中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1295-1298.doi: 10.3969/j.issn.1673-8225.2012.07.036

• 组织构建与中医药 tissue construction and traditional Chinese medicine • 上一篇    下一篇

骨蚀宁胶囊对激素性股骨头坏死模型兔凝血机制的影响☆

孙志涛1，周正新2，牛  维3，李春峰4，丁  浩5   

  1. 1广州中医药大学，广东省广州市   510405；2安徽中医学院第一附属医院，安徽省合肥市  230031；   3广州中医药大学第二附属医院，广东省广州市  510120；4太和县中医院，安徽省太和县  236607；   5芜湖市中医院，安徽省芜湖市 41000  
  • 收稿日期:2011-07-14 修回日期:2011-08-04 出版日期:2012-02-12 发布日期:2012-02-12
  • 作者简介:孙志涛☆，男，1978年生，安徽省淮南市人，汉族，广州中医药大学在读博士，主要从事中西医结合治疗髋、膝关节病的研究。 harrison0611@163.com

Effect of Gu Shi Ning capsule on the hemoglutination mechanism in steroid-induced avascular osteonecrosis of the femoral head

Sun Zhi-tao1, Zhou Zheng-xin2, Niu Wei3, Li Chun-feng4, Ding Hao5   

  1. 1Guangzhou University of Chinese Medicine, Guangzhou  510405, Guangdong Province, China; 2First Affiliated Hospital, University of Anhui Traditional Chinese Medicine, Hefei  230031, Anhui Province, China; 3Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou  510120, Guangdong Province, China; 4Taihe Hospital of Traditional Chinese Medicine, Taihe  236607, Anhui Province, China; 5Wuhu Hospital of Traditional Chinese Medicine, Wuhu  241000, Anhui Province, China
  • Received:2011-07-14 Revised:2011-08-04 Online:2012-02-12 Published:2012-02-12
  • About author:Sun Zhi-tao☆, Studying for doctorate, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China harrison0611@163.com

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摘要：

背景：骨蚀宁胶囊是丁锷教授在长期治疗激素性股骨头缺血性坏死的临床实践中，总结出的有效方剂。
目的：观察骨蚀宁胶囊对激素性股骨头坏死动物模型相关凝血指标的影响。
方法：随机选取6只白兔为正常对照组，余90只白兔利用大肠杆菌内毒素加甲强龙的方法成功制备兔激素性股骨头坏死的动物模型后随机分为骨蚀宁低剂量组[0.47 g/(kg•d)]、中剂量组[1.41 g/(kg•d)]、高剂量组[4.23 g/(kg•d)]及模型组和对照组[仙灵骨葆1.92 g/(kg•d)]，分别于用药后4，8，12周检测各项指标。
结果与结论：模型组各时段血浆血栓素B2、GMP-140和血栓调节蛋白水平与均显著高于其他组，6-keto-PGF1α显著低于其他组(P < 0.01，或P < 0.05)。随骨蚀宁治疗时间的延长，血栓素B2、GMP-140和血栓调节蛋白水平逐渐降低6-keto-PGF1α水平逐渐升高；中剂量组较低、高剂量组及对照组作用更加明显(P < 0.01，或P < 0.05)。提示1.41 g/(kg•d)剂量骨蚀宁胶囊改善兔股骨头局部组织瘀血状态，防治激素性股骨头坏死效果较好。

关键词: 骨蚀宁胶囊, 激素性, 股骨头坏死, 凝血, 模型, 兔

Abstract:

BACKGROUND: Gu Shi Ning capsule is summarized as the effective prescriptions by Professor Ding E in long-term treatment in the steroid induced avascular necrosis of the femoral head (SANFH) clinical practice.
OBJECTIVE: To observe the effect of Gu Shi Ning capsule on mechanism of blood coagulation in animal models with SANFH.
METHODS: Rabbit model of femoral head necrosis was constructed with the modified method of the escherichia coli endotoxin plus methylprednisolone. Six white rabbits were randomly selected as the normal control group, while the rest 90 rabbits were randomly divided into: high-dose group of Gu Shi Ning capsule [4.23 g/ (kg•d)], middle-dose group of Gu Shi Ning capsule   [1.41 g/( kg•d)], low-dose group of Gu Shi Ning capsule [0.47 g/( kg•d)], model group and control group. Animals in each of group at 4, 8, 12 weeks after treatment, changes of each indicator was detected.
RESULTS AND CONCLUSION: Compared with the other five groups, thromboxane B2 (TXB2), GMP-140 and thrombomodulin level were higher, but 6-keto-PGF1α was lower at each time in the model group (P < 0.01 or P < 0.05). TXB2, GMP-140 and thrombomodulin were decreased but 6-keto-PGF1α was increased with time of Gu Shi Ning treatment, which in the middle-dose group was better than the high-dose and low-dose groups (P < 0.01 or P < 0.05). It is indicated that Gu Shi Ning capsules can be effectively improve the steroid-induced femoral head necrosis in intravascular coagulation status, so as to achieve prevention and treatment SANFH role.

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