中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (14): 2530-2533.doi: 10.3969/j.issn.1673-8225.2012.14.012

• 脂肪干细胞 adipose-derived stem cells • 上一篇    下一篇

自体脂肪源性干细胞与成纤维细胞联合尿道周围注射治疗大鼠压力性尿失禁☆

关立铭，李秀娟，陈亚萍，李  倩，吴  芸，陈  伟   

  1. 上海第五人民医院妇产科，上海市200240
  • 收稿日期:2011-12-27 修回日期:2012-03-06 出版日期:2012-04-01 发布日期:2012-04-01
  • 作者简介:关立铭☆，男，1967年生，内蒙古自治区人，博士，2001年重庆医科大学毕业，主治医师，主要从事妇产科常见病诊治工作。 guanliming1@126.com

Periurinary tract transplantation of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence

Guan Li-ming, Li Xiu-juan, Chen Ya-ping, Li Qian, Wu Yun, Chen Wei   

  1. Department of Gynaecology and Obstetrics, Fifth People's Hospital of Shanghai, Shanghai 200240, China
  • Received:2011-12-27 Revised:2012-03-06 Online:2012-04-01 Published:2012-04-01
  • About author:Guan Li-ming☆, M.D., Attending physician, Department of Gynaecology and Obstetrics, Fifth People's Hospital of Shanghai, Shanghai 200240, China guanliming1@126.com

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314

被引次数

9

摘要：

背景：干细胞增殖和分化形成成纤维细胞以及适当的结缔组织，从而实现组织的再生，治疗自体盆腔器官脱垂和压力性尿失禁是目前该领域的一个研究热点。
目的：观察自体脂肪源性干细胞与成纤维细胞治疗压力性尿失禁的可行性。
方法：建立压力性尿失禁大鼠模型，建模后1个月行自体脂肪源干细胞与成纤维细胞尿道周围注射，细胞移植后1个月测定大鼠腹压漏尿点压力，同时近端尿道组织采用苏木精-伊红染色、弹力纤维染色观察形态学改变。
结果与结论：干细胞治疗后的模型大鼠漏尿点压力升高(P < 0.01)，膀胱排空正常。尿道壁肌层增厚(P < 0.01)，弹力纤维、平滑肌含量增多(P < 0.01)。结果证实，自体脂肪源性干细胞与成纤维细胞联合尿道周围注射能明显提高压力性尿失禁大鼠腹压漏尿点压力，增强注射点尿道肌层压力，可用于压力性尿失禁的治疗。

关键词: 脂肪源性干细胞, 成纤维细胞, 压力性尿失禁, 弹力纤维, 平滑肌

Abstract:

BACKGROUND: Stem cells proliferated and differented to form fibroblasts and well-suited connective tissue, followed by tissue regeneration, which is a gradually increasing research area for treatment of pelvic organ prolapse and stress urinary incontinence.
OBJECTIVE: To investigate the feasibility of periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts for treatment of stress urinary incontinence.
METHODS: Rats were induced to develop stress urinary transplantation by postpartum vaginal balloon dilation and bilateral ovariectomy. One month later, autologous adipose-derived stem cells and fibroblasts were injected into the region surrounding the urinary tract. Another month later, urinary voiding function was assessed by conscious cystometry. At the same time, urinary tract proximal end tissue was stained by hematoxylin-eosin and elastic fibers were stained to observe morphological change.
RESULTS AND CONCLUSION: Compared with un-treated rats, rats receiving stem cell treatment exhibited increased leak point pressure (P < 0.01) with normal voiding, thickened urinary tract muscular layer (P < 0.01) and increased elastic fiber and smooth muscle content (P < 0.01). These findings suggest that periurinary tract infection of autologous adipose-derived stem cells combined with fibroblasts can be used for treatment of stress urinary incontinence because it can significantly increase abdominal leak point pressure and strengthen urinary tract muscular layer pressure.

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