中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (6): 985-988.doi: 10.3969/j.issn.1673-8225.2012.06.008

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

N-乙酰半胱氨酸拮抗6-羟基多巴胺对骨髓基质细胞的毒性作用★

张琪林，罗蔚锋，王恒会，叶  艳，朱婷鸽，刘春风   

  1. 苏州大学附属第二医院神经内科，江苏省苏州市  215004
  • 收稿日期:2011-07-23 修回日期:2011-08-05 出版日期:2012-02-05 发布日期:2012-02-05
  • 通讯作者: 罗蔚锋，男，1966年生，主任医师，硕士生导师，苏州大学附属第二医院神经内科，江苏省苏州市 215004
  • 作者简介:张琪林★，女，1984年生，江苏省苏州市人，硕士，医师。

N-acetylcysteine protects bone marrow stromal cells against the toxicity of 6-hydroxydopamine★

Zhang Qi-lin, Luo Wei-feng, Wang Heng-hui, Ye Yan, Zhu Ting-ge, Liu Chun-feng   

  1. Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China
  • Received:2011-07-23 Revised:2011-08-05 Online:2012-02-05 Published:2012-02-05
  • Contact: Luo Wei-feng, Chief physician, Master’s supervisor, Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China lwfwxx@126.com
  • About author:Zhang Qi-lin★, Master, Physician, Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China zhangqilin1984@yahoo.com.cn

PDF

286

摘要：

背景：内源性6-羟基多巴胺能参与氧化应激，N-乙酰半胱氨酸能有效抗氧化和清除自由基。
目的：探讨6-羟基多巴胺对骨髓基质细胞的毒性作用及N-乙酰半胱氨酸对其的拮抗作用。
方法：在体外培养SD大鼠骨髓基质细胞，取第3代骨髓基质细胞分别加入终浓度为0，0.05，0.1 g/L的6-羟基多巴胺和终浓度为0，0.075，0.3，1.2，4.8 g/L的N-乙酰半胱氨酸。
结果与结论：MTT检测发现0.05和0.1 g/L 6-羟基多巴胺可以明显降低骨髓基质细胞的细胞活性，而加入6-羟基多巴胺的同时加入0.3 g/L N-乙酰半胱氨酸可以显著抑制6-羟基多巴胺的毒性作用。提示抗氧化剂N-乙酰半胱氨酸可以影响6-羟基多巴胺的作用。

关键词: 毒性, 6-羟基多巴胺, N-乙酰半胱氨酸, 骨髓基质细胞, 大鼠, MTT

Abstract:

BACKGROUND: 6-hydroxydopamine, as an endogenous toxic factor in the pathogenesis of Parkinson's disease, participates in oxidative stress. N-acetylcysteine resists oxidation and removes free radicals effectively.
OBJECTIVE: To investigate the toxicity of 6-hydroxydopamine in bone marrow stromal cells and the antagonistic effect of N-acetylcysteine on it.
METHODS: Bone marrow stromal cells of Sprague-Dawley rats were cultured in vitro. Bone marrow stromal cells of passage 3 were treated with 6-hydroxydopamine with the final concentrations of 0, 0.05, 0.1 g/L and N-acetylcysteine with the final concentrations of 0, 0.075, 0.3, 1.2, 4.8 g/L, respectively.
RESULTS AND CONCLUSION: MTT assay showed that 6-hydroxydopamine (0.05 and 0.1 g/L) significantly decreased the viability of bone marrow stromal cells. This toxic effect of 6-hydroxydopamine was significantly inhibited by 0.3 g/L N-acetylcysteine. It suggests that antioxidant N-acetylcysteine may affect the toxic action of 6-hydroxydopamine.

中图分类号: