中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (4): 709-712.doi: 10.3969/j.issn.1673-8225.2012.04.032

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

骨延长患者诱导型一氧化氮合酶的表达★

胡新永1，陈建文1，王义生2   

  1. 1国家康复辅具研究中心附属康复医院矫形骨科，北京市  100176；  2郑州大学第一附属医院骨科，河南省郑州市 450052
  • 收稿日期:2011-07-02 修回日期:2011-08-30 出版日期:2012-01-22 发布日期:2014-04-04
  • 作者简介:胡新永★，男，1968年生，汉族，河南省鲁山县人，2005年郑州大学毕业，硕士，副主任医师，主要从事脊柱和四肢畸形矫正治疗。huxinyonghxy@sohu.com

Expression of inducible nitric oxide synthase in bone lengthening patients

Hu Xin-yong1, Chen Jian-wen1, Wang Yi-sheng2   

  1. 1Department of Orthopedics, Affiliated Rehabilitation Hospital of National Research Center for Rehabilitation Technical Aids, Beijing  100176, China; 2Department of Orthopedics, First Affiliated Hospital of Zhengzhou University, Zhengzhou  450052, Henan Province, China
  • Received:2011-07-02 Revised:2011-08-30 Online:2012-01-22 Published:2014-04-04
  • About author:Hu Xin-yong★, Master, Associate chief physician, Department of Orthopedics, Affiliated Rehabilitation Hospital of National Research Center for Rehabilitation Technical Aids, Beijing 100176, China huxinyonghxy@sohu.com

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摘要：

背景：骨延长的牵张刺激如何转变为骨质再生的生物学信息至今不明确。
目的：观察骨延长患者诱导型一氧化氮合酶的动态表达。
方法：单侧胫腓骨延长组患者8例，分别于手术前第3天、术后第3，7，8，11，30天、停止延长时、停止延长第3，15，30天、拆除外固定器时、拆除外固定器后30 d，检测血清诱导型一氧化氮合酶水平。并设立年龄相匹配的对照组8例。
结果与结论：骨延长组牵张操作开始第1天时(术后第8天)，血清诱导型一氧化氮合酶即开始升高，停止延长时达高峰，这一时段各个时相骨延长组血清诱导型一氧化氮合酶均显著高于对照组(P < 0.01)，停止延长3 d时的血清诱导型一氧化氮合酶亦高于对照组(P < 0.05)。提示诱导型一氧化氮合酶的高表达是骨延长的分子生物学机制之一。

关键词: 关键词：骨延长, 诱导型一氧化氮合酶, Ilizarov外固定延长器, 胫腓骨, 牵张

Abstract:

BACKGROUND: It is unknown that how to transfer the impetus of distraction into the message of bone regeneration in limb lengthening.
OBJECTIVE: To study the dynamic expression of inducible nitric oxide synthase (iNOS) in bone lengthening patients. 
METHODS: Totally eight patients whose tibia and fibula are shorten in single lower limb were involved in bone lengthening group. The serum iNOS levels of the patients were measured respectively at 3 days preoperatively, at 3, 7, 8, 11, 13 days postoperatively, stopping bone lengthening, at 3, 15, 30 days after stopping bone lengthening, removing the external lengthening apparatus and at 30 days after removing the external lengthening apparatus. Eight patients with similar age to the bone lengthening group were selected as control.
RESULTS AND CONCLUSION: The serum iNOS levels in the bone lengthening group began to increase at first day after the distraction (the eighth day after operation) and the peak values was at the stopping bone lengthening. The expression of iNOS in the bone lengthening group was significantly higher than that of the control group at every time point from the first day after the distraction to the day of stopping bone lengthening (P < 0.01). The serum iNOS levels in the bone lengthening group at 3 days after stopping the bone lengthening was also higher as compared with the control group (P < 0.05). It is indicated that the higher expression of iNOS is one of the molecular biological mechanisms of bone lengthening.

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