中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1241-1244.doi: 10.3969/j.issn.1673-8225.2012.07.024

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

急性心肌梗死模型大鼠经血管内皮生长因子121基因治疗后的血管新生☆

寿松涛1，林美光2，毛用敏3，曹  丽4，张  丽2 ，王佩显2，崔让庄3   

  1. 天津医科大学总医院，1急诊科，2心脏科，4老年医学科超声室，天津市  300052；3天津市心血管病研究所，天津市  300051
  • 收稿日期:2011-06-11 修回日期:2011-06-22 出版日期:2012-02-12 发布日期:2012-02-12
  • 作者简介:寿松涛☆，1966年生，博士，主任医师，主要从事危重患者内分泌代谢变化方面的研究。

Angiogenesis in acute myocardial infarction rats after vascular endothelial growth factor 121 gene therapy

Shou Song-tao1, Lin Mei-guang2, Mao Yong-min3, Cao Li4, Zhang Li2, Wang Pei-xian2, Cui Rang-zhuang3   

  1. 1Department of Emergency, 2Department of Cardiology, 4Department of Ultrasonic Room of Geriatrics, Tianjin Medical University General Hospital, Tianjin  300052, China; 3Tianjin Institute of Cardiovascular Disease, Tianjin  300051, China
  • Received:2011-06-11 Revised:2011-06-22 Online:2012-02-12 Published:2012-02-12
  • About author:Shou Song-tao☆, Doctor, Chief physician, Department of Emergency, Tianjin Medical University General Hospital, Tianjin 300052, China stshou@medmail. com.cn

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摘要：

背景：血管内皮生长因子121可能是基因治疗急性心肌梗死的合适目的基因之一。
目的：观察直接心肌注射重组腺病毒人血管内皮生长因子121基因(Ad-hVEGF121)对心肌梗死大鼠心脏结构、功能及新生血管影响。
方法：将78只SD大鼠随机分为假手术组(n=18)、心肌梗死组(n=24)、Ad-hVEGF121组(n=19)和生理盐水组(n=17)，后3组大鼠经结扎左冠状动脉前降支，建立急性心肌梗死模型。Ad-hVEGF121组和生理盐水组结扎后10~15 min分别在心肌三点注射Ad-hVEGF12和生理盐水。假手术组只开胸，不结扎左冠状动脉前降支。
结果与结论：注射后2周治疗组行心脏超声检查，发现相比于假手术组，心肌梗死组、Ad-hVEGF121组和生理盐水组大鼠心肌新生毛细血管数量、体质量和左心室质量/体质量明显增加(P < 0.05或P < 0.01)，且转染rAd-VEGF基因后心肌组织毛细血管密度显著高于生理盐水组和心肌梗死组。但4组大鼠的梗死面积、心脏结构和功能接近。提示直接心肌注射Ad-VEGF121能明显促进心肌内新血管形成。

关键词: 血管内皮生长因子, 基因治疗, 急性心肌梗死, 心功能, 血管新生

Abstract:

BACKGROUND: Previous studies suggest that vascular endothelial growth factor 121 may be an optimal target gene for the treatment of acute myocardial infarction.
OBJECTIVE: To investigate effect of direct myocardial injection of adenovirus recombinant human vascular endothelial growth factor 121 gene (Ad-hVEGF121) on myocardial infracted rat heart structure, function and angiogenesis.
METHODS: Totally 78 male SD rats were randomly divided into the sham-surgery (n=18), acute myocardial infarction (n=24), Ad-VEGF121 (n=19) and normal saline (n=17) groups. Among them, left anterior descending coronary arteries of the latter three groups were ligated to prepare acute myocardial infarction models and rats were randomly selected to receive Ad-hVEGF12 or normal saline via three points in the cardiac muscle at the 10-15 minutes after ligation. The chest was exposed without ligation in the sham-surgery group.  RESULTS AND CONCLUSION: At 2 weeks after injection, cardiac ultrasound showed that, compared with the sham-surgery group, the number of new capillaries, body weight and left ventricular mass / body weight of the acute myocardial infarction, Ad-hVEGF121 and normal saline groups were obviously increased (P < 0.05 or P < 0.01), especially those received transfected rAd-hVEGF12, had higher density of blood capillaries than those of the normal saline and acute myocardial infarction groups. However, there were no obviously differences between each group in infarct size, cardiac structure or functions. The direct myocardial injection of Ad-VEGF121 can significantly promote the formation of new blood vessels within the myocardium.

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