中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1285-1289.doi: 10.3969/j.issn.1673-8225.2012.07.034

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

实验用脊髓腹侧损伤撞击器的设计及应用*☆

姜树东，洪  毅，白金柱，张军卫，甄巧霞，李建军   

  1. 中国康复研究中心脊柱外科首都医科大学骨科学系，北京市   100068
  • 收稿日期:2011-11-17 修回日期:2011-12-26 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 李建军，硕士，教授，博士生导师，中国康复研究中心脊柱外科首都医科大学骨科学系，北京市 100068
  • 作者简介:姜树东☆，男，1975年生，辽宁省大连市人，汉族，2010年首都医科大学毕业，博士，主治医师，主要从事脊柱脊髓损伤外科及康复治疗的研究。whyjsd@126.com
  • 基金资助:

    国家自然科学基金青年基金(30801222)。

Design and application of a new ventral spinal cord injury impactor

Jiang Shu-dong, Hong Yi, Bai Jin-zhu, Zhang Jun-wei, Zhen Qiao-xia, Li Jian-jun   

  1. Department of Orthopaedic Surgery, China Rehabilitation Research Center for Spinal Surgery, Capital Medical University, Beijing  100068, China
  • Received:2011-11-17 Revised:2011-12-26 Online:2012-02-12 Published:2012-02-12
  • Contact: Li Jian-jun, Master, Professor, Doctoral supervisor, Department of Orthopaedic Surgery, China Rehabilitation Research Center for Spinal Surgery, Capital Medical University, Beijing 100068, China
  • About author:Jiang Shu-dong☆, Doctor, Attending physician, Department of Orthopaedic Surgery, China Rehabilitation Research Center for Spinal Surgery, Capital Medical University, Beijing 100068, China whyjsd@126.com
  • Supported by:

    the National Natural Science Foundation of China,No. 30801222*

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摘要：

背景：目前脊髓损伤的基础研究主要依赖于动物损伤模型，每种脊髓损伤模型都有其适应范围及不足之处。
目的：设计并制造实验用大鼠脊髓腹侧损伤撞击器，并在大鼠脊髓损伤实验中验证仪器的使用效果。
方法：设计脊髓腹侧损伤撞击器的图纸，并按图纸组装成成品；应用该仪器制作SD大鼠脊髓腹侧损伤模型。随机分为3组，中度损伤组用23 V电压的打击力量，重度损伤组用25 V电压打击力量，对照组在安装撞击钩后，不实施打击。
结果与结论：重度损伤组BBB评分明显低于中度损伤组(P < 0.001)。BBB评分与脊髓残留组织呈线性相关(P < 0.001)，     23 V电压力量30%的脊髓变形率可以制作中度脊髓损伤模型，25 V电压力量61%的脊髓变形率可以制作重度脊髓损伤模型。证实实验设计的脊髓腹侧损伤仪器能够制作大鼠脊髓腹侧中、重度损伤模型，损伤力量大小方便可控，脊髓形变可测。

关键词: 脊髓损伤仪器, 脊髓损伤, 大鼠, 动物模型, BBB评分

Abstract:

BACKGROUND: At present, the research on the spinal cord injury (SCI) is mainly depend on the animal model, each kind of SCI model has its adapted area and inadequate.
OBJECTIVE: To design and manufacture a ventral SCI impactor model of rats, then evaluate the performance of the device in rat SCI experiment.
METHODS: The chart paper of the ventral SCI impactor was made and then the device was established according to the chart paper. The SD rat ventral SCI model was made by the device. The models were divided into three groups randomly, moderate injury group, severe injury group and control group. Moderate injury group needed a beat of 23 V, severe injury group needed a beat of 25 V, no beat in the control group.
RESULTS AND CONCLUSION: The BBB score in the severe injury group was significantly lower than that in the moderate injury group (P < 0.001), BBB score was linearly related to spinal cord residual tissue (P < 0.001). 23 V voltage power and 30% cord deformation rate results in the moderate injury model, 25 V voltage power and 61% cord deformation rate results in the severe injury model. This ventral spinal cord injury device can reliably produce moderate and severe cord injury models, and it is used easily. The injury force is controllable and the spinal deformation is measurable.

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