中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2815-2819.doi: 10.3969/j.issn.1673-8225.2012.15.034

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

尿酸钠致急性痛风性关节炎模型大鼠与槲皮素的抗炎作用☆

黄敬群1，孙文娟2，王四旺1，朱妙章3，李舒烨1   

  1. 解放军第四军医大学，1药学系天然药物学教研室，3基础部生理学教研室，陕西省西安市  710032；2吉林医药学院药学系，吉林省吉林市  132013
  • 收稿日期:2011-09-07 修回日期:2011-11-20 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 王四旺，硕士，教授，博士生导师，解放军第四军医大学药学系天然药物学教研室，陕西省西安市 710032 wangsiw@fmmu.edu.cn
  • 作者简介:黄敬群☆，男，1970年生，黑龙江省哈尔滨市人，汉族，2006年解放军第四军医大学毕业，博士，主管药师，主要从事中药药理研究。 Huangjq70@163.com

Effect of quercetin on monosodium urate crystal-induced acute in?ammation in gouty arthritis rats 

Huang Jing-qun1, Sun Wen-juan2, Wang Si-wang1, Zhu Miao-zhang3, Li Shu-ye1   

  1. 1Staff Room of Natural Medicines, Department of Pharmacy, 3Department of Basic Physiology, the Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China; 2Department of Pharmacy, Jilin Medical College, Jilin  132013, Jilin Province, China
  • Received:2011-09-07 Revised:2011-11-20 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Wang Si-wang, Master, Professor, Doctoral supervisor, Staff Room of Natural Medicines, Department of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China wangsiw@fmmu.edu.cn
  • About author:Huang Jing-qun☆, Doctor, Pharmacist in charge, Staff Room of Natural Medicines, Department of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Huangjq70@163.com

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摘要：

背景：以往研究表明黄酮类化合物槲皮素具有抗氧化、抗炎和凋亡诱导等生物活性，但是对于尿酸钠结晶诱导的痛风性关节炎大鼠的治疗作用未见文献报道。
目的：验证槲皮素对尿酸钠结晶诱导的急性痛风性关节炎模型大鼠的治疗作用。
方法：将72只雄性SD大鼠随机等分为6组：分别每天灌胃100，200，400 mg/kg的槲皮素，3.0 mg/kg的吲哚美辛或等量蒸馏水(模型组和对照组)，连续7 d。第5天给药后1 h向大鼠右后肢距小腿关节腔内注射3.0 mg尿酸钠混悬液制备痛风性关节炎模型，对照组不造模。采用缚线法测定大鼠距小腿关节周径来评定炎症反应程度。第7天给药后1 h取材。
结果与结论：结果表明，槲皮素呈剂量依赖性抑制急性痛风性关节炎模型大鼠的距小腿关节肿胀程度(P < 0.01)，并且能够显著改善大鼠距小腿关节的病理改变，减轻尿酸钠结晶诱导的炎症因子白细胞介素1β、肿瘤坏死因子α、环氧化酶2、一氧化氮的水平(P < 0.05或P < 0.01)，且作用与吲哚美辛相当。说明槲皮素可通过减轻炎症反应治疗急性痛风性关节炎。
 

关键词: 槲皮素, 吲哚美辛, 痛风性关节炎, 尿酸钠, 炎症因子, 大鼠

Abstract:

BACKGROUND: Previous studies have shown that flavonoid quercetin has biological properties including antioxidative, anti-inflammatory and apoptosis-inducing activities. But its effect on monosodium urate crystal-induced in?ammation in the rats required further investigation.
OBJECTIVE: To evaluate the effect of quercetin on monosodium urate crystal-induced acute in?ammation in the rats model.
METHODS: Totally 72 male Sprague Dawley rats were randomly divided into six groups. Quercetin (100, 200, 400 mg/kg) and indomethacin (3.0 mg/kg) or equivalent distilled water was given orally once a day for 7 consecutive days throughout the experiment (model group and control group). At the 5th day, gouty arthritis model was established by intra-articular injection of 100 µL (3.0 mg) of monosodium urate crystal suspension inside the ankle joint of the right hind limb at 1 hour after administration. Control group was without modeling. The in?ammation was quanti?ed by measuring the circumference of the joint with a tie line method. At the 7th day, the rats were killed for sampling at 1 hour after administration.
RESULTS AND CONCLUSION: The present study found that quercetin had a marked inhibitory effect on edema in the experimental gouty arthritis in a dose-dependent manner (P < 0.01), and improved the evolution of the histological signs of acute inflammation in the monosodium urate crystal arthritis in rats. In addition, the quercetin appeared to suppress neutrophil recruitment, interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2 and nitric oxide by suppressing monosodium urate crystal-induced chemokine production (P < 0.05 or P < 0.01). The suppression effect was equal to indomethacin. It is clearly indicated that quercetin exerts a strong anti-in?ammatory effect and can be regarded as a useful tool for the treatment of acute gouty arthritis.

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