中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3907-3910.doi: 10.3969/j.issn.1673-8225.2012.21.025

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

钙磷涂层镁合金植入体周围组织中骨形成蛋白2的表达*****☆

孙  伟1，张广道1，谭丽丽2，杨  柯2，洪岩松1，艾红军1   

  1. 1中国医科大学口腔医学院口腔修复科，辽宁省沈阳市  110002；2中国科学院金属研究所，辽宁省沈阳市  110016
  • 收稿日期:2012-01-15 修回日期:2012-03-15 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 艾红军，教授，博士生导师，中国医科大学口腔医学院口腔修复科，辽宁省沈阳市 110002 aih0620@yahoo.com.cn
  • 作者简介:孙伟☆，男，1977年生，辽宁省抚顺市人，汉族，中国医科大学毕业，博士，讲师，主要从事口腔修复科研、教学和临床工作。sunway_77@ yahoo.com.cn
  • 基金资助:

    辽宁省自然科学基金资助项目(20082079)；辽宁省教育厅资助项目(L2010644，2009A767，2008822)；沈阳市科技计划资助项目(F10-149-9-42)。

Bone morphogenetic protein-2 expression in the surrounding tissues of calcium phosphate coating magnesium alloys

Sun Wei1, Zhang Guang-dao1, Tan Li-li2, Yang Ke2, Hong Yan-song1, Ai Hong-jun1   

  1. 1Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang  110002, Liaoning Province, China; 2Institute of Metals Research, Chinese Academy of Sciences, Shenyang  110016, Liaoning Province, China
  • Received:2012-01-15 Revised:2012-03-15 Online:2012-05-20 Published:2012-05-20
  • Contact: Ai Hong-jun, Professor, Doctoral supervisor, Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China aih0620@yahoo.com.cn
  • About author:Sun Wei☆, Doctor, Lecturer, Department of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, Liaoning Province, China sunway_77@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 20082079*; Education Department Foundation of Liaoning Province, No. L2010644*, 2009A767*, 2008822*; Science and Technology Program Foundation of Shenyang City, No. F10-149-9-42*

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摘要：

背景：改变合金构成可以改善镁合金的耐腐蚀性和机械性能。
目的：观察钙磷涂层对镁合金植入体周围软组织中骨形成蛋白2表达的影响。
方法：将27只兔随机分成3组，分别在兔下颌骨内植入钙磷涂层镁合金螺钉、镁合金螺钉及钛合金，植入后1，2，3个月，采用反转录聚合酶链技术检测植入体周围软组织中骨形成蛋白2 mRNA的表达情况。
结果与结论：钙磷涂层镁合金螺钉组、镁合金螺钉组周围软组织中骨形成蛋白2 mRNA的表达，随时间的增加逐渐增高，钙磷涂层镁合金螺钉组内各时间点间骨形成蛋白2 mRNA的表达水平差异有显著性意义(P < 0.05)，且镁合金螺钉组植入后1，2个月周围软组织中骨形成蛋白2 mRNA的表达水平高于钙磷涂层镁合金螺钉组(P < 0.05)。显示钙磷涂层能够促进骨形成蛋白2 mRNA的表达，并抑制其过度表达，有利于早期成骨过程，表现出良好的生物活性。
 

关键词: 镁合金, 涂层, 骨形成蛋白2, 磷酸三钙, 螺钉

Abstract:

BACKGROUND: The alloy composition adjustment can improve the corrosion resistance and mechanical properties of magnesium alloys.
OBJECTIVE: To investigate bone morphogenetic protein-2 (BMP-2) expression in the soft tissues around calcium phosphate coating magnesium alloys.
METHODS: Twenty-seven healthy adult male Japanese white rabbits were randomly divided into calcium phosphate coating group, untreated group and titanium alloy group. We implanted calcium phosphate coating and untreated AZ31B magnesium alloy screws into the rabbits’ mandible, and titanium alloy group served as the control. The animals were sacrificed after 1, 2 and 3 months, and BMP-2 mRNA expressions were measured by reverse transcription-PCR.  
RESULTS AND CONCLUSION: With time passed, the BMP-2 mRNA expression showed a gradual increase for calcium phosphate coating group and untreated group, and significant differences exited in the calcium phosphate coating group at different time points (P < 0.05). The BMP-2 mRNA expression of the untreated group was significantly higher than that of the coating group at 1 and 2 months after implantation (P < 0.05). Calcium phosphate coating can promote the mRNA expression of BMP-2, which is beneficial to the early osteogenesis and shows good biological activity.
 

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