中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (25): 4075-4081.doi: 10.3969/j.issn.2095-4344.1783

• 干细胞综述 stem cell review • 上一篇    下一篇

骨髓间充质干细胞对机械力学微环境的响应：观点、现状、思考与未来

孙  彬，段  浩，钟宗雨，刘  政，李晓壮，唐志宏，何  飞
  

  1. 昆明医科大学第一附属医院，云南省昆明市  650032
  • 修回日期:2019-04-08 出版日期:2019-09-08 发布日期:2019-09-08
  • 通讯作者: 何飞，博士，主任医师，昆明医科大学第一附属医院，云南省昆明市 650032
  • 作者简介:孙彬，男，1992年生，山东省东平县人，汉族，昆明医科大学在读硕士，主要从骨组织工程研究。
  • 基金资助:

    国家自然科学基金项目(31460244)，项目负责人：何飞；云南省科技计划项目省应用基础研究(2015FA002)，项目负责人：何飞

Response of bone marrow mesenchymal stem cells to mechanical microenvironment: viewpoint, actuality, thinking and future

Sun Bin, Duan Hao, Zhong Zongyu, Liu Zheng, Li Xiaozhuang, Tang Zhihong, He Fei
  

  1. First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Revised:2019-04-08 Online:2019-09-08 Published:2019-09-08
  • Contact: He Fei, MD, Chief physician, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Sun Bin, Master candidate, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31460244 (to HF); the Applied Basic Research Project of Yunnan Provincial Science and Technology Plan, No. 2015FA002 (to HF)

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摘要：

文章快速阅读：

文题释义：
机械力学微环境：细胞微环境是在细胞生长发育过程中可以影响调控其增殖分化的微环境，而机械力学微环境是指细胞在生长发育过程中所能感受到周围力学刺激的微环境。
基底力学：是影响骨髓间充质干细胞增殖分化的重要因素之一，骨髓间充质干细胞会感受不同刚度的基底，根据基底刚度来抉择自身分化方向，如成神经、脂肪、肌肉、骨细胞方向都有相对应合适的刚度范围，一旦超过范围对自身的分化会产生抑制作用，很多研究者都通过构建各种不同支架模仿细胞感受基底力学环境来进一步探讨细胞与基底力学之间的关系。

 

摘要
背景：骨髓间充质干细胞是骨组织工程中重要的种子细胞，但该细胞定向增殖分化调控研究仍是一个难题，既往的研究主要集中在生物化学方面的调控作用，而机械力学因素对骨髓间充质干细胞增殖分化的调控研究较少。
目的：总结近年来机械力学微环境对骨髓间充质干细胞增殖分化影响的研究现状和最新进展。
方法：以“力学微环境、间充质干细胞、刚度、增殖分化”为关键词检索CNKI数据库、万方数据库，以“mechanical microenvironment，mesenchymal stem cell，stiffness，proliferation and differentiation”为英文关键词检索PubMed数据库，共检索到117篇文献，经筛选后对符合标准的65篇文献进行归纳分析。
结果与结论：不同的机械力可使骨髓间充质干细胞增殖分化受到不同影响。牵张力与流体剪切力对骨髓间充质干细胞产生的刺激大部分会使其向成骨方向分化，而压缩力和静水压力对骨髓间充质干细胞产生的刺激大部分会使其偏向软骨方向分化，小部分向成骨方向分化。不同基底力学机械信号对间充质干细胞成神经、成脂、成肌与成骨方向产生影响，每种分化方向都有其最适的分化条件。细胞的自身组分在响应机械力学信号中也承担重要作用。为了探索机械力学与骨髓间充质干细胞增殖分化之间的关系，模拟建立骨髓间充质干细胞所受力学微环境相似的基质和支架系统是目前重要研究手段之一。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0003-3581-2001(孙彬)

关键词: 骨髓间充质干细胞, 力学微环境, 牵张力, 流体剪切力, 刚度, 压缩力, 静水压力, 基质, 增殖分化, 国家自然科学基金

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells are important seed cells in bone tissue engineering repair. However, it is difficult to control the cell proliferation and differentiation. Although previous studies have focused on biochemical regulation, little is reported on the mechanical microenvironment for controlling the proliferation and differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To summarize the research status and recent progress in the effect of mechanical microenvironment on the proliferation and differentiation of bone marrow mesenchymal stem cells.
METHODS: A search with the keywords of “mechanical microenvironment; mesenchymal stem cells; stiffness; proliferation and differentiation” in Chinese and English was conducted in the CNKI, WanFang and PubMed, respectively. Initially 117 articles were retrieved, and 65 eligible articles were finally summarized.
RESULTS AND CONCLUSION: Different mechanical forces cause different effects on the proliferation and differentiation of bone marrow mesenchymal stem cells. Under mechanical tension and fluid shear forces, the cells mostly differentiate into osteoblasts. Under compressive and hydrostatic pressure, the majority of the cells differentiate into chondrocytes, while a small amount of the cells are induced to differentiate into osteoblasts. Mechanical signals from different basal mechanics have an effect on neurogenesis adipogenesis, myogenesis and osteogenesis in the mesenchymal stem cells. There are different optimal conditions for the cells differentiating into different tissues. Different self-components of the cells also play an important role in their response to mechanical signals. To explore the relationship between mechanical dynamics and proliferation and differentiation of mesenchymal stem cells, we establish a simulated matrix and scaffold system similar to the mechanical microenvironment of bone marrow mesenchymal stem cells.

Key words: bone marrow mesenchymal stem cells, mechanical microenvironment, tension, fluid shear stiffness, compressive force, hydrostatic pressure, matrix, proliferation and differentiation, National Natural Science Foundation of China

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