中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3801-3805.doi: 10.3969/j.issn.1673-8225.2012.21.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

新型仿生人工神经导管诱导犬周围神经再生过程的组织学观察**★

向  杰1,2，黄继锋2，李德忠2，李世普3，闫玉华3，严琼姣3   

  1. 1南方医科大学，广东省广州市  510515；2解放军广州军区武汉总医院骨科，湖北省武汉市  430070；3武汉理工大学生物材料与工程研究中心，湖北省武汉市 
    430070
  • 收稿日期:2012-02-27 修回日期:2012-03-14 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 黄继锋，博士，教授，主任医师，硕士生导师，解放军广州军区武汉总医院骨科，湖北省武汉市 430070 huangjfgk@yahoo.com.cn
  • 作者简介:向杰★，男，1982年生，湖南省衡阳市人，汉族，南方医科大学在读硕士，主要从事周围神经损伤再生修复方面研究。 xiangjiegk@163.com
  • 基金资助:

    湖北省自然科学基金重点项目(2009CDA041)；武汉市科技攻关计划项目(200960323125)。

Histological observation of dog peripheral nerve regeneration induced by a novel biomimetic nerve conduit

Xiang Jie1,2, Huang Ji-feng2, Li De-zhong2, Li Shi-pu3, Yan Yu-hua3, Yan Qiong-jiao3   

  1. 1Southern Medical University, Guangzhou  510515, Guangdong Province, China; 2Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan  430070, Hubei Province, China; 3Biomedical Materials and Engineering Center, Wuhan University of Technology, Wuhan  430070, Hubei Province, China
  • Received:2012-02-27 Revised:2012-03-14 Online:2012-05-20 Published:2012-05-20
  • Contact: Huang Ji-feng, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan 430070, Hubei Province, China huangjfgk@ yahoo.com.cn
  • About author:Xiang Jie★, Studying for master’s degree, Southern Medical University, Guangzhou 510515, Guangdong Province, China; Department of Orthopedics, Wuhan General Hospital of Guangzhou Military Command of Chinese PLA, Wuhan 430070, Hubei Province, China xiangjiegk@163.com
  • Supported by:

     Hubei Natural Science Priority Research Fund, No. 2009CDA041*; Wuhan Science and Technology Research Program, No. 200960323125*

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摘要：

背景：前期动物实验已经证明，精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/β-磷酸三钙/神经生长因子导管可以修复大鼠坐骨神经10 mm缺损，该材料具有良好的生物相容性和细胞亲和性。
目的：观察精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管诱导犬周围神经再生过程的组织学变化。
方法：取健康成年杂种犬12只，建立30 mm腓总神经缺损模型，用精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子人工神经导管进行桥接修复。
结果与结论：修复后3个月时在神经远端即可发现新生神经纤维，随着时间的推移，再生神经轴突直径及髓鞘厚度不断增加，9个月时再生神经中可见较多发育成熟的有髓神经纤维。胫骨前肌损伤后出现逐渐萎缩，修复后6，9个月萎缩肌肉逐渐恢复。从组织学角度证实了精氨酸-甘氨酸-天冬氨酸多肽接枝聚(羟基乙酸-L-赖氨酸-乳酸)/聚乳酸/β-磷酸三钙/神经生长因子导管可修复大动物粗大神经缺损。
 

关键词: 神经导管, 神经生长因子, 聚乳酸, &beta, -磷酸三钙, 周围神经损伤, 神经再生

Abstract:

BACKGROUND: Previous research has shown that the novel arginine-glycine-aspatic acid (RGD) peptide modification of poly{(lactic acid)-co-[(glycolic acid)-alt-(L-lysine)]}/poly(d,l-lactic acid)/β-tricalcium phosphate/nerve growth factor (PRGD/PDLLA/β-TCP/NGF) composite nerve conduit can effectively repair 10-mm sciatic nerve defects of rats, while it possess good cell affinity and biocompatibility.
OBJECTIVE: To observe the histological observation of dog peripheral nerve regeneration with PRGD/PDLLA/β-TCP/NGF composite nerve conduit.
METHODS: Twelve healthy hybrid dogs were used as animal models. The conduits were employed to bridge the 30 mm defects in the peroneal nerve.
RESULTS AND CONCLUSION: Newborn regenerated myelinated nerve fibers were found in the distal section at 3 months. The diameters of myelinated nerve fibers and thickness of myelin sheath were increased day by day. Tibialis anterior muscles showed gradual atrophy after operation, which peaked 3 months after denervation. Subsequently, the atrophy of tibialis anterior muscles recovered slowly at 6 and 9 months. This study proves the feasibility of the PRGD/PDLLA/β-TCP/NGF nerve conduit for repairing large-diameter nerve defects in large animals.

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