中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1157-1160.doi: 10.3969/j.issn.1673-8225.2012.07.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

碱性成纤维细胞生长因子可拮抗过氧化氢对软骨细胞的损伤★

赵文斌，袁雪峰，王茂朋，张国良，楚翔宇，李文凯，王  江，游洪波   

  1. 华中科技大学同济医学院附属同济医院骨科，湖北省武汉市   430030
  • 收稿日期:2011-09-07 修回日期:2011-11-02 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 游洪波，博士，副教授，华中科技大学同济医学院附属同济医院骨科，湖北省武汉市 430030 youhongbo003@yahoo.com.cn
  • 作者简介:赵文斌★，男，1985年生，山西省大同市人，汉族，华中科技大学同济医学院在读硕士，主要从事组织工程方面的研究。 tjwb525@hotmail.com

Basic fibroblast growth factors antagonize hydrogen peroxide damage to chondrocytes  

华中科技大学同济医学院附属同济医院骨科，湖北省武汉市   430030   

  1. Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430030, Hubei Province, China
  • Received:2011-09-07 Revised:2011-11-02 Online:2012-02-12 Published:2012-02-12
  • Contact: You Hong-bo, Doctor, Associate professor, Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China youhongbo003@yahoo.com.cn
  • About author:Zhao Wen-bin★, Studying for master’s degree, Department of Orthopedics, Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China tjwb525@hotmail.com

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摘要：

背景：碱性成纤维细胞生长因子是否能够拮抗氧自由基带来的软骨细胞损害尚不清楚。
目的：观察碱性成纤维细胞生长因子对过氧化氢诱导的大鼠膝关节软骨细胞损伤的拮抗作用。
方法：分别以0.05，0.1，0.2，0.4，0.8，1.6，3.2 mmol/L的过氧化氢诱导SD大鼠膝关节软骨细胞凋亡，从中选择有效的诱导凋亡浓度同时加入1 µg/L的碱性成纤维细胞生长因子进行干预。
结果与结论：MTT结果显示0.2~1.6 mmol/L的过氧化氢均可明显诱导SD大鼠膝关节软骨细胞发生凋亡，且随浓度增加，凋亡增加；Hoechst 33342染色及流式细胞仪检测发现1 µg/L的碱性成纤维细胞生长因子可拮抗过氧化氢诱导的大鼠软骨细胞凋亡。

关键词: 过氧化氢, 软骨细胞, 碱性成纤维细胞生长因子, 细胞凋亡

Abstract:

BACKGROUND: Whether basic fibroblast growth factor (bFGF) can antagonize hydrogen peroxide damage to chondrocytes is still unclear.
OBJECTIVE: To observe the antagonism effect of bFGF on the hydrogen peroxide-induced cell damage to rat knee-joint chondrocytes.
METHODS: Chondrocyte apoptosis of Sprague Dawley (SD) rat knee-joint was induced by 0.05, 0.1, 0.2, 0.4, 0.8, 1.6 and    3.2 mmol/L hydrogen peroxide. Effective concentration for induced apoptosis was chosen, and then 1 μg/L bFGF was added into it for intervention.
RESULTS AND CONCLUSION: The MTT data indicated that 0.2-1.6 mmol/L hydrogen peroxide could significantly induce apoptosis of chondrocytes of SD rat knee-joint. Apoptosis was increasing as the concentration was increased. The Hoechst33342 staining and flow cytometry results showed that 1 μg/L bFGF could antagonise the hydrogen peroxide-induced apoptosis to rat knee-joint chondrocytes.

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