation, competition, adhesion

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