中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1169-1172.doi: 10.3969/j.issn.1673-8225.2012.07.008

• 脊柱组织构建 spinal tissue construction • 上一篇    下一篇

神经生长因子不同给药方式对坐骨神经吻合后修复与再生的影响*★

王梨明，唐际存，辛林伟，李  强，周英琼，陈  灏，古振林   

  1. 桂林医学院附属医院四肢创伤、手外科，广西壮族自治区桂林市  540001
  • 收稿日期:2011-11-09 修回日期:2011-12-08 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 唐际存，副教授，桂林医学院附属医院四肢创伤、手外科，广西壮族自治区桂林市 541001 tangjicun@126.com
  • 作者简介:王梨明★，男，1984年生，汉族，山东省日照市人，桂林医学院在读硕士，医师，主要从事四肢创伤显微外科方面的研究。 lmwang453@163.com
  • 基金资助:

    广西壮族自治区卫生计划资助项目(z2009046)，课题名称：鼠神经生长因子鞘膜内注射对周围神经吻合后修复与再生的研究。

Effects of nerve growth factor injection via different ways on repair and regeneration of the sciatic nerve after its anastomosis

Wang Li-ming, Tang Ji-cun, Xin Lin-wei, Li Qiang, Zhou Ying-qiong, Chen Hao, Gu Zhen-lin   

  1. Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin  540001, Guangxi Zhuang Autonomous Region, China
  • Received:2011-11-09 Revised:2011-12-08 Online:2012-02-12 Published:2012-02-12
  • Contact: Tang Ji-cun, Associate professor, Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin 540001, Guangxi Zhuang Autonomous Region, China tangjicun@126.com
  • About author:Wang Li-ming★, Studying for master’s degree, Physician, Department of Limb Trauma and Hand Surgery, Affiliated Hospital of Guilin Medical University, Guilin 540001, Guangxi Zhuang Autonomous Region, China lmwang453@163.com
  • Supported by:

     Health Program of Guangxi Zhuang Autonomous Region, No. z2009046*

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摘要：

背景：神经生长因子对神经损伤后的修复有促进作用，但目前对不同用药方式的优劣性尚有争议。
目的：观察鞘膜内与局部应用神经生长因子对兔坐骨神经吻合后修复与再生的影响。
方法：将24只新西兰大白兔坐骨神经切断后再缝合，分别向兔鞘膜内或损伤局部注射30 μg神经生长因子或等量生理盐水。
结果与结论：坐骨神经损伤后12周，鞘膜内注射神经生长因子组损伤坐骨神经鞘膜增厚，神经纤维排列较整齐，与正常神经纤维差异不大；且其神经干传导速度、有髓神经纤维数目、髓鞘厚度也明显高于其他组(P < 0.05)，损伤局部注射神经生长因子组次之。说明神经生长因子具有促进外周神经吻合后修复与再生的功能，鞘膜内应用优于局部注射。

关键词: 关键词：神经生长因子, 鞘膜内, 局部注射, 给药方式, 坐骨神经损伤, 神经修复, 神经再生

Abstract:

BACKGROUND: Nerve growth factor (NGF) can promote the repair of nerve after nerve injury. However the advantages and disadvantages of different treatment methods are still unclear.
OBJECTIVE: To investigate the effects of intrathecal injection and local injection of NGF on repair and regeneration of rabbit sciatic nerve after its anastomosis.
METHODS: Sciatic nerves from 24 New Zealand white rabbits were cut-off and then sutured. NGF at 30 μg or saline at the same dose were injected intrathecally or locally into the rabbits.
RESULTS AND CONCLUSION: After 12 weeks of sciatic nerve injury, the sheath of sciatic nerve was thickened in the intrathecal injection group, nerve fibers arranged in neat rows and was similar with normal nerve fibers; the nerve conduction velocity, number of myelinated nerve fibers and myelin sheath thickness in the intrathecal injection group were obviously increased compared with the other groups (P < 0.05), the local injection group took the second place. NGF can promote the repair and regeneration of peripheral nerve after its anastomosis, and the intrathecal injection NGF is superior to local injection.

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