中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (15): 2811-2814.doi: 10.3969/j.issn.1673-8225.2012.15.033

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

海洋珍珠生物提取液对人宫颈癌Hela细胞增殖与凋亡的影响★

陈建坤1，陈  超2，刘朝奇3   

  1. 1孝感市中心医院药学部，湖北省孝感市 432100；   2三峡大学医学院药理学教研室，湖北省宜昌市  443002；3三峡大学分子生物研究所，湖北省宜昌市  443002
  • 收稿日期:2011-09-27 修回日期:2011-10-28 出版日期:2012-04-08 发布日期:2012-04-08
  • 通讯作者: 陈超，教授，硕士生导师，三峡大学医学院药理学教研室，湖北省宜昌市 443002 chaochen1954@163.com
  • 作者简介:陈建坤★，女，1984年生，湖北省云梦市人，汉族，2010年三峡大学医学院毕业，硕士，主要从事消化药理学研究。 cjk128513@yahoo.com.cn

Effects of pelagi-margarita extract on proliferation and apoptosis of human cervical carcinoma Hela cells  

Chen Jian-kun1, Chen Chao2, Liu Chao-qi3   

  1. 1Department of Pharmacology, Central Hospital of Xiaogan, Xiaogan 432100, Hubei Province, China; 2Department of Pharmacology, Medical Science College of China Three Gorges University, Yichang 443002, Hubei Province, China;     3Molecular Biological Institute, China Three Gorges University, Yichang  443002, Hubei Province, China
  • Received:2011-09-27 Revised:2011-10-28 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Chen Chao, Professor, Master’s supervisor, Department of Pharmacology, Medical Science College of China Three Gorges University, Yichang 443002, Hubei Province, China chaochen1954@163.com
  • About author:Chen Jian-kun★, Master, Department of Pharmacology, Central Hospital of Xiaogan, Xiaogan 432100, Hubei Province, China cjk128513@yahoo.com.cn

PDF

333

被引次数

4

摘要：

背景：海洋珍珠生物提取液中富含锗、硒等微量元素。
目的：观察海洋珍珠生物提取液对宫颈癌Hela细胞株增殖和凋亡的影响。
方法：MTT法检测0，6，30，45，60，75，150 mg/L海洋珍珠生物提取液对Hela细胞增殖的影响；0，6，30，60 mg/L海洋珍珠生物提取液干预Hela细胞后，流式细胞仪Annexin V和PI双染检测细胞凋亡率，PI单染法测定细胞周期，RT-PCR法测定细胞株内bcl-2，bax基因表达。
结果与结论：6~45 mg/L海洋珍珠生物提取液对Hela细胞增殖有抑制作用，表现出浓度依赖性和时间依赖性(P < 0.05)，在60~150 mg/L范围内只表现出了浓度依赖性(P < 0.05)，无时间依赖性。6~60 mg/L海洋珍珠生物提取液呈浓度依赖性促进Hela细胞凋亡，使细胞阻滞在G1期；30，60 mg/L海洋珍珠生物提取液可降低细胞内bcl-2 mRNA表达，升高bax mRNA表达。说明海洋珍珠生物提取液以浓度依赖性的方式抑制Hela细胞增殖，并通过降低bcl-2 mRNA，升高bax mRNA来诱导细胞凋亡。
关键词：海洋珍珠生物提取液；宫颈肿瘤；细胞增殖；细胞凋亡；bcl-2；bax
doi:10.3969/j.issn.1673-8225.2012.15.033

关键词: 海洋珍珠生物提取液, 宫颈肿瘤, 细胞增殖, 细胞凋亡, bcl-2, bax

Abstract:

BACKGROUND: Pelagi-margarita extract is rich in germanium, selenium and other trace elements.
OBJECTIVE: To explore the effects of pelagi-margarita extract on proliferation and apoptosis in human cervical carcinoma Hela cells.
METHODS: MTT method was used to detect the effects of different concentrations of pelagi-margarita extract on proliferation and apoptosis in cervical carcinoma Hela cells. The pelagi-margarita extract concentrations were 0, 6, 30, 45, 60, 75 and      150 mg/L. Cell apoptosis rates were detected by flow cytometry using Annexin V/Propidium Iodide double staining after treated with 0, 6, 30 and 60 mg/L pelagi-margarita extracts. Then the cell cycle was detected by flow cytometry using PI staining. Expression of intracellular bcl-2 and bax mRNA was analyzed by reverse transcription-PCR.
RESULTS AND CONCLUSION: Pelagi-margarita extract with the concentration from 6 to 45 mg/L inhibited the Hela cell proliferation in a dose-and time-dependent manner (P < 0. 05). Pelagi-margarita extract with the concentration of 60 to 150 mg/L inhibited the Hela cell proliferation in a dose-dependent manner (P < 0. 05), and the inhibition was time-independent. Pelagi-margarita extract with the concentration from 6 to 60 mg/L promoted Hela cell apoptosis in a dose-dependent manner. Cell cycle arrested in the G1 phase. Pelagi-margarita extract with the concentration of 30 and 60 mg/L decreased intracellular bcl-2 mRNA expression and increased bax mRNA expression. These findings indicate that Pelagi-margarita extract inhibits Hela cell proliferation in a dose-dependent manner, and induces Hela cell apoptosis by down-regulating bcl-2 mRNA expression and up-regulating bax mRNA expression.

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